BIOTECHOLOGY 2

Question 1

gene cloning?

Answer 1

making multiple copies of same gene

Question 2

steps in gene cloning

Answer 2

1 making of rdna
2transformation
3 selection of recombinant clones

Question 3

components of a cloning vector

Answer 3

1 ori
2antibiotic resistance genes
amp R tet R
3 lac Z
4 restriction sites

Question 4

blue white screening

Answer 4

ecoli Lac Z gene to permit visual detection of colonies of plasmids with dna inserts

Question 5

commercially available cloning vectors

Answer 5

pUC—-18,19

pBR —-pBR322,328

Question 6

BACTERIOPHAGE examples

Answer 6

lambda

M13 bacteriophage vectors

Question 7

expression vectors

Answer 7

plasmids engineered to contain regulatory sequence for transcription and translation

Question 8

expression vectors examples

Answer 8

• **strong promoters
• **transciption terminators
• **ribosome binding site

Question 9

promoter?

Answer 9

initiates transcription

***positioned 10-100nucloetides upstream ribosome binding site

Question 10

ribosome binding site is also called

Answer 10

shine dalgarno sequence

Question 11

what are histidine tags

Answer 11

N OR C terminal fusion of heterologous proteins to short peptidee(tags)

Question 12

examples of expression vector

Answer 12

prokaryotic
eukaryotic expression system—-bacterial expression system

yeast.viral,mammalian cell expression vector