BIS 103: Coupled Enzyme Assays Flashcards
1
Q
What pH do you use?
A
optimum of enzyme in question - others can be in excess - (first enzyme, not the one that you can detect nad with )
2
Q
what is an IU?
A
1 micromol S-P / min
3
Q
A nucleic acid
A
260 (despite #p’s)
4
Q
A protein
A
28-
5
Q
A NAD’s H
A
340
6
Q
acetate kinase
A
acetate - acetyl phosphate
7
Q
Abs vs. time slope
A
linear - Vo
nonlinear - trash
8
Q
how to check for a valid assay
A
if decrease [enxyme in question] by .5, slope will be half as steep
