BIS 103: The Electron Transport Chain Overview Flashcards
What takes place in the Matrix?
PDH complex
Krebs
B oxidation
amino acid ox
What proteins are in the inner membrane?
complexes 1-IV : respiratory e- carriers
ADP/ATP translocase
ATP synthase
what are mito infoldings called?
cristae
What is the MW limit of mito pores?
5000 MW
what portion of protein are made from mito DNA?
20%
What enables mixin contents in mitochondria?
fusion and fission
Rieske Iron-Sulfur center
2Fe-2Se, with 1 Fe bound to 2 cys, other Fe bound to 2 his
Draw the different iron sulfur complexes
: ]
Redox potential of Fe-S complexes
not absolute - dependent on protein to which is bound
Ubiquinone ring structure
10 isoprene units - very hphobic to stay in membrane
aromatic ring - stabilizes structure, reactive o’s
Ubiquinone rxns - names
add e- and h+
- > semiquinone radical - 1O*, 1 OH
- > ubiqinon - 2 oh’s
goal of ubiqinone
holds h+ and e-, in 1 or 2 pairs at a time
importance of heme rings to scientists
indicates e- passage by changing spectra - something the backbones and R groups can’t do
Heme A differs from others because:
has hphobic tail to embed in membranes
Route of FADH2 e-
complex 2 -> Q ->complex 3 -> cytochrome c -> complex 4
Route of NADH e-
compelx 1: Q ->complex 3 -> cytochrome c -> complex 4
route of H+ per NADH - amounts, totals
complex I - 4
complex 3 - 4
complex 4 - 2
10 total
route of H+ per FADH2 - amounts, totals
complex 3 - 4
complex 4 - 2
6 total
mobile carriers
coQ, cytochrome c
Where does cytochrome C work?
intermembrane space
which side of the mitochondria has a + chaarge?
intermembrane space
where is the N side?
the matrix
average resp e- carriers, differences
10^4 - hearts have more
Complex 2 other names
succinate coQ oxidoreductase
succinate dehydrogenase
