carbohydrates Flashcards
general formula of carbohydrates?
Cₓ(H₂O)ᵧ
substances that make up carbohydrates
- carbon
- hydrogen
- oxygen
definition of monosaccharide
a carbohydrate that cannot be hydrolysed to a simpler carbohydrate
general formula of monosaccharide
(CH₂O)ₙ
C6H12O6
examples of monosaccharides
- glucose
- fructose
- galactose
definition of disaccharide
formed by a condesation reaction between two monosaccharides - one molecule of water is removed from the pair of monosaccharides
examples of dissacharides
- maltose - glucose + glucose
- sucrose - glucose + fructose
- lactose - glucose + galactose
bond formed between two monosaccharides / bond in disaccharide
glycosidic bond
what processes form and breaks glycosidic bonds?
forms - condensation (removes a water molecule)
breaks - hydrolysis (adds a water molecule)
definition of polysaccharide
polymer of monosaccharides
examples of polysaccharides
- starch - storage polysaccharide in plants
- glycogen - storage polysaccharide in humans
- cellulose - structural polysaccharide in plants
formula for starch
C6H12O6 (glucose) - H2O (condensation) =
(C6H10O5)
structure of starch
- polymer of glucose
- two components : amylose and amylopectin
- straight chain structure with helical shape
- several thousand glucose unit joined by glycosidic bonds
details of amylopectin
- compact with highly branched structure
- twice as many glucsose units as amylose.
functions of carbohydrates
1] source of energy
2] sucrose is a good transport sugar in the phloem of plants
* very soluble
* chemically unreactive
3] polysaccharides (starch and glycogen) are good storage molecules
* large and insoluble
* compact shapes
* easily hydrolysed into monosaccharides
4] cellulose is a good structural polysaccharide
* good tensile strength
* permeable
5] monosaccharides needed to synthesise:
* dissacharides and polysaccharides
* nucleic acids (RNA, DNA)
test for reducing sugars
Benedict’s Test
1. To 2cm³ of the test sample in a test tube, add an equal volume of Benedict’s solution
2. Mix well and place in a boiling water bath for 2 minutes
3. Observe any color change
present: turns green, yellow, brown, red
absent: remains blue
test for non reducing sugar
- To 2cm³ of the test sample in the test tube, add a few drops of HCl.
- Heat the mixture for 2 mins in a hot water bath
- After 2 minutes, neutralise the acid by adding sodium hydrogen carbonate until effervescence stops.
- Carry out Benedict’s test
- Test will now turn out to be positive as non reducing sugar is broken down into reducing sugars.
test for starch
- Add a few drops of iodine solution to the test sample in a test tube or white tile
- observe any color change.
present: dark blue
absent: remains brown.