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CC PART 1 Flashcards

1
Q

deci

A

1

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2
Q

centi

A

2

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3
Q

milli

A

3

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4
Q

micro

A

6

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5
Q

nano

A

9

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6
Q

pico

A

12

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7
Q

femto

A

15

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8
Q

base SI unit for the amount of a susbtance

A

mole

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9
Q

conc expressed as the amount of solute per 100 parts of solution

A

percent solution

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10
Q

moles per liter of solution

A

molarity

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11
Q

moles of solute per KG of solvent

A

molality

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12
Q

indication of relative concentration

A

dilution

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13
Q

formula of dilution

A

SOLUTE:TOTAL VOLUME
C1V1=C2V2

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14
Q

amount of something in PROPORTION to the amount of something else

A

ratio

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15
Q

pH formula

A

Henderson-Hasselbach Equation
ph=pKa+logsalt/acid

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16
Q

ratio of blood drawn and serum/plasma yielded

A

1:1

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17
Q

colligative properties (4)

A

Vapor pressure
boiling point
freezing point
osmotic pressure

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18
Q

vapor pressure

A

DECREASED BY 0.3 mmhg or torr

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19
Q

boiling point

A

INCREASED by 0.52C

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20
Q

Freezing point

A

DECREASED BY 1.86C

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21
Q

osmotic pressure

A

increased by 1.7x10to the 4th power

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22
Q

CLASS OF FIRE
water

A

A

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23
Q

CLASS OF FIRE
dry chemicals

A

A B C

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24
Q

CLASS OF FIRE
sodium potassiom

A

D

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25
Q

CLASS OF FIRE
cooking oil/ combustible oil

A

K

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26
Q

BEST type of Extinguisher

A

DRY CHEMICALS

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27
Q

CLASS OF FIRE
ordinary combustiles; wood. paper, cloth

A

A

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28
Q

CLASS OF FIRE
flammable liquids

A

B

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29
Q

CLASS OF FIRE
electrical equipment

A

C

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30
Q

CLASS OF FIRE
flammable metals

A

D

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31
Q

one of the most widely used cryogenic fluids in the lab

A

Liquid Nitrogen

32
Q

injury from repititive strain

A

ERGONOMIC HAZARD

33
Q

GRADES OF CHEMICALS (5)

A

analytic reagent
ultrapure
chemically pure
USP NF
Technical/Commercial

34
Q

GRADES OF CHEMICALS

very high purity
recommended for qualitative and quantitative analysis/most analytical lab procedures

A

analytic reagent

35
Q

GRADES OF CHEMICALS

highest purity
Used for AAS, molecular diag, immunoassays, HPLC

A

ultrapure

36
Q

GRADES OF CHEMICALS

melting point analysis

A

chemically pure

37
Q

GRADES OF CHEMICALS

not recommended for clinical labs

A

chemically pure

38
Q

GRADES OF CHEMICALS

not injurious to health.
used to manufacture drugs

A

US Pharmacopeia and National Formulary

39
Q

most freq. used reagtent in the lab

A

water

40
Q

Type (CLSI)

pure enough for most routine testing

A

Clinical laboratory reagent grade (CLRW)

41
Q

Type (CLSI)

replaces previously designated type I and II water

A

Clinical laboratory reagent grade (CLRW)

42
Q

Type (CLSI)

for applications that req. water of different purity

A

Special Reagent water(SRW)

43
Q

Type (CLSI)

replaces previously designated type III water

A

Autoclave and wash water

44
Q

GLASSWARE

high resistance to thermal shock and chemical attack.

A

borosilicate glass

45
Q

GLASSWARE

most commonly used

A

borosilicate glass

46
Q

GLASSWARE

6 times stronger tham borosilicate

A

Aluminosilicate glass

47
Q

GLASSWARE

Acid resistant
High thermal and heat shock resistance

A

vycor

48
Q

GLASSWARE

alkali resistant
poor heat resistant

A

Boron free/soft glass

49
Q

MOST BASIC PIPET

A

GLASS PIPET

50
Q

Calibration of TD pipets

A

distilled water

51
Q

Calibration of TC pipets

A

mercury

52
Q

Calibration of Class A pipets

A

do not require calibration

53
Q

routine cleaning/washing of glasswares (3)

A

-soaking in dilute bleach or 10% hypochlorite solution followed by drying in an oven.
- soaking in 20% nitric acid solution for 12-24 hours
-soaking in SULFURIC-ACID-DICHROMATE SOLUTION

54
Q

routine cleaning of glasswares for BACTERIOLOGIC GLASSWARE

A

soak in 2-4% cresol solution followed by autoclaving and thorough washing

55
Q

speed of centrifugation

A

rpm

56
Q

determined by tachometer or strobe light

A

rpm

57
Q

CENTRIFUGE

tubes are in horizontal when rotating and vertical in rest

A

horizontal head centrifuge (swinging bucket)

58
Q

CENTRIFUGE

recommended for serum separator tube

A

horizontal head centrifuge (swinging bucket)

59
Q

CENTRIFUGE

tubes are at fixed angle (25-40) when rotating, capable of higher speed

A

angle-head centrifuge

60
Q

CENTRIFUGE

HIGHEST SPEED
capable of 100,000 rpm.
for lipoprotein analysis

A

ultra centrifuge

61
Q

Calibration of centrifuge

A

quarterly or every 3 months

62
Q

checking of rpm

A

monthly

63
Q

disinfection of centrifuge

A

weekly

64
Q

cetrifuge that produces a monolayer of cells

A

cytocentrifuge

65
Q

reagents, diluents and samples are pumped sequentially through a system of continuous tubing

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

a.

66
Q

air bubbles at regular intervals serve as separating and cleaning media.

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

a

67
Q

parallel testing, batch analysis

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

a

68
Q

significant carry-over effect and wasteful use of reagents

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

a

69
Q

uses acceleration and deceleration of centrifuge rotor to transfer the reagents and sample from one chamber to another

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

b

70
Q

batch analysis

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

b

71
Q

separation of each sample and accompanying reagents in a separate container

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

c

72
Q

measures only the tests requested on a sample

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

c

73
Q

multiple-tests-one-sample-at-a-time

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

c

74
Q

random access capability and batch analysis capability

a. continuous flow analyzer
b. centrifugal analyzer
c. discrete analyzer

A

c

75
Q

can be packaged as lyophilized powder or tablet form that must be reconstituted with a buffer or reagent grade water

a. dry reagents
b. liquid reagents

A

a

76
Q

directly pipetted with a instrument and mixed with the sample

a. dry reagents
b. liquid reagents

A

b

CC (8 decks)

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