biotechnology principle and process Flashcards
define biotech
the techniques of using living organims for products and services
example of modern biotech
in vitro fertilisation, vaccines, therapeutic drugs
acc toeuropean federation of biotech defination of biotech
the integration of natural science and organisms, cells, parts there of and molecular analogues for products and services
organisms whose gene has been manipulated of modified
genetically modified organisms
steps of genetic engineering
construction of rdna
gene transfer
gene cloning
explain biochemical engineering
providing a sterile environment that is contamination free to only enable the growth of desirable gene
when and who constructed 1st recombinant DNA
1972 by stanley cohen and herbert boyer
what are restriction enzyme
these enzymes belong to a category of enzymes called nucleases
total no of restriction enzyme obtained from approx 230 strains of bacteria
900
which was 1st restriction enzyme
hind 2
seperation technique for DNA known as
gel electrophoresis
steps of PCR
denaturation
annealing
extension
vehicles which carry gene of interest known as
vectors