Cells and organelles Flashcards
Structure of plasma membrane
-phospholipid bilayer
Function of plasma membrane
-defines the boundary of cell
-regulates entry and exit of chemicals
Structure of cytosol
-aqueous environment within the plasma membrane
Function of cytosol
N/A
Structure of cytoplasm
-cytosol plus organelles
Function of cytoplasm
N/A
Structure of cytoskeleton
consists of three types of protein polymers:
-actin filaments
-microtubules (tubulin)
-intermediate fibres
Function of cytoskeleton
-mechanical strength of cell
-control of shape
-facilitation of movement
-guide movement within cells
-interaction with other cells
Structure of nucleus
-surrounded by nuclear membrane
-largest organelle
-visible by light microscopy size: 3-10μm
Function of nucleus
-storage of chromosomes
-site of DNA replication
-site of gene expression
Structure of nucleolus
-region of nucleus
Function of nucleolus
-site of rRNA synthesis and ribosome biogenesis
Structure of nuclear membrane/envelope
-double phospholipid bilayer (inner and outer nuclear membrane
-perinuclear space between bilayers
-nuclear lamina is protein layer underneath the inner nuclear membrane
Function of nuclear membrane/envelope
-regulates the entrance and exit of mRNA and proteins
-maintains the integrity of the nucleus
Structure of mitochondria
-surrounded by membrane
-also second inner membrane that is folded into cristae that increases its surface area
-only visible by EM; size 0.5-2μm long
-number per cell reflects metabolic activity
-contains DNA that encodes ~1% of the mitochondrial specific proteins
Function of mitochondria
-aerobic oxidation of glucose to generate ATP (Krebs cycle)
-components of Electron Transport Chain are located in the inner membrane
-involved in oxidation of fatty acids
-involved in gluconeogenesis
-involved in regulated intrinsic apoptotic pathway
Structure of RER
-membrane bilayer surrounded endosomes
-membrane and inner space (lumen) continuous from the outer nuclear membrane
Function of RER
-coated with ribosomes
-mRNA translation and synthesis of proteins for secretion or insertion into cell membrane
-proteins are folded; Cys-Cys bridges form
-vesicles are budded from RER and transported to the Golgi body
Structure of SER
-membrane bilayer surrounded endosomes
-continuation of RER
Function of SER
-biosynthesis of membrane lipids and steroids
-start of N-linked glycosylation
-detoxification of xenobiotics (e.g. P450 system)
Structure of ribosome
Protein – rRNA complex
Function of ribosome
-assembly responsible for the translation of mRNA and the synthesis of proteins
Structure of Golgi body
-consists of 4-8 closely stacked, membrane-bound channels (cisterna)
-protein containing vesicles delivered from the RER
Function of Golgi body
-directs new proteins within vesicles to their correct location in cell
-transport of membrane lipids around cell
-creates lysosomes
-site of glycosylation modifications (modifying N-linked carbohydrates, glycosylation of O-linked carbohydrates and lipids)
-synthesis/packaging materials to be secreted