BIS 103: Krebs Flashcards
I - Reactants
oaa, acetyl CoA, H20
II - Reactants
L- citrate,
III - Reactants
isocitrate, NAD+, Mg+
IV - Reactants
a-kg, NAD+, CoA-SH
V - Reactants
succinyl-CoA, GDP, Pi
VI - Reactants
succinate, FAD
VII - Reactants
trans-fumarate, h20
VIII - Reactants
L-malate, NAD+
I - Products
L-citrate, CoA
II - Products
isocitrate
III - Products
a-kg, NADH, CO2
IV - Products
succinyl-CoA, CO2, NADH
V - Products
succinate, GTP, CoASH
VI - Products
trans-fumarate, FADH2
VII - Products
L-malate
VII - - Products
oaa, NADH
I - Enzymes
citrate synTHAse
II - Enzymes
aconitase - 4Fe:4S
III - Enzymes
isocitrate dehyrongenase
IV - Enzymes
a-kg dehydrogenase complex
V- - Enzymes
succinyl-CoA synthase
VI - - Enzymes
succinate dehygrogenase
VII - Enzymes
fumarase
VIII - Enzymes
malate dehydrogenase
I - Mechanism
- oaa binding induces site for CoA so h20 doesnt attack first
- CH2-acCoA attacks C=O -> CO2
- h20 attacks C=O -> CoA-SH
II - goal
so you can make a ketone
II Mecanism
- 3 point attachment
- Fe takes e- density of -OH - more positive
- enables his H- attack - > db
- add water to db
III - Mechanism
- MG2+ takes electron density from bottom
- NAD+ takes bottom H -> db, CO2 leaves
- keto-enol taut
III - significance of mech
co2 leaves, keto-enol taut -> very 1D
IV - mech
E1- TPP attack at keto C=O - CO2 leaves
E2 - CoAsh attacks C=O
E3- FAD->NADH
V- mech
P)-O- attacks O=C-S-CoA
his: attacks O=C-P -> CoA-SH, suc
GDP attacks his: -P
V - mech significance
reversible - = E
what interchanges GTP and ATP?
nucleoside diphosphate kinase
ATP/NADH - how much ATP from krebs?
2.5 ->7.5
ATP/FADH2 - how much ATP from krebs?
1.5 -> 1.5
how much ATP from krebs (overall)?
10
oxaloacetate
O=C –CO2-
CH2-CO2-
Citrate
CH2-CO2-
HOC –CO2-
CH2-CO2-
Isocitrate
CH2-CO2-
H C –CO2-
HO CH-CO2-
A-KETOGLUTARATE
CH2-CO2-
H C H
O=C -CO2-
SUCCINYL-CoA
CH2-CO2-
H C H
O=C -SCoA
SUCCINATE
CH2-CO2-
H C H
O=C -O-
FUMARATE
CH-CO2-
||
H C
O=C -O-
MALATE
CH-CO2-
|
HOC
O=C -O-
