Cell Injury And Death Flashcards
What components make up the necrosome?
RIPK1, RIPK3, MLKL
What is the key regulatory step for necroptosis?
Low levels of Cas 8
What is the main mediator for necropstosis?
MLKL
What are the effector caspases?
3, 6, 7
What are the key initiating caspases for extrinsic and intrinsic apoptosis?
Extrinsic: 8
Intrinsic: 9
Which caspase is a marker of apoptosis?
3
Anti-apoptotic BCL2 proteins
BCL2, BCLW, BCLXL, A1, MCL1
Pro-apoptotic sensitizer BCL2 proteins
BID, BIM, BAD, BIK, BMF, HRK, NOXA, PUMA
Pro-apoptotic effector BCL2 proteins
BAK, BAX, BOK
Initiation of extrinsic apoptosis
Ligand/death receptor
Initiation of intrinsic apoptosis
Mitochondria secondary to stress, DNA damage
Which BCL proteins form the MOMP?
BAK, BAX, BOK
Components of the apoptosome
APAF1 and cytochrome C
What are SMAC/Diablo?
Inhibit inhibitors of apoptosis proteins (less inhibition of caspases), intrinsic pathway
Activator of caspase 10
Perforin/granzyme
Formation of the inflammasome is critical for which pathway?
Pyroptosis
Endpoint of pyroptosis
Inflammation
Endpoint of ferroptosis
Lipid peroxidation
Endpoint of parthanatos
Large DNA cleavage
Key component of parthanatos
PARP1, AIF, macrophage MIF
Lysosome-dependent cell death endpoint
Severe: necrosis
Mild: apoptosis
Lysosome-dependent cell death key component
Cathespins
Immunogenicity cell death key components
DAMPs (calreticulin, ATP, HMB1, cancer cell-derived nucleic acids, annexin 1, extracellular ATP)
Immunogenicity cell death endpoint
Apoptosis and inflammation
Entosis mechanistic components
Cadherin, loss of attachment, actomyosin-dependent
Autophagy formation process
ULK1 complex interacts with VSP34 (PI3K) complex
Autophagy elongation proteins
LC3 (Atg8) and PE
Marker of autophagy
LC3
Autophagy stimulation
Starvation (amino acid insufficiency) —> lack of mTOR
