BIS 2A Flashcards
CH3
Methyl, nonpolar
OH
Hydroxyl, polar
COOH
Carboxylic group, polar
NH3
Amino group, polar
PO4-
Phosphate group, polar
Phosphorylation
Adding of phosphate groups, endergonic
Dephosphorylation
Splitting phosphate groups, exergonic
Phosphoanhydride bond
Bond between the two phosphate groups
Anaerobic
Does not require oxygen
Aerobic
Requires oxygen
Where does glycolysis occur?
Cytoplasm
Is glycolysis aerobic or anaerobic?
Anaerobic
What happens in a coupled reaction?
The enzyme allows a favorable reaction to occur, but an unfavorable reaction occurs simultaneously, because the activation energy was too high
Substrate level phosphorylation
Phosphate is donated by a high energy carbon compound
Main purpose of fermentation…
Replenish NAD+ levels
Two different types of fermentation:
Lactic acid and ethanol
What is pyruvate and what is it used for in fermentation?
Pyruvate is an electron acceptor used in fermentation to generate lactate and oxidize NADH to NAD+
What happens to lactate in fermentation?
Secreted from cell as waste
Is fermentation anaerobic or aerobic?
Anaerobic
What process is pyruvate oxidized?
TCA Cycle
Besides fermentation, where else can we recycle NADH back to NAD+?
Electron Transport Chain
At the end of glycolysis, where are the pyruvate molecules transported to?
Mitochondria
Where does respiration and electron transport chain occur in eukaryotes
Mitochondria
What is the terminal electron acceptor for humans?
O2
In glycolysis, for every molecule that is metabolized, ___ molecules of pyruvate are produced
2
Where does the TCA Cycle occur?
Matrix of mitochondria
In the TCA cycle, one acetyl CoA will yield…
One ATP/GTP, 3 NADH, 1 FADH2
What happens to CO2 in the TCA Cycle?
It is released as gas from breathing
What is a Metabolic Intermediate
Compound that is produced by a reaction which then acts as a substrate for the next reaction
What is the reductive TCA Cycle used for?
Construct glucose and other carbon containing molecules for CO2. ATP and NADH will be inputs since it is in reverse
What does the ETC produce?
Proton gradient
What is the proton gradient used for?
Running ATP synthase
A more negative reduction potential means…
The less likely the compound will accept electrons. Stronger electron donor
Oxidized compounds will have a higher…
Reduction potential
O2 is a very strong…
Oxidizing agent (Will become reduced)
+ delta G knot prime means what for delta E knot prime
It will be negative and vice versa
The oxidant is…
the compound getting electrons
The reductant is…
the compound losing electrons
What are the two major recycable energy carriers?
NAD+ and FAD(2+)
NAD+ is used as a reactant in …
While NADH is used as a reactant in…
Glycolysis, TCA Cycle
ETC, Fermentation
More CH bonds mean…
More reduced
ETC occurs in…
The inner mitochondrial matrix
What happens in respiration?
A series of redox reactions that transfer electrons to the terminal electron acceptor
The electrons passing through the ETC gradually lose…
Potential energy
Generating ATP from ADP is called…
Oxidative phosphorylation (Redox reaction and phosphorylation)
What does the ETC begin with?
Donation of electrons from NADH and FADH2
What is aerobic respiration?
Process of using oxygen as a terminal electron acceptor in an electron transport chain
The endergonic formation of a proton gradient is powered by…
the exergonic redox reactions
What is chemiosmosis used for?
Creating 90% of the ATP during aerobic glucose catabolism
What are the fates of an excited electron?
- Go back to ground state (fluoresced)
- Transferred by resonance
- Transferred to an electron acceptor (Photochemical reaction)
Photophosphorylation
Process of transferring light energy into ATP
What is special about green sulfur bacteria
Can perform photosynthesis using low-energy infrared photons becaause they live in the absence of light
In green sulfur bacteria photosynthesis, the electrons are cyclic/non-cyclic
Cyclic
In non-cyclic photophosphorylation, electrons end up onto…
NADPH after moving through ETC
NADPH in non-cyclic photophosphorylation will be used for…
Carbon fixation
Green sulfur bacteria evolved to utilize ___ as an electron donor, while green plants cannot
H2S
What is the overall function of light dependent reactions of oxygenic photophosphorylation?
Transform solar energy into chemical compounds in the form of NADPH and ATP
The energy from light dependent reactions is used for
reactants for the light independent reactions
Photosystem I and II are found in…
the thylakoid membrane
What happens in the Calvin Cycle?
The ATP will be input and the electron is deposited onto CO2 for long-term storage in the form of a carbohydrate
Calvin Cycle takes place in the…
Stroma
What is special about the reaction center?
Close to an oxidizing agent to undergo oxidation where the light energy is transformed to stable state
Z Scheme
PSII (P680) –> ETC –> P700ox –> ETC –> donated to NADP+
Along the way, proton gradient formed
P680ox replaces its electron by…
Taking an electron from water
What happens to water molecules in PSII?
Split to create O2. O2 is then released into the atmosphere
In photosynthesis, the plant cells use the ATP and NADPH formed during photophosphorylation to…
Reduce CO2 to sugar
What are autotrophs
Organisms that can obtained their carbon from an inorganic source
What are heterotrophs
Organisms that requrie organic carbon
Calvin Cycle leads to the reduction of
CO2 to G3P
What are the three stages of the Calvin Cycle
- Carbon fixation
- Redcution of 3-PGA
- Regeneration of RuBP
Amino acids are the…
monomers that make up proteins
What is the core structure of every amino acid
Alpha carbon bonded to an amino group, caboxyl group, a hydrogen atom, and R group
What is the backbone of an amino acid
N-C-C
N terminus denotes…
The beginning of the molecule and the amino group
C terminus denotes…
The end of the molecule and the carboxyl group
Where are peptide bonds formed
When two amino acids bond. Covalent bond
What kind of reaction occurs when a peptide bond is formed
Condensation/hydrogen synthesis
What bonds when a peptide bond is formed
The carboxyl group of one amino acid and the amino group of the other amino acid
What are the most common shapes in the secondary structure
alpha helix and beta sheets
Secondary structures are held together by…
hydrogen bonds from the backbone
What is the tertiary structure?
3-D structure of polypeptide
What creates the tertiary structure?
Interactions among R groups
What kind of bonds are found in tertiary structures?
Hydrogen bonds, hydrophobic interactions, ionic bonds, disulfide bridge (covalent bond)
When is a disulfide bridge created?
When two cysteine side chains interact
What kind of proteins can refold?
Small proteins
Quaternary structure
Weak interactions between multiple tertiary structures to act as one unit
When does denaturation occur
A proteins changes in temperature, pH, or exposure to chemicals and loses its shape
What are enzymes?
Catalysts that are made of proteins often with non-protein cofactors
Enzymes have an active site providing a unique chemical environment for…
Substrates
How are enzymes regulated
Inhibition and activation
Noncompetitive inhibitors are…
allosteric
The chemical reactants to which an enzyme binds are the enzyme’s…
substrates
The substrate binds onto the enzyme’s…
Active site
What is competitive inhibition?
When an inhibitor similar enough to a substrate binds to the active site to block the substrate from binding
What happens in noncompeititve inhibition?
The inhibitor molecule binds to a location that is not the active site. The binding alters the shape of the enzyme so it doesn’t bind effectively. Also called allosteric inhibition
Competitive inhibitors affect the __ but not the __ where noncompetitive inhibitors affect the ___
Initial rate; maximal rate; maximal rate
What are allosteric activators?
Bind to locations away from the active site, inducing a changw to increase chances of substrate binding to active site
What do cofactors do?
Bind to molecules to promote optimal function
What are prokaryotes?
Single celled organisms that do not have a distinct nucleus with a membrane or other organelles
Prokaryotes are composed of two distinct groups of organisms:
Bacteria and archaea
Bacteria and archaea are ciritcal for…
recycling nutrients essential for creating new biomolecules and the evolution of new ecosystems
What is a stromatolite?
Sedimentary structure formed when minerals precipitate out of water
What began the oxygenation of the atmosphere?
Cyanobacteria
What is a cell wall?
A protective structure that allows organisms to survive in extreme conditions
What are the three common shapes of bacteria and archaea?
Cocci (spherical), bacilli (rod-shaped), and spirlli (spiral-shaped)
Which aqueous compartment does ADP synthase occur?
Stroma
The plastid’s inner membrane and thylakoid membrane are resistant to…
the diffusion of protons
Water splitting occurs in the…
lumen of the thylakoids
In water splitting, you are generating
protons and electrons. Contibutes to proton gradient
Which side of the mitochondrial membrane would you make water?
On the matrix side
Integral proteins are embedded…
across the plane of the membrane
Peripheral proteins are attachded…
to the inner or outer surface
Oxidation of water by P680 is endergonic/exergonic?
exergonic
How are the 3 domains of life related?
A bacterium and an archean became symbionts; some of their descendants later evolved into eukaryotes
Which strategy for capturing energy came last?
Aerobic respiration
What might provide the energy required to concentrate the sugar lactose within a cell?
The coupled hydrolysis of ATP to ADP + Pi
What happens in G1 phase?
Cell growth
What happens in S phase?
DNA replication/synthesis, centrosomes replicate
What is a centrosome?
Main microtubule organizers. Pull microtubules and create spindles
What happens in G2 phase?
Preparing for division of cell by checking DNA, making sure there are enough organelle to move on
What is G0 phase?
Inactive
What happens in prophase?
Chromosome condensation
Centrosomes move toward poles
Part of condensation involves binding proteins called…
cohesions
What happens in prometa and metaphase?
Chromosomes captrued by poles
Chromatids line up on metaphase plate
Each sister chromatid is attached to a pole and under tension
Dynein is…
Motor protein that moves toward negative pole of microtubules
What happens in anaphase?
Cohesions holding chromatids and chromosomes separate
What happens in telophase?
Chromosomes decondense
Nuclear envelope begins to reassemble
Microtubules become less dynamic
What happens at the same time as telophase?
Cytokinesis
Contractile ring is an…
actin/myosin cytoskeletal machine
What is the end of goal of mitosis?
Create an identical pair of cells (conservative replication)
What is the end goal of meiosis?
Create cells with half genetic content
Genetic variation
Reductive and recombinatorial
What kind of cells are used in meiosis?
Somatic cells (non sex cells)
End product of meiosis is…
gametes (haploid)
An allele is…
a version of a gene
What happens in prophase 1 of meiosis?
DNA condenses
Homologus chromosomes begin to associate
In late prophase 1 of meiosis, homologus chromosomes begin to align and form…
Chiasmata (cross-over sites)
Why is chiasmata important?
Provides genetic variation
Links homologus pair
What happens in metaphase 1 of meiosis?
Nuclear envelope dissolves and microtubules hunt and find kinetichores
Kinetichores pointing the same way are called…
Syntelic
In metaphase 1 of meiosis, kinetichores for sister chromatids attach..
to the same centrosome/pole
Kinetichore pointing different ways are called
amphisyntelic
Sister chromatids have … attachment
syntelic
homologus pairs have … attachment
amphisyntelic
What happens in anaphase 1 and telophase 1 of meiosis?
Similar to mitosis but
What connects to the centromeres?
Spindle, kinetichores, and hold the sister chromatids together
Microtubules grow from…
centrosomes placed at opposite poles of the cell
Microtubules attach at…
each chromosomes kinetichores
Where does photosynthesis occur in green plants?
chloroplast
The jelly-like structure in chloroplast is called…
stroma
inside the stroma, there are…
grana
one part of the grana is called…
thylakoid
the thylakoid is made of…
thyloakoid membrane and lumen(innermost)
What is the chlorophyll found in PS II of green plants?
P680
What proteins are found in the thylakoid membrane?
PSII, Cytochrome B6F, PSI, ATP synthase
What is the pigment in PSI?
P700
What is the Z scheme?
Combination of the cyclic and non cyclic processes that plants do
How do green plants put an electron back onto P680?
Oxidize water
What are the products of non cyclic green plant photosynthesis?
NADPH and O2 (Byproduct)
Which photosystem is used in cyclic green plant photosynthesis?
PSI
What is the point of cyclic photosynthesis?
Generate a proton gradient to make ATP
What is special about cyctochrome B6F
It can pump protons
What do we call the motion of protons across ATP synthase
Chemiosmosis
Cytochrome B6F pumps protons from the … to the …
stroma; lumen
What are the products of green plant cyclic photosynthesis?
ATP
What is the most abundant enzyme on Earth?
Rubisco
What enzyme catalyzes the first step of the Calvin Cycle?
Rubisco
Photosystems are made of…
pigments
Early Earth was very high in ___ and ___ levels
H2; CO2
What part of amino acids define tertiary structure?
R groups and primary structure
What determines how proteins fold?
Primary structure
Allosteric sites can…
Activate or deactivate enzymes
Active sites can…
Bind substrates
Adjust substrates
Decrease transition energy level
What are cofactors/coenzymes?
Attached to enzyme and required for its activity
Not used in reaction
What are the products of the TCA cycle?
NADH, FADH2
NADH and FADH2 from TCA cycle go to…
ETC
What does the ETC make?
Proton gradient
What is feedback inhibition?
When there is an excess product, so the product binds to the enzyme to inhibit it
What does an allosteric activator do?
Binds to the enzyme to change its shape so that the substrate can bind to the enzyme
What does an allosteric inhibitor do?
Binds to enzyme to change its shape so the substrate cannot bind to the enzyme
Where does glycolysis, fermentation, TCA cycle, ETC, and pyruvate oxidation occur in prokaryotes?
Cytoplasm, Cytoplasm, Cytoplasm, Inner plasma membrane, Cytoplasm
Where does glycolysis, fermentation, TCA cycle, ETC, and pyruvate oxidation occur in eukaryotes?
Cytoplasm, Cytoplasm, mitochondrial matrix, inner mitochondrial membrane, Mitochondrial matrix
How did mitochondria arise?
Archaean engulfed bacterium. Kept it alive instead of digesting and the two formed a symbiotic relationship
How did chloroplast arise?
Eukaryotic cell engulfed a cyanobacteria
What kind of cells have mitochondria?
Animal cells, plant cells, protists, fungi
What kind of cells have chloroplast?
Plant cells
Where are integral membrane proteins located?
Along the lipid bilayer
Where are the peripheral membrane proteins located?
On the inside or outside part of the membrane
How are transport proteins similar to enzymes?
Can be under allosteric control
Can recognize specific substrates
Can couple unfavorable reactions with favorable ones
What are the two kinds of transport?
Active and Passive
What are the two kinds of passive transport?
Simple diffusion, and facilitated diffusion
How is simple diffusion and facilitated diffusion different?
Simple diffusion uses no protein and is not regulated. Facilitated diffusion uses a transmembrane protein and is regulated
What pass through transmembrane proteins?
Polar molecules and ions
What is the difference between passive and active transport?
Passive goes with the concentration gradient (high to low) and active goes against it (low to high)
What are the three proteins found in membrane transport?
Uniport, simport, antiport
What does a uniport do?
Transport one thing in one direction
What does a simport do?
Transport two things in one direction
What does a antiport do?
Transports two things in two directions
What are the two main differences between prokaryotic and eukaryotic cells?
Eukaryotic cells are large and have membrane bound organelles
What is the zip code, road, and vehicle for intracellular transport?
Signal sequences, cytoskeleton, and motor proteins
What are the two types of cytoskeleton for intracellular transport?
Microtubules and actin
What motor proteins use microtubules?
dynein and kinesin
What motor proteins use actin?
myosin
What are the 4 main functions of the cytoskeleton?
Road for motor proteins
Maintain cell shape
Cellular motion
Providing mechanical strength
Cytoskeleton is constantly being…
Formed and deformed
What are intermediate filaments used for?
Mechanical strength, stress resistance, organelle anchoring
What are microtubules used for?
Intracellular transport and cell division
What is actin used for?
Intracellular transport, cellular motion, and maintaining cell shape
What are the three filaments?
Intermediate filaments, microtubules, and actin
What are microtubules made of?
alpha-beta-tubulin dimers
What do the + end and - end of microtubules refer to?
Asymmetry
What can alpha tubulin do?
Bind to GTP
What can beta tubulin do?
Bind to GDP and GTP and hydrolyze GTP
Which end does kinesin and dynein walk towards?
Kinesin: + end
Dynein: - end
What does actin bind to?
ATP
What are the phases of the cell cycle? (Broad)
Grow phase, DNA replication, DNA segregation, cell division
What are the two broad phases a cell spends in?
M phase and interphase
What are centrosomes made of?
Microtubules
What holds sister chromatids together?
Cohesin proteins
What is different about metaphase I?
The centrosomes attach to both sister chromatids to ensure they are separating the homologous chromosomes and not the sister chromatids
What are the four different things about meiosis I?
Recombination by crossing over
Synaptonemal complex
Kinetochore fusion
Centromere cohesin
What are homologous chromosomes held together by?
Synaptonemal complex
bivalent permits…
in chromosomes
recombination
What is the place where homologous chromosomes interact with one another called?
chiasma
If maternal and paternal have same alleles, it is…
homozygous
Dominant:
One copy of allele is needed to obtain trait
Recessive
Two copies of allele is needed to obtain trait
Phenotype
Observable characteristics
What did Mendel do
Observed pea plants
Homologues are independently…
assorted
Chromosomes are made of…
DNA and protein(histones)
Heritable change in a living organism using material extracted from a dead one is called…
transformation
Ex: Making nonvirulent bacteria virulent
What is Mendel’s 1st law of inheritance?
Segregation: Genes are transferred as separate and distinct units from one generation to the next
Answer: dies or lives
Mouse injected with virulent S bacteria
Mouse injected with nonvirulent R bacteria
Mouse injected with virulent S bacteria that was heat treated
Mouse injected with virulent S bacteria that was heat treated and nonvirulent R bacteria
dies
lives
lives
dies
What is Mendel’s 2nd law of inheritance?
Independent assortment: Alleles of a gene in 1 chromosome pair are inherited independently of alleles of a gene on another pair; gametes fuse at random
What is Mendel’s 3rd law of inheritance?
Dominance: One allele possesses a greater influence over the other
What do viruses consist of?
Protein and nucleic acid (DNA or RNA)
Where are viruses injected into?
Injected into the cytoplasm of cells. Substance of virus can be determined based on radioactive material present, particularly, Phosphorus (DNA) and Sulfur (Proteins)
P680 can (reduce/oxidize) …?
Oxidize water
P700 can (reduce/oxidize) …?
Reduce NADP
Lysozyme…
Breaks down cell walls
What factors contribute to denaturing proteins?
Heat, salt, pH
Model for structure of DNA gave an explanation for…
Sequence and code
Replication
Repair
Recombination
DNA strands are (parallel/antiparallel)
Antiparallel
DNA strands have polarity of…
(5’, 3’)
Polymer of DNA
deoxyribonucleic acid
Monomer of DNA
Deoxy-ribose nucleotides (A,C,T,G)
Within strands nucleotides are linked by…
Between strands the bonds are…
Phospho-diester bonds
Hydrogen bonds between complementary bases
The 5’ sugar is linked to…
Phosphate group
Incoming nucleotides are added to the …
3’ end
DNA sequences are always written as …
5’ to 3’
Nucleoside consists of:
Base + sugar (adenosine, guanine, thymine, cytidine)
Nucleotide consists of:
Base + sugar + phosphate
What are the nitrogenous bases
adenine, guanine, thymine, cytosine
DNA backbone is:
sugar - Phosphate - sugar
What are the deoxy-ribose carbons 1’,3’,5’ linked to, respectively?
Base, OH, phosphate
A always bonds with… (__ H bonds)
T (2 bonds)
G always bonds with … (___ H bonds)
C (3 bonds)
Complementarity makes strands…
redundant
DNA replication requires a lot of…
enzymes and factors
In DNA replication, enzymes come together in…
a replication fork that moves in one direction
DNA primase:
Adds RNA primers without initial 3’ OH
What are the three membrane functions mediated by proteins?
Platform for biological reactions
Sensing
Cell recognition
Net direction of a reaction depends on…
Relative potential energies of reactants and products
Potential energies of molecules are determined by…
Structure and concentration
Hydrolysis of ATP could drive…
Formation of concentration gradient
Endergonic reactions
Cytoskeletal reorganization
Intrcellular transport
Muscle contraction
When a photon interacts with a molecule, it can be…
- Scattered - Photon changes direction but no wavelength
- Transmitted - Photon keepd going
- Absorbed - Molecule acquires energy of photon and goes to excited state
When a pigment molecule absorbs a photon, the enrgy can be…
Released as a lower energy photon (fluorescence)
Transferred directly to another nearby pigment (resonance)
Used for a chemical reaction (redox)