Cell immortalisation Flashcards
What is senescence associated with
Induction of CDK inhibitors
What is crisis
A state that happens when cells lose telomeres
Resulting in end to end fusion of chromosomes
What protects telomeric DNA from degradation
Shelerin complex
How do cancer cells escape crisis
By expressing telomerase - hTERT
What makes a telomerase unique
Carries around its own RNA template
Where are telomerase expressed
Germ cells and some stem cells
What transcription factor and oncoprotein have been linked to hTERT
MYC and E6
What can repress hTERT transcription
Menin
p53
Mxd
What are HSCs associated with
Lack of expression of cell surface markers
What can sphere assays be used to identify
Cells with stem cell characteristics
What is the phenotype of immortality
Proliferate indefinitely
What is angiogenesis
The formation of new blood vessels from pre existing vasculature
What is vasculogenesis
Process in which vessels are created through the differentiation of stem cells
Senescence is associated with the induction of which protein
p16
Cell populations enter crisis because of which event
Failure to maintain telomeres
Activity of which transcription factor has been linked to an increase in hTERT
MYC
Most b-catenin is normal epithelial cells is associated with which protein
E-Chadherin
What signalling molecule is an activator of angiogenesis
VEGF-a
