Blood Smear (lab) Flashcards
Thin film/smear is used for diagnosing the
Anemia, leukemia, platelet disorder and abnormalities
Observing blood cell morphology and anomalies as well as to identify malari species
Thin film/smear
For rapid diagnosis of the presence of blood parasites like Malaria and Trypanosomes
Thick film/smear
Inc the chances of detecting light parasitic infections and dec the time needed for reliable diagnosis
Thick film/smear
Prelab activities
Prepare the necessary materials used
Be sure that laboratory is clean
Collected EDTA blood specimen
Members of this group of stains:
Wright's stain Leishman's stain Giemsa's stain Jenner-Giemsa stain May-Grunwald stain
Components of wright’s stain
Eosin and methylene blue
Fixative of blood film of wright’s stain
Methyl alcohol
Sete the desired pH for Wright’s stain
Phosphate buffer
Recommended for blood and bone marrow of Wright’s stain
pH 6.8
Recommended for malarial parasites for wright’s stain
pH 7.2
Areas where the RBCs are not overlapping but are just touching each other.
Feathery edge
pH of the PO4 buffer is too acidic in RBC
Bright red to red
pH of the PO4 buffer is too acidic in WBCs nuclei
Pale blue to colorless
pH of the PO4 buffer is too basic in RBCs
Deep blue