Cellular Basis of Life Flashcards
What is life?
The capacity for growth and self-replication.
Molecular Dogma
DNA->RNA->Protein
Contains genetic material in a cell
Nuclear Region
Contains multiple organelles & sites of catalytic action to support metabolic activities required for growth & replication
Cytoplasm
No true nucleus
Nuclear Region
Prokaryotes
Have a real nucleus
Eukaryotes
- no true nucleus
- Fatty acids + ether linkages
- rRNA – distant from eubacteria
Archea
Cell Wall gives structure & rigidity
Prokaryotes
Contain organelles
Eukaryotes
Contains DNA packaged in proteins called chromatin
Only visible in replicating cells
Chromosomes
- Two phospholipid bi-layer’s form the Nuclear Membrane
- Studded with Nuclear Pores
- Allows transport of RNA out & signals inward
Nuclear Envelope
- “Translated” into proteins
- Exported from Nucleus
- To sites of Protein synthesis (ribosomes)
mRNA
- RNA species that links up with
AMINO ACIDS -> PROTEINS - Found in Ribosomes
tRNA
transfer RNA
- Forms a complex with proteins
- Site of protein synthesis
rRNA
ribosomal RNA
- Central in flow of genetic information
- Translate mRNA into Proteins
- Complex of rRNA, proteins, tRNA & mRNA
- Found free in cytoplasm or associated with Endoplasmic Reticulum (ER)
Ribosomes
- Intracellular fluid inside the cell (transparent)
- Mainly water, ions, biomolecules/metabolites & proteins
- Concentration Gradients of Biomolecules
- Protein Complexes
Cytosol
These make up the?
- Nuclear envelope
- Endoplasmic reticulum
- Golgi apparatus
- Lysosomes
- Vacuoles
- Plasma membrane
Endomembrane System
____ ER is studded with Ribosomes
* sites of protein synthesis & modification (glycoproteins),
* delivers protein cargo to Golgi
Rough
____ ER – no Ribosomes,
* important in Lipid synthesis & detoxification,
* traffics lipid to Golgi
Smooth
- Membrane network from nucleus into cell
- Consists of folds – Cisternae
Endoplasmic Reticulum
Directs/Sorts traffic inside the cell
Golgi Apparatus
Nuclear “receiving” side of the golgi
cis face
plasma membrane “shipping” side of the golgi
trans face
- Membrane bound compartment within the cell
- Highly acidic
- Contains degradative (hydrolytic) enzymes
Lysosome
Recycling old organelles within the cytoplasm
Autophagy
amphipathic lipid species – contains hydrophobic and hydrophilic portions
Phospho-lipid
Circular (Colloidal) arrangement of phospholipids
Micelle
____ Fatty Acid – pack together nicely, stiff, no fluidity
Saturated
____ Fatty Acids – push apart, fluidity
Unsaturated
The way in which substances enter a cell
Endocytosis
The way things are secreted out of a cell
Exocytosis
- Contain outer membrane with lots of lipids/lipoprotein & transport proteins
- Thin cell wall of peptidoglycan
- Contains periplasmic space
Gram negative
- No outer membrane
- Major Peptidoglycan (carbohydrate polymer) cell wall
- Smaller periplasmic space
Gram Positive
What kind of cells
- Have a thick cellulose cell wall
- Confers stability & protection
- Survive high osmotic pressure
Plant
- Found in all free-living eukaryotes
- Allows cellular respiration
- Has its own DNA
- Double membrane system
Mitochondria
- Plant & Algae only
- Double membrane system
- Has its own DNA
- Allows Photosynthesis
Chloroplasts
- No nucleus
- 1 “bacterial chromosome”
- Duplicates by replication
- Cell divides around this
Binary Fission
- Have nuclei & multiple chromosomes
- No Cell Walls
- Nuclear envelope dissolves
- Chromosomes duplicates & separate
- Cell divides around this
- Nuclear envelope reforms
Mitosis
Cytoplasmic events required to separate chromosomes & divide the cytoplasm
Cytokinesis
- Separation of the sister chromatids -> daughter chromosomes
- Division of the cytoplasm
- Formation of new plasma membrane -> 2 daughter cells
Cytokenisis
The protein complex’s basic unit is the
Centriole
Short Microtubules
Aster
Longer Microtubules
Spindle Microtubules
Microtubules which bind a chromosome
Kinetochore Microtubules
Breaks down connections between sister chromatids with the help of enzymes
Seperase
This is an image of the
Cytokinesis Cleavage
Division without growth
Non Mitotic phase of cell
Interphase
These are the parts of the ____ stage of the cell cycle
- G1
- S
- G2
Interphase
This are the stages of what part of the cell cycle?
- P
- (P)
- M
- A
- T
Mitosis
These are the stages of?
- Prophase
- Pro-Metaphase
- Metaphase
- Anaphase
- Telophase
Mitosis
During which phase does
- The cell grows
- The nucleus conducts DNA Replication
- The cytoplasm conducts Centrosome duplication
Interphase
During which phase does
- Sister chromatids appear in the nucleus
- Early spindle formation in the cytoplasm
Prophase
In which phase does
- The nucleus membrane dissolves
- Microtubules extend in the cytoplasm
- Microtubules attach to kinetochore
Pro-meta phase
In which phase does
- Attached chromosomes align in the nucleus
- Extension of Spindle forms symmetry & plate formation in the cytoplasm
Metaphase
In which phase does
- Separation of sister chromatids in the nucleus
- De-polymerization of Microtubules & Migration of Chromatids in the cytoplasm
Anaphase
In which phase
- Reformation of nuclear membrane (from remnants of old envelope)
- Cytokinesis & Cleavage Dissolution of Spindle in the cytoplasm
Telophase
In which phase are there
- Correct chromosome number
- 1 centrosomes
G1
In which phase are there
Double chromosome
(chromatids)
S Phase
In which phase are there
2 centrosomes
G2
Programmed Cell Death
Apoptosis
Which checkpoint happens before DNA replicaiton?
G1 Checkpoint
Which checkpoint is the Pre-mitosis check?
G2 Checkpoint
Which checkpoint checks for the correct separation of chromosomes?
M checkpoint
These are the function of
- Propagation
- Growth & embryonic development
- Repairs & Renewal
Cell Division
A special type of cell division that produces gametes – sperm & egg cells
Meiosis
- In Round 1, pairs of chromosomes separate
- In Round 2, sister chromatids separate
Meiosis
The study of the organization, structure, and function of living organisms.
Biology
The middle of the X of a chromosome
Centromere
The legs of chromosomes
Chromatids
____ is when an aerobic bacteria went into anaerobic bacteria
Endosymbiosis