Ch. 3 The Microscope

Question 1

Light microscope

Answer 1

Light illuminates or passes through the specimen being observed

Question 2

Compound microscope

Answer 2

• microscope used to observing small and thin objects

* light passes through 2 lenses positioned one above the other

Question 3

Binocular / dissecting microscope

Answer 3

• 2 eyepieces
• used to view larger or thicker objects

Dissecting:
• allows a 3D image
• the depth of the field of view is much larger
• the upper and lower lights (separate switches)
• maximum notification is not as large as compound light microscopes

Question 4

Ocular lenses

Answer 4

10x

Question 5

Scanning objective

Answer 5

4x

Question 6

Low power objective

Answer 6

10x

Question 7

High power objective

Answer 7

40x

Question 8

Total magnification

Answer 8

= magnification / objective

Ex: low power= 10x=100x
High power=40x=400x

Question 9

HPD

Answer 9

high power diameter

Question 10

LPD

Answer 10

low power diameter

Question 11

LPM

Answer 11

low power magnification

Question 12

HPM

Answer 12

high power magnification

Question 13

*** HPD and LPD refer to the diameter (width) of the field of view of your microscope at the high power and low power objectives respectively

What is the equation?

Answer 13

HPD= LPD X LPM / HPM

• see notes for example

Question 14

Parfocal

Answer 14

When an object is in focus with the scanning objective (4x) it will be very close to sharp focus when the high power (40x) objective is rotated into place without using the course adjustment knob to raise or lower the stage

Question 15

Depth of field

Answer 15

The space between the slide and the coverslip

Question 16

Wet mount

Answer 16

Temporary slide made by placing the material to be viewed in a drop of fluid, frequently water (or iodine) and covering it with a clear class or plastic coverslip