BIS 103: Krebs

Question 1

I - Reactants

Answer 1

oaa, acetyl CoA, H20

Question 2

II - Reactants

Answer 2

L- citrate,

Question 3

III - Reactants

Answer 3

isocitrate, NAD+, Mg+

Question 4

IV - Reactants

Answer 4

a-kg, NAD+, CoA-SH

Question 5

V - Reactants

Answer 5

succinyl-CoA, GDP, Pi

Question 6

VI - Reactants

Answer 6

succinate, FAD

Question 7

VII - Reactants

Answer 7

trans-fumarate, h20

Question 8

VIII - Reactants

Answer 8

L-malate, NAD+

Question 9

I - Products

Answer 9

L-citrate, CoA

Question 10

II - Products

Answer 10

isocitrate

Question 11

III - Products

Answer 11

a-kg, NADH, CO2

Question 12

IV - Products

Answer 12

succinyl-CoA, CO2, NADH

Question 13

V - Products

Answer 13

succinate, GTP, CoASH

Question 14

VI - Products

Answer 14

trans-fumarate, FADH2

Question 15

VII - Products

Answer 15

L-malate

Question 16

VII - - Products

Answer 16

oaa, NADH

Question 17

I - Enzymes

Answer 17

citrate synTHAse

Question 18

II - Enzymes

Answer 18

aconitase - 4Fe:4S

Question 19

III - Enzymes

Answer 19

isocitrate dehyrongenase

Question 20

IV - Enzymes

Answer 20

a-kg dehydrogenase complex

Question 21

V- - Enzymes

Answer 21

succinyl-CoA synthase

Question 22

VI - - Enzymes

Answer 22

succinate dehygrogenase

Question 23

VII - Enzymes

Answer 23

fumarase

Question 24

VIII - Enzymes

Answer 24

malate dehydrogenase

Question 25

I - Mechanism

Answer 25

0. oaa binding induces site for CoA so h20 doesnt attack first 1. CH2-acCoA attacks C=O -> CO2 2. h20 attacks C=O -> CoA-SH

Question 26

II - goal

Answer 26

so you can make a ketone

Question 27

II Mecanism

Answer 27

0. 3 point attachment 1. Fe takes e- density of -OH - more positive 2. enables his H- attack - > db 3. add water to db

Question 28

III - Mechanism

Answer 28

0. MG2+ takes electron density from bottom 1. NAD+ takes bottom H -> db, CO2 leaves 3. keto-enol taut

Question 29

III - significance of mech

Answer 29

co2 leaves, keto-enol taut -> very 1D

Question 30

IV - mech

Answer 30

E1- TPP attack at keto C=O - CO2 leaves E2 - CoAsh attacks C=O E3- FAD->NADH

Question 31

V- mech

Answer 31

P)-O- attacks O=C-S-CoA his: attacks O=C-P -> CoA-SH, suc GDP attacks his: -P

Question 32

V - mech significance

Answer 32

reversible - = E

Question 33

what interchanges GTP and ATP?

Answer 33

nucleoside diphosphate kinase

Question 34

ATP/NADH - how much ATP from krebs?

Answer 34

2.5 ->7.5

Question 35

ATP/FADH2 - how much ATP from krebs?

Answer 35

1.5 -> 1.5

Question 36

how much ATP from krebs (overall)?

Answer 36

10

Question 37

oxaloacetate

Answer 37

O=C --CO2- | CH2-CO2-

Question 38

Citrate

Answer 38

CH2-CO2- HOC --CO2- CH2-CO2-

Question 39

Isocitrate

Answer 39

CH2-CO2- H C --CO2- HO CH-CO2-

Question 40

A-KETOGLUTARATE

Answer 40

CH2-CO2- H C H O=C -CO2-

Question 41

SUCCINYL-CoA

Answer 41

CH2-CO2- H C H O=C -SCoA

Question 42

SUCCINATE

Answer 42

CH2-CO2- H C H O=C -O-

Question 43

FUMARATE

Answer 43

CH-CO2- || H C O=C -O-

Question 44

MALATE

Answer 44

CH-CO2- | HOC O=C -O-