Ch 33 High-Performance Liquid Chromatography

Question 1

What are the separation mechanism of HPLC?

Answer 1

Partition/ liquid-liquid chrom
adsorption/ liquid-solid chrom
ion exchange chrom
size exclusion chrom
affinity chrom
chiral chrom

Question 2

A type of chromatography that combines a liquid mobile phase and a very finely divided stationary phase, the liquid is pressurized for efficient flow rates

Answer 2

HPLC

Question 3

Is a process in which dissolved gases are swept out of the solvent by bubbles of an inert, insoluble gas

Answer 3

Sparging

Question 4

When the solvent composition remains constant in HPLC

Answer 4

Isocratic elution

Question 5

When the solvent composition is changed continuously in HPLC

Answer 5

Gradient elution

Question 5

Found between the mobile phase container and the injector that is used to condition the mobile phase

Answer 5

Scavenger column

Question 6

Found between the column and the injector that removes particulates and other solvent impurities

Answer 6

Guard column

Question 7

A mechanism in which the stationary phase is a second liquid that is immiscible with the liquid mobile phase

Answer 7

Partition chromatography

Question 8

The stationary phase is a solvent held in place by adsorption of the surface of the packing particles

Answer 8

Liquid-liquid Partition Chrom

Question 8

The stationary phase is an organic species that is attached to the surface of the packing particles by chemical bonds

Answer 8

Liquid-bonded-phase Chrom

Question 8

The stationary phase is polar and the mobile phase is nonpolar

Answer 8

Normal-phase partition chromatography