Cell Fractionation Flashcards
What are the stages of cell fractionation?
Homogenisation. Filtration, Ultracentrifugation
What is the blended solution called?
Homogenate
What are the 3 things the solution needs to be?
Ice-cold, have a pH buffer, isotonic
Why does the solution need to be ice-cold?
To prevent enzyme activity
Why does the solution need a pH buffer?
To prevent proteins and enzymes denaturing
Why does the solution need to be isotonic?
To prevent osmosis and stop cells bursting or shrivelling
What does filtration consist of?
Homogenate is filtered to remove large debris and unbroken cells
What does filtration create?
A filtered solution
What piece of equipment is the main part of ultracentrifugation?
Centrifuge
How is the centrifuge used?
Spun at increasingly fast speeds to remove certain elements of the cell
What is the order of separation in the cell?
Nuclei, Mitochondria, Chloroplast, Lysosome, Ribosomes
