BOC- Bloodbank

Question 1

The minimum hemoglobin concentration in a fingerstick from a male blood donor is:

Answer 1

12.5 g/dL

Question 2

A cause for permanent deferral of blood donation is:

a) diabetes
b) residence in an endemic malaria region
c) history of jaundice of uncertain cause
d) history of therrapeutic rabies vaccine

Answer 2

c) history of jaundice of uncertain cause

Question 3

Which of the following prospective donors would be accepted for donation?

a) 32 year old women who received a transfusion in a complicated delivery 5 months previously?
b) 19 yr old sailor who has been stateside for 9 months and stopped taking his anti-malarial meds 9 months previously?
c) 22 yr old college student who has a temperature of 99.2 and states that he feels well, but is nervous about donating
d) 45 yr old woman who has just recovered from a bladder infection and is still taking antibiotics?

Answer 3

c) 22 year old with fever

Question 4

Which of the following constitutes a permanent rejection status of a donor?

a) a tattoo 5 months previously
b) recent close contact with a patient with viral hepatitus
c) 2 units of blood transfused 4 months previously
d) confirmed positive test for HBsAg 10 years previously

Answer 4

d) confirmed positive test for HBsAg

Question 5

According to AABB standards, which of the following donors may be accepted as a blood donor?

a) traveled to an area endemic for malaria 9 months previously
b) spontaneous abortion at 2 months of pregnancy, 3 months previously
c) resides with a known hepatitis patient
d) received a blood transfusion 22 weeks previously

Answer 5

b) spontaneous abortion at 2 months of pregnancy, 3 months previously

Question 6

For a female blood donor the Hct must be what?

Answer 6

> 38%

Question 7

For apheresis donors who donate platelets more frequently than every 4 wks, a platelet count must be performed prior to the procedure and be at least?

Answer 7

150 X 10^3/uL

Question 8

Prior to blood donation, the intended venipuncture site must be cleaned with a scrub solution containing ?

Answer 8

PVP iodine complex

Question 9

All donor blood testing must include?

Answer 9

serological test for syphilis

Question 10

During the preparation of Platelet Concentrates from Whole Blood, the blood should be?

Answer 10

cooled towards 20-24 C

Question 11

The most common cause of posttransfusion hepatitis can be detected in donors by testing for?

Answer 11

HBsAg

Question 12

The Western blot is a confirmatory test for the presence of?

Answer 12

anti-HIV 1

Question 13

The test that is currently used to detect donors who are infected with AIDS virus is?

Answer 13

anti-HIV 1,2

Question 14

A commonly used screening method for anti-HIV-1 detection is?

Answer 14

enzyme -labeled immunosorbent assay (ELISA)

Question 15

Rejuvenation of a unit of RBCs is a method used to?

Answer 15

restore 2,3-DPG and ATP to normal levels

Question 16

A unit of packed cells is split into 2 aliquots under closed sterile conditions at 8am. The expiration time for each aliquot is now?

Answer 16

the original date of the unsplit unit.

* a sterile docking device allows entry into the unit without affecting the expiration date of the unit.

Question 17

A unit of RBCs expiring in 35 days is split into 5 small aliquots using a sterile pediatric quad set and a sterile connecting device. Each aliquot must be labeled with an expiration date of?

Answer 17

35 days.

Question 18

When platelets are stored on a rotator set on an open bench top, the ambient air temp must be recorded?

Answer 18

every 4 hours if they are not in a unit with automated temp recording

Question 19

What is the proper temperature for storage of Fresh Frozen Plasma (FFP)?

Answer 19

-20

Question 20

What is the optimum storage temperature for Red Blood Cells, Frozen?

Answer 20

at -65 or lower with 40% glycerol

Question 21

A unit of RBC is issued at 0900 but returned at 0910 unused can it be put back into the refrigerator?

Answer 21

yes, as: 1) long as the container was not gone into

          2) there is at least 1 sealed segment attached 
          3) visual inspection of the unit is satisfactory
          4) unit has been maintained at an acceptable temp

Question 22

The optimum storage temperature for RBCs?

Answer 22

1-6 Celsius

Question 23

If the seal is entered on a unit of RBCs stored at 1-6 C, what is the maximum allowable period?

Answer 23

24 hours ( expiration on open system RBCs is 24 hours)

Question 24

The optimum storage temperature for cryoprecipitated AHF is?

Answer 24

-18 Celsius or lower

Question 25

Cryoprecipitated AHF must be transfused within what period of time following thawing and pooling?

Answer 25

within 4 hours of thawing

Question 26

Platelets prepared in a polyolefin type container, stored at 22-24 C in 50 mL of plasma, and gently agitated can be used for up to?

Answer 26

5 days

Question 27

The optimum storage temperature for platelets?

Answer 27

22 C

Question 28

According to AABB standards, Fresh Frozen Plasma must be infused within what period of time following thawing?

Answer 28

within 24 hours | * should be stored at 1-6 no more than 24 hours after thawing)

Question 29

Cryoprecipitated AHF, if maintained in the frozen state at -18 C or below, has a shelf life of?

Answer 29

12 months

Question 30

Once thawed, Fresh Frozen Plasma must be transfused within

Answer 30

24 hours

Question 31

An important determinant of platelet viability following storage is?

Answer 31

plasma pH

Question 32

In the liquid state, the concentration of 2,3-diphosphoglycerate (2,3-DPG) decreases in a unit of?

Answer 32

RBCs

Question 33

In the liquid state, plasma must be stored at ?

Answer 33

1-6 C

Question 34

Cryoprecipitated AHF is indicated for?

Answer 34

fibrinogen deficiencies

Question 35

Which apheresis platelets product should be irradiated?

Answer 35

a directed donation given by a mother for her son

Question 36

Irradiation of a unit of RBCs is done to prevent the replication of donor?

Answer 36

lymphocytes

Question 37

Plastic bag overwraps are recommended when thawing units of FFP in 37 C degree water baths because they prevent what?

Answer 37

the entry ports from becoming contaminated with water

Question 38

What bloods components must be prepared within 8 hours after phlebotomy?

Answer 38

Fresh Frozen Plasma

Question 39

Cryoprecipitated AHF contains how many units of factor VIII?

Answer 39

80 units of Factor VIII

Question 40

``` Which blood components contain the most Factor VIII concentration relative to volume? a) Single-Donor Plasma b) Cryoprecipitated AHF c) Fresh Frozen Plasma D) Platelets ```

Answer 40

b) cryoprecipitated AHF contains at least 80IU of Factor VIII concentrated in about 10mL of plasma

Question 41

The most effective component to treat a patient with fibrinogen deficiency is?

Answer 41

Cryoprecipitate is indicated as a source of fibrinogen for hypofibrinogenemia

Question 42

A blood component prepared by thawing Fresh Frozen Plasma at refrigerator temperature and removing the fluid portion is?

Answer 42

Cryoprecipitate | *is the fraction of plasma protein that precipitate when FFP is slowly thawed at 1-6 degrees C.

Question 43

Upon inspection, a unit of platelets is noted to have visible clots, but otherwise appears normal. What should the technologists do?

Answer 43

Quarantine for a Gram stain and culture. Clots may indicate contamination.

Question 44

According to AABB Standards, at least 90% of all Apheresis Platelets units tested shall contain a minimum of how many platelets?

Answer 44

at least 3.0 x 10^11 platelets

Question 45

According to AABB Standards, Platelets prepared from whole blood shall have at least?

Answer 45

at least 5.5 x 10^10 platelets

Question 46

What is the proper procedure for preparation of platelets from whole blood?

Answer 46

light spin followed by a hard spin

Question 47

According to AABB standards, what is the minimum pH required for Platelets at the end of the storage period?

Answer 47

6.2

Question 48

According to AABB standards, Platelets must be?

Answer 48

platelets must be agitated gently if stored at room temperature

Question 49

A unit of Whole Blood-derived (random donor) Platelets should contain at least?

Answer 49

5.5 x 10^10 platelets

Question 50

Platelets prepared by apheresis should contain at least?

Answer 50

3 x 10^11 platelets, be stored at 22 degrees C with continuous agitation and have a pH of 6.2 or higher when tested at the end of storage

Question 51

Leukocyte- Reduced RBCs are ordered for a newly diagnosed bone marrow candidate. What is the best way to prepare this product?

Answer 51

Transfuse through a Log^3 leukocyte-removing filter

Question 52

What blood component should be used to prevent HLA alloimmunization of the recipient?

Answer 52

Leukocyte-Reduced RBCs

Question 53

A father donating Platelets for his son is connected to a continuous flow machine, which uses the principle of centrifugation to separate Platelets from Whole Blood. As the Platelets are harvested, all other remaining elements are returned to the donor. What is this method of Platelet collection?

Answer 53

apheresis

Question 54

To qualify as a donor for autologous transfusion a patient's hemoglobin should be at least?

Answer 54

11 g/dL

Question 55

What is/are the minimum pretransfusion testing requirement(s) for autologous donations collected and transfused by the same facility?

Answer 55

ABO and Rh typing only

Question 56

In a quality assurance program, Cryoprecipitated AHF must contain a minimum of how many internal units of Factor VIII?

Answer 56

80

Question 57

An assay of plasma from a bag of Cryoprecipitated AHF yields a [9 international units] of factor VIII per mL of Cryoprecipitated AHF. If the volume is 9 mL, what is the Factor VIII content of the bag in IU?

Answer 57

81 *To determine the total IU of Factor VIII per bag of cryoprecipitate, multiply the assayed

Question 58

A blood donor has the genotype of hh, AB. What is his red blood cell phenotype?

Answer 58

O

Question 59

An individual has been sensitized to the k antigen and has produced anti-k. What is her most probable Kell system genotype?

Answer 59

KK

Question 60

Given the following typing results, what is this donor's racial ethnicity? Le(a-b-);Fy(a-b-); Js(a+b+)

Answer 60

African American. Fy(a-b-) individuals are rare with all populations other than the individual of African descent. 68% of African Americans are Fy(a-b-).