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Microbiology and Immunology Pracs > Case 50: An outbreak of infections in an intensive care ward > Flashcards

Case 50: An outbreak of infections in an intensive care ward Flashcards

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Biology 101 flashcards
1
Q

What is the Gram stain for pseudomonas aeruginosa?

A

Gram negative rods

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2
Q

What is the colonial morphology of Ps. aeruginosa on HBA?

A

No growin in AnO2

O2: green, slimy (beta haemolysis) - although it looks like alpha haemolysis because it’s green

Strict aerobe

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3
Q

What is the colonial morphology of Ps. aeruginosa on MAC?

A

Yellowish, slimy

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4
Q

What is the colonial morphology of Ps. aeruginosa on Cetrimide agar?

A

Light green, slimy

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5
Q

How does alpha haemolysis appear on HBA?

A

Greening

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6
Q

How does beta haemolysis appear on HBA?

A

Clearing

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7
Q

Why is HBA indication of alpha or beta haemolysis unreliable with ps. aeruginosa?

A

Because ps. aeruginosa is green anyway

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8
Q

Why is cetrimide agar used for identification of ps. aeruginosa?

A

Because it is a commonly used hospital disinfectant - ps. aeruginosa is very good at growing in a hostile environment

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9
Q

Which common antimicrobials is ps. aeruginosa resistant to in nosocomial infections?

A

All: piperacillin, ticarcillin and cefotaxime

Ticarcillin is ordinarily for pseudomonas

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10
Q

What is piperacillin?

A

Synthetic beta lactam designed to combat ps. aeruginosa

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11
Q

What is used in conjugation with ticarcillin to treat ps. aeruginosa?

A

Clavulanic acid

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12
Q

What is used in conjugation with piperacillin to treat ps. aeruginosa?

A

Tazobactam

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13
Q

Which antimicrobials is ps. aeruginosa susceptible to?

A

Piperacillin + tazobactam

Aztreonam

Ticarcillin + clavulanic acid (expensive)

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14
Q

What is tazobactam?

A

Beta lactamase inhibitor that targets ps. aeruginosa

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15
Q

What is the recommended empiric treatment for ps. aeruginosa?

A

Piperacillin and tazobactam

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16
Q

What 4 pieces of information are required to design an appropriate sterilisation process for a particular instrument?

A

Type and level of contamination

Rate of biocidal action of agent (D-value is time to reduce population 10-fold)

Level of sterility assurance

Can the equipment withstand sterilisation process

Microbiology and Immunology Pracs (26 decks)

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