Cells Flashcards
Alpha 1
Agonist - phenylephrine
Vasoconstriction
Relax GI smooth muscle
Salivary secretions
Hepatic glycogenolysis
Alpha 2
Agonist - clonidine
Mainly presynaptic to inhibit transmitter release
Inhibits insulin
Platelet aggregation
Beta1 receptor
Agonist dobutamine
Mainly in heart
Increase heart rate and force
Beta 2 receptor
Agonist salbutamol
Vasodilator
Bronchodilation
Relaxation GI muscle
Beta 3 receptor
Lipolysis
Adrenoreceptors
G coupled
Alpha 1 activates phospholipase 3- IP3 - DAG
Alpha 2 inhibits adenylate cyclase
Beta stimulate adenylate Cyclase
Southern blotting
DetectsDNA
Northern blotting
Detects RNA
Western blotting
Detects and quantifies proteins
PCR
Only need one strand
Reverse transcriptase PCR RNA->DNA
Analyse mRNA