Ch. 36 - Francisella & Brucella

Question 0

Francisella tularensis disease

Answer 0

Diff forms of tularemia:

Ulceroglandular (skin lesion; arthropod)
Oculoglandar (direct contact of eye)
Pneumonic (inhalation; bioweapons)
Oropharyngeal & GI (ingestion)

Question 1

Francisella tularensis

Answer 1

Very small non-motile G-cb
Zoonotic
Capsule
Strict Aerobe
Causes tularemia (rabbit or glandular fever)
Fastidious (cysteine) 

Type *A - rabbits, cats, biting *arthropods
Type B - rodents and cats

Question 2

Francisella tularensis diagnosis

Answer 2

VERY DANGEROUS (specialized labs)
use antibody fluorescence
Ab titer 4X or more
May produce beta lactamase (use Gentamycin)

Question 3

Brucella

Answer 3

Small non-motile G-cb 
Zoonotic
No capsule
Strict aerobe
NFB

4 spp cause brucellosis (undulent fever)
B. abortus (cattle and bison)
B. melitensis (sheep and goat)
B. suis (swine) 
B. canis (canine) 

Mild/asymptomatic disease in natural host
Bacteria shed in milk, urine, birth products

Question 4

Brucella virulence

Answer 4

No exotoxin, weak endotoxin
Intracellular (macs mono) inhibit phagolysosome fusion
Granuloma formation (spleen, kidney, liver, lymph nodes, bm
Vaccination
Direct contact with org (lab), inhalation (bioweapon) ingestion

Question 5

Brucella disease

Answer 5

B. abortus, canis - mild disease, rarely supprative

B. suis - destructive lesions, prolonged infection

B. melitensis - severe disease w/ complications due to replication within phagocytes

Undulent fever
Relapses - infectious foci and inadequate therapy

Diagnosis: 
Urease + 
OXI +
Antisera ID (not canis) 
Ab detection (IgM, then IgG and IgA)