BOC- Bloodbank Flashcards

1
Q

The minimum hemoglobin concentration in a fingerstick from a male blood donor is:

A

12.5 g/dL

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2
Q

A cause for permanent deferral of blood donation is:

a) diabetes
b) residence in an endemic malaria region
c) history of jaundice of uncertain cause
d) history of therrapeutic rabies vaccine

A

c) history of jaundice of uncertain cause

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3
Q

Which of the following prospective donors would be accepted for donation?

a) 32 year old women who received a transfusion in a complicated delivery 5 months previously?
b) 19 yr old sailor who has been stateside for 9 months and stopped taking his anti-malarial meds 9 months previously?
c) 22 yr old college student who has a temperature of 99.2 and states that he feels well, but is nervous about donating
d) 45 yr old woman who has just recovered from a bladder infection and is still taking antibiotics?

A

c) 22 year old with fever

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4
Q

Which of the following constitutes a permanent rejection status of a donor?

a) a tattoo 5 months previously
b) recent close contact with a patient with viral hepatitus
c) 2 units of blood transfused 4 months previously
d) confirmed positive test for HBsAg 10 years previously

A

d) confirmed positive test for HBsAg

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5
Q

According to AABB standards, which of the following donors may be accepted as a blood donor?

a) traveled to an area endemic for malaria 9 months previously
b) spontaneous abortion at 2 months of pregnancy, 3 months previously
c) resides with a known hepatitis patient
d) received a blood transfusion 22 weeks previously

A

b) spontaneous abortion at 2 months of pregnancy, 3 months previously

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6
Q

For a female blood donor the Hct must be what?

A

> 38%

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7
Q

For apheresis donors who donate platelets more frequently than every 4 wks, a platelet count must be performed prior to the procedure and be at least?

A

150 X 10^3/uL

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8
Q

Prior to blood donation, the intended venipuncture site must be cleaned with a scrub solution containing ?

A

PVP iodine complex

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9
Q

All donor blood testing must include?

A

serological test for syphilis

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10
Q

During the preparation of Platelet Concentrates from Whole Blood, the blood should be?

A

cooled towards 20-24 C

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11
Q

The most common cause of posttransfusion hepatitis can be detected in donors by testing for?

A

HBsAg

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12
Q

The Western blot is a confirmatory test for the presence of?

A

anti-HIV 1

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13
Q

The test that is currently used to detect donors who are infected with AIDS virus is?

A

anti-HIV 1,2

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14
Q

A commonly used screening method for anti-HIV-1 detection is?

A

enzyme -labeled immunosorbent assay (ELISA)

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15
Q

Rejuvenation of a unit of RBCs is a method used to?

A

restore 2,3-DPG and ATP to normal levels

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16
Q

A unit of packed cells is split into 2 aliquots under closed sterile conditions at 8am. The expiration time for each aliquot is now?

A

the original date of the unsplit unit.

* a sterile docking device allows entry into the unit without affecting the expiration date of the unit.

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17
Q

A unit of RBCs expiring in 35 days is split into 5 small aliquots using a sterile pediatric quad set and a sterile connecting device. Each aliquot must be labeled with an expiration date of?

A

35 days.

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18
Q

When platelets are stored on a rotator set on an open bench top, the ambient air temp must be recorded?

A

every 4 hours if they are not in a unit with automated temp recording

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19
Q

What is the proper temperature for storage of Fresh Frozen Plasma (FFP)?

A

-20

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20
Q

What is the optimum storage temperature for Red Blood Cells, Frozen?

A

at -65 or lower with 40% glycerol

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21
Q

A unit of RBC is issued at 0900 but returned at 0910 unused can it be put back into the refrigerator?

A

yes, as: 1) long as the container was not gone into

          2) there is at least 1 sealed segment attached 
          3) visual inspection of the unit is satisfactory
          4) unit has been maintained at an acceptable temp
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22
Q

The optimum storage temperature for RBCs?

A

1-6 Celsius

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23
Q

If the seal is entered on a unit of RBCs stored at 1-6 C, what is the maximum allowable period?

A

24 hours ( expiration on open system RBCs is 24 hours)

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24
Q

The optimum storage temperature for cryoprecipitated AHF is?

A

-18 Celsius or lower

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25
Q

Cryoprecipitated AHF must be transfused within what period of time following thawing and pooling?

A

within 4 hours of thawing

26
Q

Platelets prepared in a polyolefin type container, stored at 22-24 C in 50 mL of plasma, and gently agitated can be used for up to?

A

5 days

27
Q

The optimum storage temperature for platelets?

A

22 C

28
Q

According to AABB standards, Fresh Frozen Plasma must be infused within what period of time following thawing?

A

within 24 hours

* should be stored at 1-6 no more than 24 hours after thawing)

29
Q

Cryoprecipitated AHF, if maintained in the frozen state at -18 C or below, has a shelf life of?

A

12 months

30
Q

Once thawed, Fresh Frozen Plasma must be transfused within

A

24 hours

31
Q

An important determinant of platelet viability following storage is?

A

plasma pH

32
Q

In the liquid state, the concentration of 2,3-diphosphoglycerate (2,3-DPG) decreases in a unit of?

A

RBCs

33
Q

In the liquid state, plasma must be stored at ?

A

1-6 C

34
Q

Cryoprecipitated AHF is indicated for?

A

fibrinogen deficiencies

35
Q

Which apheresis platelets product should be irradiated?

A

a directed donation given by a mother for her son

36
Q

Irradiation of a unit of RBCs is done to prevent the replication of donor?

A

lymphocytes

37
Q

Plastic bag overwraps are recommended when thawing units of FFP in 37 C degree water baths because they prevent what?

A

the entry ports from becoming contaminated with water

38
Q

What bloods components must be prepared within 8 hours after phlebotomy?

A

Fresh Frozen Plasma

39
Q

Cryoprecipitated AHF contains how many units of factor VIII?

A

80 units of Factor VIII

40
Q
Which blood components contain the most Factor VIII concentration relative to volume?
a) Single-Donor Plasma
b) Cryoprecipitated AHF
c) Fresh Frozen Plasma
D) Platelets
A

b) cryoprecipitated AHF contains at least 80IU of Factor VIII concentrated in about 10mL of plasma

41
Q

The most effective component to treat a patient with fibrinogen deficiency is?

A

Cryoprecipitate is indicated as a source of fibrinogen for hypofibrinogenemia

42
Q

A blood component prepared by thawing Fresh Frozen Plasma at refrigerator temperature and removing the fluid portion is?

A

Cryoprecipitate

*is the fraction of plasma protein that precipitate when FFP is slowly thawed at 1-6 degrees C.

43
Q

Upon inspection, a unit of platelets is noted to have visible clots, but otherwise appears normal. What should the technologists do?

A

Quarantine for a Gram stain and culture. Clots may indicate contamination.

44
Q

According to AABB Standards, at least 90% of all Apheresis Platelets units tested shall contain a minimum of how many platelets?

A

at least 3.0 x 10^11 platelets

45
Q

According to AABB Standards, Platelets prepared from whole blood shall have at least?

A

at least 5.5 x 10^10 platelets

46
Q

What is the proper procedure for preparation of platelets from whole blood?

A

light spin followed by a hard spin

47
Q

According to AABB standards, what is the minimum pH required for Platelets at the end of the storage period?

A

6.2

48
Q

According to AABB standards, Platelets must be?

A

platelets must be agitated gently if stored at room temperature

49
Q

A unit of Whole Blood-derived (random donor) Platelets should contain at least?

A

5.5 x 10^10 platelets

50
Q

Platelets prepared by apheresis should contain at least?

A

3 x 10^11 platelets, be stored at 22 degrees C with continuous agitation and have a pH of 6.2 or higher when tested at the end of storage

51
Q

Leukocyte- Reduced RBCs are ordered for a newly diagnosed bone marrow candidate. What is the best way to prepare this product?

A

Transfuse through a Log^3 leukocyte-removing filter

52
Q

What blood component should be used to prevent HLA alloimmunization of the recipient?

A

Leukocyte-Reduced RBCs

53
Q

A father donating Platelets for his son is connected to a continuous flow machine, which uses the principle of centrifugation to separate Platelets from Whole Blood. As the Platelets are harvested, all other remaining elements are returned to the donor. What is this method of Platelet collection?

A

apheresis

54
Q

To qualify as a donor for autologous transfusion a patient’s hemoglobin should be at least?

A

11 g/dL

55
Q

What is/are the minimum pretransfusion testing requirement(s) for autologous donations collected and transfused by the same facility?

A

ABO and Rh typing only

56
Q

In a quality assurance program, Cryoprecipitated AHF must contain a minimum of how many internal units of Factor VIII?

A

80

57
Q

An assay of plasma from a bag of Cryoprecipitated AHF yields a [9 international units] of factor VIII per mL of Cryoprecipitated AHF. If the volume is 9 mL, what is the Factor VIII content of the bag in IU?

A

81

*To determine the total IU of Factor VIII per bag of cryoprecipitate, multiply the assayed

58
Q

A blood donor has the genotype of hh, AB. What is his red blood cell phenotype?

A

O

59
Q

An individual has been sensitized to the k antigen and has produced anti-k. What is her most probable Kell system genotype?

A

KK

60
Q

Given the following typing results, what is this donor’s racial ethnicity?
Le(a-b-);Fy(a-b-); Js(a+b+)

A

African American. Fy(a-b-) individuals are rare with all populations other than the individual of African descent. 68% of African Americans are Fy(a-b-).