Blood bank automation Flashcards
Immediate spin
Do Blood typing
RH typing
or anything with a phenotype
Gold standard for testing in BB
tube testing
and your are the final say in results
employment, salary, electricity, machines=
Operational cost
Blood blank automation allows for more what and fewer what
more efficiency and fewer techs
Automation allows for increased
turn around time
you have to show that new machines match
current machines in efficiency
Kids picked up way faster in what
Solid phase then in tube and gel phase
Random access mode
Anytime you have a new specimen it can start up and do testing.
Simultaneous multiple analyze
Do multiple test at once. Blood typing vs ITE
Benchtop in most
Hospitals
Floor top in most
Reference labs
Liquid=
How much serum, plamsa, in tube. = short sample.
Automation doesn’t require as
Much of a sample therefore you can use left over sample for tube tests.
If you have a
STAT specimen it stops the regular samples and allows you to put in stat sample
Stand alone system
Still do pipetting/ Interpretation but the machine can incubate, wash only
there can be a bead
Bead at the bottom of bottle to spin at bottom in hemagglutination
The machine will
Interpretation of results
MLS has to
Verify what the machine states before results are sent out.
BIO-RAD
Has hemaagglutination test.
Erytype S test
Pre-coated with Anti-sera and immunor core added antisera in well
SPRCA assays will put
RBCs on bottom of wells so dont have to use RBC reagents but keeping humidity is important.
increased Humidity= messed up results.
LIST
ADD two drops contains proteins indicators to make sure sample was added to test well
Wash step
Washes away proteins unbound in test well
BB automation will not take all the
Sample
BIO-RAD solid serum II
Anti-b screen
Add different types of MLB-2 List
Cell in BIO rad are only good for
30 days
Provue instrument
Adding in all RBC this one is + benefit but dont have room for a new panel= take up entire reagents on instrument= downside= cant run continuously if needing to do two test such as Antibody ID test
Ortho has
Has probe for reagents and tubes
Echo has one probe
probe for reagent and tube
can only do one thing at a time
Testing
Random
Simultaneous multiple analyzers
Extensive test menu
Automated reader
Precise pipetting
Sampling handling
Clot detection
Liquid detection
Barcode reader
Closed tube sampling
Precise sampling
Accepts multiple samples
Data handling
Flexible software
Flags discrepancies
Lab information system interface
Automatic patient updates to lab information system
When you select automation you have to
Select vendor but first you have to determine vendor
Ortho only does
GEL
Imicore only does
Solid phase
Bio rad does
Both
TANGO has
Infinity ( BIO-RAD)
- combines Erytype S and solidscreen
Capture system has
Immuncor
- Microtiter plates accomondate SPRCA
- Hemagglutination testing.
In hemaaalutination assays what are used
Bar coded samples and reagents
Plates are automatically read using a camera
SPRCA procedure
1.) add 2 drops of LISS to precoated well
2.) Add 1 drop of patient sample
3.) incubate
4.) Wash
5.) Add one drop of indicator cells
6.) Centrifuge the test strip
7.) Read reactions
Immucor lumena has how many
Specimens
Reagents
Strips
20 specimens
16 reagents
32 strips
Ortho provue
has how many
Specimens
Reagents
Diluents
48 specimens
16 reagents
12 diluents
Immucor Lumena has how many barcode readers
2
Immucor lumena has how many probes
one
Ortho has 24
position capacity card blocks
Immucor has what reagents
LIST, ABO, and Indicator cells.
Provue you add in ( gel automation)
all of the RBCs so if you have a positive you dont have room to do another panel because it will take up all the reagents on the instruments
that is a down side
cant continously run to do antibody ID.
Solid phase allows you to ( echo)
to not have to add more reagents.
- all I have to do is put on cuvette that has wells that are coated with Red cells
Each strip is one test on for what test on what machine
Lumena Immucor
if doing blood types then one strip
If doing a antibody ID on the Immuncor then
you can use half a strip. so you can do two people with one strip
If doing a panel then you can use on Immucor
two strips
Cards are
cheap so you can load up cards
Ortho
1 camera for
Immucor
3 camera for
Ortho
Solid phase
Lumena
Gel
Ortho
