Cellular oncogenes, growth factors, receptors, signalling and cancer Flashcards
What does MYC protein act as?
Transcription factor
Reciprocal translocation of the MYC gene and IgH promoter region is frequently observed in which cancer associated with EBV?
Burkitt’s lymphoma
The BCR/ABL translocation is associated with the philadelphia chromosome. It is frequently observed in which cancer?
Chronic myeloid leukaemia (CML)
HER2 in a complex with EGFR acts as a?
receptor tyrosine kinase
Which cancer has the highest frequency of activating K-RAS point mutations?
Pancreatic cancer
MYC protein associates with DNA in a complex that is?
Heterodimeric
External signals can promote proliferation through upregulation of which cyclin?
Cyclin D1
Herceptin is used to treat which type of cancer?
ERBB2 positive breast cancer
During standard WNT, signalling the molecule beta-catenin associated with which transcription factor?
TCF
What is Src and what does it do
Is a potent oncogene
Causes cell transformation
What extra genome does RSV carry
v-src which specifies the src protein
What is c-src and what does it do
A tyrosine kinase
Phosphorylates substrates and aids in growth factor receptor signalling
What cancer is c-src altered/mutated in
Colon cancer
What can be used to identify & confirm cellular oncogenes
Transfection
What cancer does the overexpression of ERBB2/HER2 occur in
Breast cancer (20-30%)
How many forms of MYC are there in humans
3
What cancer is N-myc often amplified in
Human childhood neuroblastoma
What cancer is BCR-ABL fusion associated with
Chronic myeloid leukaemia
What does BCR-ABL fusion lead to
Abnormal activity of ABL tyrosine kinase
What is used to treat ERBB2/HER2 positive breast cancer
Herceptin
What is the MAD network
Group of transcription factors
Interactions cause gene-specific transcriptional activation or repression
What do MYC-MAX complexes bind to
e-boxes within gene promoters
