Cells & Microscopy

Question 1

Wavelength Of visible spectrum

Answer 1

400-700nm

Question 2

For wax embedding, sections must be…

Answer 2

Fixed & dehydrated

Question 3

Fixing tissue means…

Answer 3

preserving it in a particular state

Question 4

Ideally, choose a fixative that…

Answer 4

Prserves features we want to observe

Question 5

Staining is used to…

Answer 5

reveal things not obvious in live cell

Question 6

A flourescent substance absorbs light of one wavelength, and emits

Answer 6

light of longer wavelength

Question 7

Flourescence microscopy can

Answer 7

make use of naturally flourescent pigments such as chlorophyll

Question 8

Confocal microscopy is a type of flourescent microscopy that produces an image from

Answer 8

a defined plane in the specimen (produces very sharp images)

Question 9

Resolution (resolving power) definition:

Answer 9

The ability to see two structures that are very close together as seperate structures

Question 10

Resolving power is measured as a …… with …… distances representing a better resolution

Answer 10

length

smaller

Question 11

Best resolution of light microscope

Answer 11

200nm

Question 12

theoretical limit resolution of transmission electron microscopy

Answer 12

0.002nm

Question 13

Sizes Of:
ribosome
e.coli cell
mitochondrion
nucleus diameter
typical eukaryotic cell

Answer 13

30nm
1um
1-2um long, 0.5 um wide
10um