Ch 12-13: Genomes and Biotechnology

Question 1

DNA’s electrical charge

Answer 1

Negative

Question 2

Restriction Enzymes

Answer 2

“Cuts/chops up” DNA into separate fragments

Question 3

Distance traveled by Longer and Shorter DNA segments in a gel

Answer 3

Shorter DNA - Encounters less resistance and therefore travels a longer distance

Longer DNA - More resistance, less distance. (Think opposites)

Question 4

A few Major uses of DNA gels

Answer 4

May be used in criminal investigations, comparing Evolutionary relationships, Medical Diagnostic tests(ex: test for huntingtons disease), forensics, DNA fingerprinting, Who’s the father!

Question 5

DNA Fingerprinting

Answer 5

Comparing DNA banding patterns between different individuals

Question 6

Restriction Fragment Length Polymorphism (RFLPs)

Answer 6

Differences in the placement of restriction sites among individuals

Question 7

Polymerase Chain Reaction (PCRs)

Answer 7

A method for creating numerous copies of one segment of DNA in a test tube.

Question 8

Short Tandem Repeats (STRs)

Answer 8

Repeating sequences of DNA; used in fingerprinting

Question 9

SNPs

Answer 9

Most common genetic difference among people

Question 10

Gel electrophoresis

Answer 10

A method of separating DNA in a gelatin-like material using an electrical field