biosafety Flashcards
primary and secondary biosafety
primary biosafety is protection of personall and immediate laboratory environment. via good microbiological practices and adequate safety equipment
secondary biosafety is protection of the externa environment via facility design and operational processes
4 primary controls of biosafety
- PPE
- administrative controls
-engineering controls (facility design) - standard operating procedure
route of exposure to biological hazards
inoculation, inhalation, contamination of skin and mucous membranes and ingestion
why are facilty designs put in place
- provide a barrier to protect staff
- protect the community
- prevent aerosol transmission
facility designs to prevent aerosol transmission
- air locks
- air treatment systems
- ventilation systems
- controlled access zones
- seperate building or modules for isolation
factors used to determine risk groups
pathogenicity
infectiours dose
mod eof transmission
avaliability of treatment and preventative measures
host range
what are biosaftey levels and what do biosafety system include?
provides the user with a descripition of the minimum biosafety required to handle the organism safelky
engineering
operational
technical and physical requiremnts
biosafety levl 2 for risk group 2
good lab practices, PPE, biological saftye cabinet
generating aerosols
- inoculating- loop manipulation
Sub culturing and streaking culture media
‘Cooling’ a loop in culture media
Flaming a loop
-pipettes
Mixing microbial suspensions
Pipette spills on hard surfaces
-needle and syringe mainuplation
Expelling air
Withdrawing needle from stopper
Injecting animals
Spray created when needle separates from
syringe
-others
Centrifugation
Using blenders, shakers, sonicators, and
mixing instruments
Pouring or decanting fluids
Opening culture containers
Spillage of infectious material
biosafety level 1, containment level 1 for risk group 1
basic level of biosafety
good microbiological practices and procedures
open bench owrk
-controlled access, hand washing sink, PPE, sharp hazard warning sign, lab bench and rarely autoclave
biosafety level 2 ,containment level 2 for risk group 2
use of primary barriers: good microbiological practices and PPE
-controlled access, hand washing sink. PPE, sharp hazard warning sign, lab bench, autoclave and BIOLOGICAL SAFETY CABINET
biological safety level 3 containment level 3
emphasis on primary and secondary barriers. -good microbiological practices
- PPE and powered air purifying respirator
- biological safety cabinet
-self closing double door access, controlled access
- directional airflow
- autoclave for waste treatment and other uses
- effluent decontamination system
- observation window
- exhaust air to be HEPA filtered
- 100% outside air is supplied
- anteroom
personal shower out
- sealed penetrations
biological saftey level 4
-max biosafety levels
- airlock entry
- self closing double door acces and electronicaly controlled second door
- class III biological safety cabinet
- double ended autoclave
- PPE positive pressure suit and powered air purifying respiator
- anteroom
- 100% outside air supplied
- supply and exhaust air is HEPA filtered
- chemical shower out
- personal shower out
- effluent decontamination system
- waste is autcolaved (special waste disposal)
risk assesment to give appropriate biosafety leevl
- potential for aerosol generation
- type quantity and concentration of infectious agents produced
- agent stability in the work env
- type of work proposed (in vivo, in vitro and aerosol challenge studies)
- use of recombinant organisms
what is biosecurity for
to prevent theft, misuse or intentional release of pathogens for the
- advancement of science
- diagnosis of agents causing disease or
- misuse of technologies
