cell division Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

what is the phase of the cell growth cycle in which the cell spends most of its time in?

A

interphase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Once the cell has grown enough, it begins to replicate its DNA in the _ phase

A

S

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

how does the cell prepare itself for division through?

A

mitosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

how does the cell split itself into daughter cells through?

A

cytokinesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what is the cycle of growth and division called?

A

cell cycle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what are the 4 phases of the cell cycle?

A

G1
S
G2
M

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what phases encompass interphase?

A

G1
S
G2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what phase occurs right after mitosis?

A

G1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what is the purpose of the G1 phase?

A

acts as a gap for the cell before needing to replicate its DNA and prepare again for cell division

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what is the S phase know for?

A

synthesis of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what happens during the S phase?

A

cell doubles its DNA in preparation for division

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what is the gap between the S and M phase?

A

G2 phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what is the M phase?

A

mitosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what is the G0 phase?

A

cell becomes senescent, cell doesn’t grow or synthesize more DNA and remains “asleep” until it rejoins the cycle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what is the restriction point?

A

cell cycle checkpoint that occurs in G1 of the cell cycle in animal cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

The “commitment” step of this restriction point involves the formation of ___________________ that allow the cell to move on to the next phase.

A

cyclin/CDK complexes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

what cyclin/CDK complexes form in the G1 phase?

A

S-phase cyclin complexes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

what cyclin/CDK complexes form in the G2 phase?

A

M-phase cyclin/CDK complexes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

which checkpoint prevents the cell from going into mitosis before DNA synthesis has been completed?

A

unreplicated DNA checkpoint

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

what checkpoint makes sure that the newly replicated DNA has maintained its integrity?

A

G2 DNA-damage checkpoint

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

in which phase is there a spindle assembly and chromosome segregation checkpoint?

A

M phase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

what is the purpose of cyclin/CDK complexes?

A

proteins come together and form a functional enzyme that can then modify downstream target proteins, helping the cell progress through the cell cycle.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

list the cyclins and CDKs associated with the G1 phase

A

cyclin D
CDK 4
CDK 6

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

list the cyclins and CDKs associated with the S phase

A

cyclin E
cyclin A
CDK 2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

list the cyclins and CDKs associated with the G2 phase

A

cyclin A
CDK 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

list the cyclins and CDKs associated with the M phase

A

cyclin B
CDK 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

what is the purpose of tumor suppressors?

A

proteins that prevent the unregulated growth/division of cells by promoting apoptosis, inhibiting cell growth, or maintaining DNA

28
Q

what are the two categories the tumor suppressor proteins can be divided into

A

caretakers and gatekeepers

29
Q

which category of tumor suppressor proteins make sure that the genome remains stable through DNA repair?

A

caretakers

30
Q

which category of tumor suppressor proteins regulate cell growth by inhibiting cell cycle progression?

A

gatekeepers

31
Q

what tumor suppressor protein prevents the progression of the cell from G1 to S?

A

Retinoblastoma protein (Rb)

32
Q

when is Rb active?

A

resting cells remaining in G1

33
Q

what state is Rb in when it’s active?

A

hypophosphorylated (no phosphate attached)

34
Q

When the cell is ready to progress to S from G1, we will have an increased presence of what cyclins and CDK?

A

cyclin D
CDK 4
CDK6

35
Q

what enzyme adds more phosphate groups to Rb?

A

kinases

36
Q

what state is the Rb in when it is inactive?

A

hyperphosphorylated (many phosphates added)

37
Q

When Rb is inactive, it will cause it to dissociate from what protein?

A

E2F

38
Q

what does E2F do?

A

activate gene expression of cyclins E and A for progression into the S phase

39
Q

what tumor suppressor protein is a caretaker protein and called “the guardian of the genome”?

A

p53

40
Q

what is the purpose of p53?

A

ensures that DNA’s integrity is maintained

41
Q

If p53 notices any DNA damage, it arrests the cell in the ____ checkpoint and activates DNA repair mechanisms.

A

G1/S

42
Q

how does p53 arrest the cell?

A

CDK inhibitor p21

43
Q

If p53 recognizes that this DNA damage cannot be repaired, it activates apoptosis by inducing the transcription of the protein ___, which induces apoptosis.

A

BAX

44
Q

what is retinoblastoma?

A

childhood cancer that occurs due to a mutation in the Rb gene which is located on chromosome 13

45
Q

what can happen when tumor suppressor genes lose their function due to a mutation?

A

cause cancer

46
Q

what must happen for cancer to develop by having a mutated tumor suppressor gene?

A

both genes must have a loss of function

47
Q

why is retinoblastoma considered a familial cancer?

A

most children inherit one defective Rb allele from a parent who is a carrier

48
Q

what is the two hit hypothesis?

A
  • once the second Rb gene is mutated, they will develop this cancer
  • in order to develop cancers due to loss of function of a tumor suppressor gene, you must have two hits on the tumor suppressor gene (losing function in both genes)
49
Q

what are symptoms of retinoblastoma?

A

leukocoria (abnormal white retina appearance)
loss of vision
glaucoma
cross-eyed vision

50
Q

what is mitosis

A

process of cell division to create an identical daughter cell

51
Q

list the different phases of mitosis in order

A

prophase
prometaphase
metaphase
anaphase
telophase
cytokinesis

52
Q

which mitosis phase involves condensation of the replicated DNA into chromosomes?

A

prophase

53
Q

during prophase, because the DNA has duplicated in the S phase, each chromosome will consist of two sister chromatids that are joined together by a _______.

A

centromere

54
Q

during prophase, microtubules in the cytoskeleton aggregate around ________, which are the microtubule-organizing centers, which will be used as part of the ___________

A

centrosomes
mitotic spindle

55
Q

what is the start of prometaphase characterized by?

A

breakdown of the nuclear envelope where the spindle can then enter the nuclear area

56
Q

during prometaphase, what are the structure that begin to develop on the centromeres of the chromosomes?

A

kinetochores

57
Q

during prometaphase, what do the kinetochores attach to?

A

kinetochore microtubules

58
Q

what is metaphase characterized by?

A

where the chromosomes are lined up in the middle of the cell

59
Q

how are the alignment of chromosomes done during metaphase?

A

kinetochore microtubules and motor proteins

60
Q

what happens during anaphase?

A

chromosomes separate into chromatids that go to opposite poles of the cell

61
Q

which phase is faster, anaphase or metaphase?

A

anaphase

62
Q

what happens during telophase?

A

the separated sister chromatids arrive at the opposite poles and the kinetochore microtubules begin to disappear

63
Q

what happens during cytokinesis?

A

cells separate to make two new daughter cells

64
Q

what phase does cleavage occur?

A

cytokinesis

65
Q

how is a cleavage furrow created?

A

contractile ring of actin filaments, and it gradually becomes deeper and deeper until both sides reach the remaining mitotic spindle that is still present between the two new nuclei