Ch. 15 Quiz Flashcards
Which trait can be conferred onto a plant by a transgene?
All of the above
- resistance to diseases
- enhance the nutritional value
- resistance to an insect
- protection from herbicides
What is the definition of totipotent?
Cells that retain the ability to develop into any cell type of a mature plant
What is the first and most important step in genetically engineering plants?
Identification of a gene that will confer a specific useful trait onto the plant
Which one of the following statements about the Ti plasmid is true?
All of the above
- originated from Agrobacterium tumefaciens
- has been used by genetic engineers to transfer genes into plants to confer a particular trait
- produces tumors on plant roots
- carries genes for opine uptake and metabolism, as well as genes for virulence
What advantage does an inducible promoter have over a constitutive promoter to express transgenes in plants?
Transgenes under the control of an inducible promoter are only expressed in the tissue that requires that particular trait.
What is DNA coated onto when transforming plant cells with a particle gun?
Gold
Why is either the gene for luciferase or the gene for neomycin phosphotransferase included when transforming DNA into plant cells?
These genes encode proteins that can be used to select for or identify cells that have been successfully transformed.
What is the significance of using the Cre/loxP system in plant biotechnology?
This system provides a way to remove the selectable marker or reporter gene from the transgenic plants.
Which agency is responsible for regulation of all transgenic technology?
All of the above
- FDA
- EPA
- USDA APHIS
- NIH
Which of the following statements about glyphosate is not true?
Glyphosate is toxic to the environment according to the EPA
Which of the following statements about Bt toxin is true?
All of the above are true.
- produced by the soil bacterium bacillus thuringiensis
- kills insects like cotton bollworms and corn borers
- released by the Cry proteins of bacillus spores that are ingested by insects
- transgenic crop plants expressing the Cry proteins have been very beneficial in decreasing the use of insecticides
What happened when the genes for drought and stress tolerance were placed under the control of a constitutive promoter and transformed into rice?
The growth of the rice plant was stunted
Which of the following introduce mutations in plant genes?
All of the above
- TILLING
- transposon insertions
- RNAi
- fast neutron mutagenesis
Fast neutron mutagenesis produces ___.
Deletions