Cells, Organelles, & Transport Flashcards
The basic component of the phospholipid membrane is made up of (a) __________.
A. Steroids
B. Glycerol backbone and three fatty acid chains
C. Saturated triglycerides
D. Unsaturated triglycerides
E. Phosphate head and two fatty acids
Phosphate head and two fatty acids
The phospholipid membrane is __________
Amphipathic
The term “amphipathic” regarding the phospholipid bilayer means __________.
A. It only has a hydrophobic component
B. It only has a hydrophilic component
C. It has both a hydrophobic and hydrophilic component
D. It is made up of carotenoids
E. It is made up of waxes
It has both a hydrophobic and hydrophilic component
Which component of the phospholipid bilayer is hydrophilic?
A. Phosphate head
B. Fatty acid tails
C. Glycerol backbone
D. Carotenoid
E. Wax
Phosphate head
Which component of the phospholipid bilayer is hydrophobic?
A. Wax
B. Carotenoid
C. Lanolin
D. Phosphate head
E. Fatty acid tails
Fatty acid tails
Which of the following most easily diffuses through the phospholipid bilayer?
A. Hydrophilic molecules
B. Hydrophobic molecules
C. Large polar molecules
D. Glucose
E. Ions
Hydrophobic molecules
The hierarchy of membrane permeability is as follows, from most to least permeable:
(MOST) Small, Hydrophobic Molecules: O2, CO2, N2, Steroid Hormones
Small, Uncharged, Polar Molecules: H2O, Urea, Glycerol, NH3
Large, Uncharged, Polar Molecules: Glucose, Sucrose
(LEAST) Ions: H+, Na+, HCO3–, K+, Ca2+, Cl–, Mg2+
Thus, we know that hydrophobic molecules will most easily diffuse through the phospholipid bilayer (shown below). This is because they can pass through the non-polar, inner portion of the bilayer, whereas polar and charged molecules do not easily diffuse through this inner, nonpolar portion, as they are not very soluble in it.
Which of the following cannot diffuse through the phospholipid bilayer?
A. Steriods
B. Carbon dioxide
C. Nitrogen
D. Calcium
E. Oxygen
Calcium
The hierarchy of membrane permeability is as follows, from most to least permeable:
(MOST) Small, Hydrophobic Molecules: O2, CO2, N2, Steroid Hormones
Small, Uncharged, Polar Molecules: H2O, Urea, Glycerol, NH3
Large, Uncharged, Polar Molecules: Glucose, Sucrose
(LEAST) Ions: H+, Na+, HCO3–, K+, Ca2+, Cl–, Mg2+
Thus, we know that ions (charged molecules) are least able to passively diffuse through the phospholipid bilayer (shown below). An ion’s charge makes it highly polar and thus is unable to solvate in the non-polar, inner portion of the bilayer, making ions unable to diffuse through the membrane. To transport ions across the membrane, dedicated transmembrane protein channels are needed, such as the sodium-potassium pump or proton pump.
Polar molecules pass through the phospholipid membrane if they are __________.
A. Large
B. Charged
C. Hydrophilic
D. Ions
E. Uncharged
Uncharged
The hierarchy of membrane permeability is as follows, from most to least permeable:
(MOST) Small, Hydrophobic Molecules: O2, CO2, N2, Steroid Hormones
Small, Uncharged, Polar Molecules: H2O, Urea, Glycerol, NH3
Large, Uncharged, Polar Molecules: Glucose, Sucrose
(LEAST) Ions: H+, Na+, HCO3–, K+, Ca2+, Cl–, Mg2+
While polar, if the molecule is both small enough and uncharged, then it still experiences some membrane permeability. The passive diffusion of small, uncharged, polar molecules is still possible, however, it is less energetically favorable than the passive diffusion of small, hydrophobic molecules. Therefore, given the same amount of time, less polar, uncharged molecules can cross a membrane (shown below) than small, non-polar molecules.
What is the function of cholesterol in the animal cell membrane?
A. Marker for cell recognition
B. Viral defense
C. Organizes DNA
D. Contains chromatin
E. Structural support
Structural support
Cholesterol is an amphipathic molecule—it has a single hydroxyl group, which interacts with the polar heads of surrounding phospholipids, and 4 hydrocarbon rings, which make up the non-polar portion. Cholesterol’s job is to alter membrane fluidity. Under normal to high temperatures, cholesterol stiffens the membrane, making it less fluid, providing structural support to the cell. If the membrane was too fluid and phospholipids moved too much, it would decrease the integrity of the membrane. At low temperatures, cholesterol increases membrane fluidity by preventing phospholipids from getting too close to each other, impairing cellular functions. Shown below is an image of the cell membrane and where cholesterol is located:
Which of the following is used as structural support in the membrane of prokaryotes?
A. Cholesterol
B. Hopanoids
C. Glycocalyx
D. Carotenoids
E. Sterols
Hopanoids
The glycocalyx is made up of __________.
A. Carbohydrates
B. Peptidoglycan
C. Lipopolysaccharides
D. Bacteria
E. Nucleic acids
Carbohydrates
Analyzing the word glycocalyx can help us remember what it means. The first part of the word, gylco–, should make us think “sugar,” and we know that sugar is a carbohydrate. We define the glycocalyx as a carbohydrate coat which covers the cell membrane of some animal cells and the outer face of the cell wall of some bacteria. It consists of both glycolipids and glycoproteins. In the TEM image below, the “furry” fringe around the cell are the carbohydrates protruding from their lipid or protein anchors.
The glycocalyx is present in some __________.
A. Animal cell plasma membrane
B. Bacterial cell wall
C. Gram-positive bacterial cell wall
D. Plant cell wall
E. Animal plasma membrane and bacterial cell wall
Animal plasma membrane and bacterial cell wall
The glycocalyx can do all the following except __________.
A. Adhere to the cell
B. Protect the cell from infection
C. Cover the plasma membrane of some animal cells
D. Act as a marker for cell-cell recognition
E. Structural support
Structural support
The glycocalyx (shown below) directly adheres to the cell because both glycolipids and glycoproteins are integrated into the membrane. It is found in both some animal and some bacterial cells. Different kinds of cells have unique sugar signatures from the glycocalyx, allowing for cell-cell recognition. The glycocalyx can also help mask adhesion points for potential cell invaders, thus preventing infection. On the other hand, especially in bacteria, the glycocalyx helps form a slime layer, which is mucus-like and sticky, promoting adhesion. Because the glycocalyx anchors to the exterior of the cell, it does not provide structural support.
The glycocalyx is made up of __________.
A. Glycolipids and glycoproteins
B. Glycolipids and cholesterol
C. Glycolipids and ribosomes
D. Glycolipids and glycolipids
E. Glycolipids and DNA
Glycolipids and glycoproteins
The glycocalyx is the multifunctional extracellular carbohydrate coat found in both some animal and some bacterial cells. These carbohydrates anchor to the outer leaflet of the cell membrane or cell wall via either a protein or lipid, thus making the glycocalyx consist of glycolipids and glycoproteins, as shown below:
Which of the following are common cell surface markers used regularly for cell-cell recognition?
A. Glycoproteins
B. Glycolipids
C. Glucocorticoids
D. Phospholipids
E. Steroids
Glycoproteins
The animal cell membrane is made up of __________.
A. Phospholipid membrane, carotenoids, glycocalyx
B. Phospholipid membrane, waxes, glycocalyx
C. Phospholipid membrane, cholesterol, glycocalyx
D. Phospholipid membrane, sterol, glycocalyx
E. Phospholipid membrane, hopanoid, glycocalyx
Phospholipid membrane, cholesterol, glycocalyx
Which of the following is used as structural support in the membrane of plants?
A. Sterols
B. Cholesterol
C. Peptidoglycan
D. Glycocalyx
E. Carotenoids
A. Sterols
If an organism has sterols to add structural support to its cellular membrane, then it is most likely a __________.
A. Plant
B. Mammal
C. Prokaryote
D. Bacteria
E. Virus
Plant
If an organism has hopanoids to add structural support to its cellular membrane, then it is most likely a __________.
A. Mammal
B. Prokaryote
C. Virus
D. Planet
E. Bird
Prokaryote
Which of the following possess centrioles?
A. Plant cells
B. Animal Cells
C. Bacteria
D. Fungi
E. Archaea
Animal Cells
Microtubules are made up of __________.
A. Keratin
B. Tubulin
C. Actin
D. Collagen
E. Clathrin
Tubulin
Microfilaments are made up of __________.
A. Keratin
B. Tubulin
C. Actin
D. Collagen
E. Intermediate filaments
C. Actin
A transmembrane protein is __________.
A. Not attached to the cellular membrane
B. Loosely attachment to the cellular membrane
C. Embedded in the nucleus
D. Embedded in the cellular membrane
E. Embedded from one side to the other side of the cellular membrane
Embedded from one side to the other side of the cellular membrane
A transmembrane protein is a type of __________.
A. Peripheral protein
B. Integral protein
C. Glycolipid
D. Glycoprotein
E. Cholesterol
Integral protein
Which of the following does the cell use to distinguish between self and foreign pathogens?
A. Channel proteins
B. Recognition proteins
C. Porins
D. Carrier proteins
E. Transport proteins
Recognition protein
Glycoproteins in animal cells have an attached __________.
A. Lipid
B. DNA
C. Oligosaccharide
D. Ion
E. Water molecule
Oligosaccharide
The major-histocompatibility complex present on macrophages can be detected by __________.
A. Channel proteins
B. Porins
C. Carrier proteins
D. Transport proteins
E. Recognition proteins
Recognition proteins
Damage to recognition proteins on animal cells will cause which of the following?
A. Inability for water to pass through the plasma membrane
B. Inability for small ions to pass through the plasma membrane
C. Inability to distinguish self and foreign cells
D. Inability to adhere to other neighboring cells
E. Inability to bind to hormones
C. Inability to distinguish self and foreign cells
Due to this membrane protein, the rate of water movement in the kidney can be increased.
A. Channel protein
B. Recognition protein
C. Carrier protein
D. Transport protein
E. Porin
Porin
Which of the following is the least specific membrane protein?
A. Recognition protein
B. Carrier protein
C. Transport protein
D. Porin
E. Receptor protein
Porin
Porins have two classes—general and specific. General porins (shown below) allow for the passage of other small, polar molecules which solvate in water as they pass through the pore. Specific porins use a selectivity filter to only allow the passage of one specific small, polar molecule, which is facilitated by certain amino acid residues which form the selectivity filter in the pore. Recognition proteins are specific, allowing for the identification of self vs non-self. Carrier proteins transport a specific molecule across the membrane. Transport proteins are the broad class of proteins containing both channel and carrier proteins, and thus can be specific to a certain molecule. Finally, receptor proteins are very specific for certain ligands.
Which of the following changes physical shape after binding to a specific molecule?
A. Channel protein
B. Carrier protein
C. Porin
D. Recognition protein
E. Adhesion protein
Carrier protein
Carrier proteins (shown below) impart selectivity on what they are transporting through undergoing a conformational change. The extracellular binding domain only binds a specific ligand. When that ligand binds to the correct binding domain, it causes a conformational change in the intracellular portion of the carrier protein, allowing the ligand to flow through the protein, into the cell, closing the extracellular domain behind it, so that no other molecules can diffuse through the membrane.
Which of the following can use ATP to move molecules?
A. Porin
B. Channel protein
C. Transporter protein
D. Facilitated diffusion
E. Passive diffusion
Transporter protein
The term transporter protein is synonymous with carrier protein, as shown below. This class of proteins undergoes a conformational change to port molecules across the membrane. There are two classes of transporter/carrier proteins—the first-class undergoes active transport and moves molecules against their concentration gradient by energy, such as from ATP and the second undergoes passive transport without an input of energy, moving molecules down their concentration gradient.
Which of the following attaches cells to neighboring cells and provides stability with internal filaments and tubules?
A. Porin
B. Channel protein
C. Adhesion protein
D. Receptor protein
E. Recognition protein
Adhesion protein
There are four general classes of cell-cell junctions in animal tissues: Anchoring Junctions, Occluding Junctions, Channel-Forming Junctions, and Signal-Relaying Junctions. Anchoring Junctions are those that anchor adjacent cells to one another using adhesion proteins, which include cadherin on the cell surface, which then interface with anchoring proteins B-catenin, p120-catenin, and others on the cadherin’s intracellular domain to link the cadherin to internal actin and intermediate filaments, as shown below:
Which of the following serves to bind to hormones and other chemical trigger molecules?
A. Porin
B. Receptor protein
C. Recognition protein
D. Channel protein
E. Adhesion protein
Receptor protein
Receptor proteins are a class of cell surface molecules that receive extracellular ligand signals, from molecules such as hormones, cytokines, short peptides, etc. and propagate the signal intracellularly via a signaling cascade. Examples of these receptor proteins include RTKs (receptor tyrosine kinases) and GPCRs (G-protein coupled receptors). Shown below is the Heterotrimeric G-protein activation/deactivation cycle in the context of GPCR signaling:
Which of the following is found in the kidneys and plant root cells?
A. Porin
B. Sterol
C. Cholesterol
D. Hopanoids
E. Glycocalyx
Porin
Due to a genetic disorder, a patient is lacking amelogenin, which allows tooth enamel to stabilize and develop its structure. Which of the following membrane proteins is affected by the genetic disorder?
A. Porin
B. Channel protein
C. Adhesion protein
D. Receptor protein
E. Carrier protein
Adhesion protein
Transporter proteins use which of the following to transport material?
A. Active transport
B. Passive diffusion
C. Facilitated diffusion
D. Active transport and facilitated diffusion
E. Facilitated and passive diffusion
Active transport and facilitated diffusion
Which of the following can use ATP?
A. Facilitated diffusion
B. Simple diffusion
C. Osmosis
D. Transporter/Carrier proteins
E. Porins
Transporter/Carrier proteins
Channel proteins create a passage for which of the following substances?
A. Nonpolar
B. Polar
C. Hydrophobic
D. Small molecules
E. Non-charged molecules
Polar
A peripheral protein is attached by __________.
A. Loose attachment to the cellular membrane
B. Embedded in the cellular membrane
C. Embedded from one side to the other side to the cellular membrane
D. Not attached to the cellular membrane
E. Cooperativity of cellular membrane
An integral protein is __________.
A. Embedded in the cellular membrane
B. Embedded from one side to the other side of the cellular membrane
C. Loosely attached to the cellular membrane
D. Not attached to the cellular membrane
E. Cooperativity of the cellular membrane
Embedded in the cellular membrane
Which of the following is used to store and protect the DNA in the eukaryotic cell?
A. Nucleus
B. Nucleoid
C. Histone
D. Nucleolus
E. Ribosome
Nucleus
Which of the following aids in organizing DNA into chromatin?
A. Histones
B. Glycocalyx
C. RNA
D. Ribosomes
E. Nucleolus
Histones
When DNA is wrapped into a bundle with eight histone proteins, it is a __________.
A. Nucleosome
B. Chromatid
C. Chromosome
D. Nucleus
E. Nucleoid
Nucleosome
Which of the following is used for ribosome synthesis in the animal cell?
A. Nucleoid
B. Nuclear envelope
C. Nuclear pores
D. Nucleolus
E. Nucleoplasm
Nucleolus
Which of the following is used to synthesize ribosomes in the eukaryotic cell?
A. mRNA
B. rRNA
C. tRNA
D. DNA
E. Protein
rRNA
A toxin inhibits the nucleolus from functioning in the eukaryotic cell. Which processes will be directly affected by this?
A. RNA synthesis
B. DNA synthesis
C. DNA organization
D. Ribosome synthesis
E. mRNA synthesis
Ribosome synthesis
Which of the following best describes the difference between mRNA and rRNA?
A. mRNA is used in the nucleolus
B. mRNA is used to carry specific amino acids
C. rRNA is not used in the nucleolus
D. rRNA is the code used to create a protein
E. rRNA is used to create ribosomes
rRNA is used to create ribosomes
Which of the following covers the outside of the eukaryotic nucleus?
A. Nuclear lamina
B. Nucleolus
C. Chromatin
D. Nuclear envelope
E. Nucleoid
Nuclear envelope
The eukaryotic nuclear envelope contains __________.
A. Nuclear pores
B. Nuclear lamina
C. Nucleolus
D. Nucleoid
E. Chromatin
A. Nuclear pores
Remember: the terms nuclear membrane and nuclear envelope are synonymous. This question can be tricky because it is asking what is contained within the nuclear envelope, rather than just closely associated structures. The nuclear lamina consists of intermediate filaments (IFs) called lamins, which closely associate to the nuclear membrane’s inner leaflet via the anchoring protein emerin. IFs are highly flexible and provide the nucleus with structural support. The nucleolus is a region within the nucleus responsible for rRNA production/ribosomal biogenesis. The nucleoid region is present in prokaryotes which do not have a proper nucleus. Chromatin is the complex of DNA and proteins (histones, which organize DNA) and is the uncondensed (not a chromosome) form in the nucleus during interphase. Nuclear pores are found in the nuclear envelope, as shown below, and allow for the transport of proteins into and out of the nucleus—proteins often require transporter chaperones to pass through the pores.
To transport mRNA and some proteins out of the nucleus, the nucleus has __________.
A. Nuclear lamina
B. Nuclear pores
C. Nucleoid
D. rRNA
E. Nucleolus
Nuclear pores
Nuclear pores are found in the nuclear envelope (shown below) and allow for the transport of proteins into and out of the nucleus—proteins often require transporter chaperones to pass through the pores. These proteins are called importins and exportins, respectively.
When there are multiple nucleosomes coiled together, they are a __________.
A. Chromosome
B. Chromatin
C. Histone
D. Nucleus
E. Nucleoid
Chromatin
Which of the following functions structurally support the eukaryotic nucleus and regulate DNA replication?
A. Nuclear lamina
B. Nuclear envelope
C. Nucleoid
D. Histones
E. Chromosomes
Nuclear lamina
Which of the following makes up the nuclear lamina of eukaryotic cells?
A. RNA and protein
B. Intermediate filaments and proteins
C. Microtubules and proteins
D. Actin filaments and proteins
E. Microfilaments and proteins
Intermediate filaments and proteins
Eukaryotic cells have a nucleus and prokaryotic cells have a __________.
A. Nuclear lamina
B. Nucleoid
C. Nucleosome
D. Chromatin
E. Chromosome
Nucleoid
Which of the following aids in chromatin organization?
A. Gills
B. Chromosomes
C. Histones
D. Nuclear envelope
E. Nucleolus
Histones
Similar to the cellular cytoplasm, the nucleus has (a) __________.
A. Nucleoid
B. Nucleolus
C. Nucleoplasm
D. Nuclear envelope
E. Histones
Nucleoplasm
All of the following can possess a cell wall EXCEPT one. Which one is the EXCEPTION?
A. Bacteria
B. Plant cells
C. Fungal cells
D. Archaea
E. Animal cells
Animal cells
A majority of the genetic information of prokaryotes are contained by the __________.
A. Nucleus
B. Nucleoid
C. Nuclear lamina
D. Nucleolus
E. Nucleoplasm
Nucleoid
All the following are cell structures EXCEPT one. Which one is the EXCEPTION?
A. Mitochondria
B. Chloroplast
C. Cell membrane
D. Nucleus
E. Cytoplasm
Cytoplasm
The metabolic and transportation activity occurs in which area of the cell?
A. Mitochondria
B. Golgi apparatus
C. Nucleus
D. Cytosol
E. Cytoplasm
Cytoplasm
What is the difference between the cytoplasm and the cytosol?
A. The cytoplasm is the gel-like substance in the eukaryotic cell
B. The cytoplasm includes the organelles
C. The cytosol includes the organelles
D. The cytosol includes only the nucleus
E. The cytosol includes only the free-floating ribosomes
The cytoplasm includes the organelles
Ribosomes are physically made up of __________.
A. mRNA and protein
B. DNA and protein
C. tRNA and protein
D. rRNA and protein
E. Chromatin and protein
rRNA and protein
For eukaryotic cells, the subunits that make up ribosomes are __________.
A. mRNA + tRNA
B. rRNA + tRNA
C. 60S + 40S
D. 50S + 30S
E. 70S
60S + 40S
For prokaryotic cells, the subunits that make up ribosomes are __________.
A. mRNA + rRNA
B. tRNA + rRNA
C. 80S
D. 60S + 40S
E. 50S + 30S
50S + 30S
If a cell has a larger Svedberg unit value, then it generally is __________.
A. Lighter
B. Larger
C. Bacteria
D. A protist
E. A prokaryote
Larger
The movement within the cytoplasm is called __________.
A. Cytoplasmic streaming
B. Power stroke
C. Systolic number
D. Diastolic number
E. Svedberg unit
Cytoplasmic streaming
The organelle that assembles glycoproteins is called the __________.
A. Rough endoplasmic reticulum
B. Smooth endoplasmic reticulum
C. Mitochondria
D. Lysosome
E. Golgi apparatus
Rough endoplasmic reticulum
If an endoplasmic reticulum has ribosomes embedded on its surface, then it most likely __________.
A. Produces mRNA
B. Produces DNA
C. Produces glycoproteins
D. Produces lipids
E. Produces hydrogen peroxide
Produces glycoproteins
The organelle that produces lipids and steroid hormones is called the __________.
A. Lysosome
B. Peroxisome
C. Golgi apparatus
D. Rough endoplasmic reticulum
E. Smooth endoplasmic reticulum
Smooth endoplasmic reticulum
If liver cells are severely damaged and unable to function, then the smooth endoplasmic reticulum is unable to:
A. Produce glycoproteins
B. Break down toxins
C. Transport substances
D. Produce hydrogen peroxide
E. Provide cell structure support
B. Break down toxins
The sarcoplasmic reticulum is located in __________.
A. Cardiac muscle
B. Smooth muscle
C. Cardiac and smooth muscle
D. Striated muscle
E. Striated and smooth muscle
Striated and smooth muscle
Which of the following is released by the sarcoplasmic reticulum?
A. Chloride ions
B. Calcium ions
C. Phosphorus ions
D. Hydrogen ions
E. Bicarbonate ions
Calcium ions
The sarcoplasmic reticulum is a specialized form of the endoplasmic reticulum found in striated and smooth muscle. It serves as a reservoir for Ca2+ cations which are released to help expose myosin-binding sites on actin filaments during muscle contraction. Troponin is the molecule to which Ca2+ binds, which then causes the tropomyosin fiber to be moved out of the major groove of actin, exposing myosin binding sites. Shown below is an image of excitation-contraction coupling in a skeletal muscle contraction:
Which of the following organelles produces lysosomes?
A. Peroxisome
B. Golgi apparatus
C. Smooth endoplasmic reticulum
D. Rough endoplasmic reticulum
E. Nucleus
Golgi apparatus
The lysosome forms when vesicles filled with proteins bud off from the Trans Golgi Network of the Golgi apparatus (shown below) and fuse together. These vesicles are filled with enzymes to degrade proteins, lipids, carbohydrates, and nucleic acids. Also important are the H+ pump proteins which are sent from the Golgi to acidify the lysosome. This slightly acidic environment inside of the lysosome is important to ensuring the pH-sensitive enzymes do not become active before reaching the slightly acidic environment of the lysosome, as well as serving as a signaling device for receptors to release their cargo once they fuse with the lysosome.
This organelle has digestive enzymes that break down molecules.
A. Golgi apparatus
B. Lysosome
C. Peroxisome
D. Smooth endoplasmic reticulum
E. Rough endoplasmic reticulum
Lysosome
The lysosome forms when vesicles filled with proteins bud off from the Trans Golgi Network of the Golgi apparatus and fuse together. These vesicles are filled with enzymes to degrade proteins, lipids, carbohydrates, and nucleic acids. Also important are the H+ pump proteins which are sent from the Golgi to acidify the lysosome. This slightly acidic environment inside of the lysosome is important to ensuring the pH-sensitive enzymes do not become active before reaching the slightly acidic environment of the lysosome, as well as serving as a signaling device for receptors to release their cargo once they fuse with the lysosome. The image below shows lysosomes digesting materials taken into the cell and recycling intracellular materials:
Which of the following organelles functions by packaging and transporting substances in vesicles?
A. Peroxisome
B. Golgi apparatus
C. Smooth endoplasmic reticulum
D. Rough endoplasmic reticulum
E. Vacuole
Golgi apparatus