Cellular Basis of Life Flashcards
What is life?
The capacity for growth and self-replication.
Molecular Dogma
DNA->RNA->Protein
Contains genetic material in a cell
Nuclear Region
Contains multiple organelles & sites of catalytic action to support metabolic activities required for growth & replication
Cytoplasm
No true nucleus
Nuclear Region
Prokaryotes
Have a real nucleus
Eukaryotes
- no true nucleus
- Fatty acids + ether linkages
- rRNA – distant from eubacteria
Archea
Cell Wall gives structure & rigidity
Prokaryotes
Contain organelles
Eukaryotes
Contains DNA packaged in proteins called chromatin
Only visible in replicating cells
Chromosomes
- Two phospholipid bi-layer’s form the Nuclear Membrane
- Studded with Nuclear Pores
- Allows transport of RNA out & signals inward
Nuclear Envelope
- “Translated” into proteins
- Exported from Nucleus
- To sites of Protein synthesis (ribosomes)
mRNA
- RNA species that links up with
AMINO ACIDS -> PROTEINS - Found in Ribosomes
tRNA
transfer RNA
- Forms a complex with proteins
- Site of protein synthesis
rRNA
ribosomal RNA
- Central in flow of genetic information
- Translate mRNA into Proteins
- Complex of rRNA, proteins, tRNA & mRNA
- Found free in cytoplasm or associated with Endoplasmic Reticulum (ER)
Ribosomes
- Intracellular fluid inside the cell (transparent)
- Mainly water, ions, biomolecules/metabolites & proteins
- Concentration Gradients of Biomolecules
- Protein Complexes
Cytosol
These make up the?
- Nuclear envelope
- Endoplasmic reticulum
- Golgi apparatus
- Lysosomes
- Vacuoles
- Plasma membrane
Endomembrane System
____ ER is studded with Ribosomes
* sites of protein synthesis & modification (glycoproteins),
* delivers protein cargo to Golgi
Rough
____ ER – no Ribosomes,
* important in Lipid synthesis & detoxification,
* traffics lipid to Golgi
Smooth
- Membrane network from nucleus into cell
- Consists of folds – Cisternae
Endoplasmic Reticulum
Directs/Sorts traffic inside the cell
Golgi Apparatus
Nuclear “receiving” side of the golgi
cis face
plasma membrane “shipping” side of the golgi
trans face
- Membrane bound compartment within the cell
- Highly acidic
- Contains degradative (hydrolytic) enzymes
Lysosome
Recycling old organelles within the cytoplasm
Autophagy
amphipathic lipid species – contains hydrophobic and hydrophilic portions
Phospho-lipid
Circular (Colloidal) arrangement of phospholipids
Micelle
____ Fatty Acid – pack together nicely, stiff, no fluidity
Saturated