Ch. 16 Quiz

Question 1

What process is used to create transgenic animals?

Answer 1

Nuclear microinjection

Question 2

In the first transgenic animal experiment, which gene from rats was cloned?

Answer 2

Somatotropin

Question 3

According to the book, which protein is produced by transgenic goats?

Answer 3

Recombinant tissue plasminogen activator

Question 4

Why are embryonic stem cells important?

Answer 4

They can develop into any tissue in the body, including the germ line

Question 5

How can location effects of transgenes in animals be avoided?

Answer 5

All of the above

• by placing LCR sequences in front of the transgene
• by inclusion of insulator sequences on both ends of the transgene
• by using natural transgenes instead of the cDNA version
• by targeting the transgene to a specific location

Question 6

What is used by targeting vectors to insert transgenes at specific locations within the host gene?

Answer 6

Homologous recombination

Question 7

Which of the following systems can be used to control the expression of the transgene?

Answer 7

All of the above

• a modified Lacl repressor system
• the TetR recombinant promoter system
• use of a heat shock promoter from Drosphila
• the use of steroid receptors

Question 8

Why are Cre/loxP or Flp/FRT used in transgenic animals?

Answer 8

All of the above

• activation of a transgene by removing blocking sequences flanked by the loxP or FRT sites
• large-scale deletions and rearrangements of the chromosomes
• removal of selectable markers that are no longer needed
• creation of conditional knockout mutants

Question 9

What can be engineered to introduce transgenes into Drosphila?

Answer 9

P elements

Question 10

How can mosquito-borne diseases be controlled through biotechnology?

Answer 10

By genetically engineering mosquitoes that are resistant to the disease

Question 11

Which of the following is derived from bacteria and useful for generating transgenes or modulating gene expression?

Answer 11

phiC31 integrase

Question 12

Transgenic mosquitoes can be produced using a transposon from the cabbage looper called ___.

Answer 12

piggyBac

Question 13

Concerning transgenic mosquitoes, which of these is not a strategy for the control of disease spread?

Answer 13

All of the above are potential strategies.

• overexpression of defensin A from the mosquito’s pen immune system
• expression of phospholipase from bees
• expression of a human antibody in mosquito saliva
• production of lysozyme in the mosquito gut

Question 14

Transgenic…

Answer 14

Mosquitoes can limit the spread of diseases such as malaria and dengue

Question 15

Antisense RNA can be introduced as transgenes and used in ___.

Answer 15

Gene silencing