Bordetella, Pseudomonas

Question 1

Bordetella pertussis (6):

Answer 1

• Capable of invading respiratory tract.
• Recurrent, violent cough that can last up to 6 weeks.
• Encapsulated coccobacillus.
• Non-fermentative.
• Gram negative rods.
• Nutritionally fastidious.

Question 2

Bordetella pertussis virulence factors (7):

Answer 2

Adhesins:
- Filamentous hemagglutinin (FHA).
- Pili and pertactin.
Toxins:
- Pertussis toxin.
- Invasive adenylate cyclase.
- Dermonecrotic toxin (lethal toxin).
- Tracheal cytotoxin.
- LPS - Lipid A and X

Question 3

Pertussis diagnosis methods (2):

Answer 3

• Direct fluorescent antibody (DFA).

- PCR

Question 4

Pseudomonas aeruginosa (7):

Answer 4

• Gram negative rod.
• Non-fermenter.
• Motile with flagella.
• Aerobic.
• Simple growth requirement.
• Ubiquitous.
• Green and blue pigments.

Question 5

Pseudomonas aeruginosa virulence factors (6):

Answer 5

• Exotoxin A.
• Exoenzyme S.
• Elastase.
• Phospholipase C/Heat-stable phospholipase.
• Alkaline phosphotase.
• Alginate.

Question 6

Exotoxin A:

Answer 6

ADP-ribosylation of EFII.

Question 7

Exoenzyme S:

Answer 7

ADP-rybosylates several proteins. Type III secretion system.

Question 8

Phospholipase:

Answer 8

Hydrolysis of phospholipids leading to tissue damage.

Question 9

Alkaline phosphotase:

Answer 9

Proteolysis leading to tissue damage.

Question 10

Alginate:

Answer 10

Promotes adherence to respiratory epithelium, interferes with effective phagocytosis and may be immunostimulatory.

Question 11

Pseudomonas folliculitis:

Answer 11

Resulting from immersion in contaminated water such as hot tubs, whirlpools, swimming pools.

Question 12

Three pathogens can colonize the CF lung:

Answer 12

• S. aureus.
• H. influenzae.
• P. aeruginosa.