Biomolecules Flashcards
What is the biological test for starch?
- Add a few drops of iodine solution
2. Record any colour change
What is the colour change for the biological starch test?
Yellow to Dark Blue/ Black (for a positive result)
What is the biological test for reducing sugars?
- Add 2cm’ of Benedict’s reagent to the solution
- Place in a 90° - 100° water bath for 5 mins.
- Record any colour change
What is the colour change for the biological test for reducing sugar?
Blue to Red (for a positive result)
What is the biological test for non-reducing sugars?
- Add 1cm’ of 1M of hydrochloric acid to solution
- Place in a 90°-100° water bath for 2 mins
- Neutralise with sodium hydrogen carbonate
- Add 2cm’ of Benedict’s reagent
- Place in a 90°- 100° water bath for 5 mins
- Record any colour change
What is the colour change for the biological test for non-reducing sugar?
Blue to Red (for a positive result)
What is the biological test for a protein?
- Add 2cm’ of 0.4M sodium hydroxide to solution
- Add 0.4M copper (II) sulphate drop by drop
- Shake gently and record results
What is the colour change for the biological for a protein?
Blue to Purple (for a positive result)
What is the biological test for a lipid?
- Shake solution with absolute ethanol for 1 min
- Add 2cm’ of water and shake well
- Record observations
What is the colour change for the biological for a lipid?
Clear to a milky solution (for a positive result)
What are the three types of carbohydrates?
- Monosaccharides
- Disaccharides
- Polysaccharides
Which two carbohydrates for crystals, are soluble, and ?
Monosaccharides and Disaccharides
What are the 4 functions of carbohydrates? (In humans and plants)
- Instant energy source
- Transportable form of energy
- Stored as glucose
- Structural material ( in plants)
What is the formula for Glucose?
C6H12O6
What is the definition of a disaccharide?
Two monomers joined together by a condensation reaction
What is the definition of a condensation reaction?
A reaction between two hydroxyl groups where water is lost and a glycosidic bond is formed.
What type of bond is C-O-C?
A glycosidic bond
What is made from Glucose + Glucose?
Maltose
What is made from Glucose + Galactose?
Lactose
What is made from Glucose + Fructose?
Sucrose
What is the definition of a hydrolysis reaction?
Splitting a glycosidic bond using a molecule of water
What is the general formula of a monosaccharide?
C(H2O)n
What is a ring with 6 carbon atoms called?
A Hexose
Why is glucose a suitable sugar? (4 reasons)
- Its very soluble so its easily transported in plasma
- A polar molecule (uneven charge within the molecule)
- Small so easily crosses membranes
- Major respiratory substrate is readily broken down in respiration and energy release to make ATP
Why is chitin good for animal/insects’ exoskeleten?
- Its waterproof which prevents water loss
- Its strong for support
- Its light weight for flight
What is the structure of Glycogen? (3 points)
.Alpha glucose
.More Branched
.1-4 and 1-6 glycosidic bonds
What is the function of starch?
Storage in plants
What are the two types of starch?
Amylose and Amylopectin
What is the structure of amylose?
Straight chain
What is the structure of amylopectin?
Curved/ Coiled chain
What is the structure of starch?
.Alpha molecules joined in a straight chain with similar bonds
. 1-4 glycosidic bonds
. Either amylose or amylopectin
Why does amylopectin form a coil?
Amylopectin forms a coil structure for a more stable structure of molecules, iodine molecules slot between the coil to cause the colour change in the iodine test.
What is the function of cellulose?
Used for structure in plants
What bonds join the linear chains in cellulose?
Hydrogen bonds
In cellulose 60-70 chains make what?
Microfibril
In cellulose several microfibrils make what?
A macrofibre
What is the structure of cellulose?
. Beta glucose
. B-Glycosidic bonds
. Every other B-Glucose is flipped 180°
Chitin is a polysaccharide made of what?
Beta Glucose
Why do polysaccharides store glucose in its insoluble form?
To prevent an osmotic effect
What is the structure of chitin?
Same structure as cellulose but has nitrogen, creating a Acetyl amide side groups.
What elements do lipids contain?
Carbon, Hydrogen, and oxygen. (Phosphate lipids also contain phosphate)
Are lipids polar or non-polar ? Do they dissolve?
Non-polar so do not dissolve (but do dissolve in non-polar substances like organic solvents)
What are the 5 functions of lipids?
- Energy source and storage: 1g of lipid= X2 of carbs
- Protection: protecting organs from physical damage
- Thermal insulation
- Oxidation: of triglycerides to produce metabolic water
- Phospholipids: are a component of cell membranes and electrical insulation of nerves.
- Waxes and Oils: waterproofing
What are three types lipid chains?
- Unsaturated
- Saturated
- Polyunsaturated
What is the definition for a saturated chain?
A chain where all the carbon atoms are joined by single hydrogen bonds.
What is the definition for a unsaturated chain?
A chain with carbon to carbon bonds
What is the structure of a saturated chain?
Straight chain
What is the melting point of a saturated chain?
High melting point
What is the structure of a unsaturated chain?
Bents chain
What is the melting point of a unsaturated chain?
Lower melting point
What is the definition of a polyunsaturated chain?
A chain with more than one carbon to carbon bond
Are triglycerides polymers?
No, they are three fatty acids and a glycerol
What bond is found in a triglyceride?
Ester bond
What is the definition of a fatty acid?
A long molecule with a polar hydrophilic end
How are triglycerides and phospholipids created?
During a condensation reaction
How are triglycerides and phospholipids broken down?
During a hydrolysis reaction
What do phospholipids have instead of a third fatty acid?
A phosphoric group
What do phosphoric groups create in a phospholipid?
A hydrophilic head and hydrophobic tail
What is the biomolecule test for lipids?
- Add ethanol which dissolves any lipids present
- Add water
- A positive result forms a milky solution
What do fatty deposits cause?
Obstructions in the blood vessels
What does high blood pressure cause?
Hypertension
What 4 factors contribute to heart disease?
- Diet high in saturated fats
- Smoking
- Lack of exercise
- Alcohol
What do low density lipoproteins cause?
Atheroma to form which blocks blood vessels
How do high density lipoproteins effect the body?
These are good for the body
What elements are found in proteins ?
Carbon, Hydrogen, Oxygen, Nitrogen, some contain sulphur and others contain phosphorus.
What is the monomer that mades up a polypeptide chain?
Amino Acid
What is the polymer called that consists of amino acids?
Polypeptide chain
What is the N-terminal and where is it found on an amino acid?
- NH2, at one end of the molecule called the N- terminal
What is the -COOH group on a amino acid called and where is it found?
A Carboxyl group, at the other end of the molecule called the C-terminal
Which element of an amino acid is different in each one?
The R-Group
What is the bond found between amino acids in a polypeptide chain?
Peptide Bond
What reaction takes place during the formation of a polypeptide chain?
Condensation Reaction
What reaction takes place during the breaking down of a polypeptide chain?
Hydrolysis Reaction
What is the structure of a primary protein structure?
A linear chain of amino acids
What bond is made between adjacent amino acids in the primary structure?
Covalent bonds
What bonds are between amino acids in a primary structure protein? How are they formed?
Peptide bonds made in a condensation reaction
When does hydrolysis occur in the human body?
During digestion
What is the structure of a secondary protein?
A polypeptide chain which may take on a regular arrangement. Ones with small R-groups will form a Alpha helix and those with large R-groups create and Beta pleated sheet.
What bonds hold the structure of secondary proteins together?
Hydrogen bonds
What secondary structure do proteins with small R-groups form?
Alpha Helix
What secondary structure do proteins with large R-groups form?
Beta Pleated Sheet
Can the shape of a tertiary structure protein change?
Yes, globular proteins are susceptible to change
What is the structure of a tertiary protein?
The peptide structure folds in a 3D shape, creating a globular protein
What bonds are present in a tertiary structure protein?
Ionic bonds, Hydrogen bonds, Disulphide bonds, and Hydrophobic bonds
What is the structure of a quaternary protein?
Several tertiary-structure peptide chains fitted together
What bonds hold the quaternary structure of a protein together?
Weak interactions between R-groups
What is the special feature of a quaternary protein?
It can carry a prosthetic group eg. haem in haemaglobin
What is haemoglobin?
A globular protein involved in metabolism
When is oxyhemoglobin formed?
It is created when it bond with oxygen
What is the structure of haemoglobin?
FOUR folded polypeptide chains and FOUR iron containing haem groups
What is the shape of haemoglobin?
Compact and spherical
Is haemoglobin soluble?
Yes
How are haemoglobin proteins arranged?
Hydrophobic ends point in, Hydrophilic ends point out
What is meant by the term conjugated protein?
A protein and non-protein prosthetic group
What is the function of haemoglobin?
To carry oxygen from the lungs to tissue and CO2 from the tissue to the lungs in the blood
What are two properties of haemoglobin?
3D shape
Soluble in water
What is the structure of collagen?
Three identical left handed helix peptide chains wound around each other to form a triple helix
What is the function of glycine in collagen?
Its small so allows the chains to pack closely together
What is every third amino acid in each peptide chain in collagen?
Glycine
What bonds hold the three strands in collagen together?
Hydrogen bonds
What gives collagen its strength?
Collagen molecules cross-link through covalent bonds to form fibres.
What is the function of collagen? (2)
- Gives strength to bones, muscles, skin, and tendons
2. Provides strength and structure
What are the two properties of collagen?
- Insoluble in water
2. Strong and tough
Why is it important in animals that water works as a universal solvent?
Allows molecules such as glucose to dissolve, to be transported around the body. Its polar charge means it can dissolve molecules which allows for oxygen to be transported to fish in water.
What is meant by ‘universal solvent’?
Both polar and non-polar molecules can dissolve in it
Why is it important in plants that water works as a universal solvent?
Allows gases like oxygen and CO2 to diffuse in and out of the water being transported around the plant.
Why can gases dissolve in water?
They molecules are attracted to the polarity of the water
Why is it significant to animals that water is a reactant in hydrolysis?
Aids the breakdown of polymers
Why is it significant to plants that water is a reactant in hydrolysis?
Used in the synthesis of ATP during photosynthesis
What is cohesion between water molecules? Why does it occur?
Hydrogen bonds between water molecules means water has high levels of cohesion. This means the water “sticks” together. In large amounts the hydrogen bonds are quite strong and cause a high surface tension.
Why is cohesion of water important for animals?
Provides a stable surface for insects to ‘skate’ across in their environment.
Why is cohesion of water important for plants?
Means streams of cohesion water can be “pulled” up the xylem tissue
What is meant by high latent heat of vaporisation?
When changing state heat energy is taken in or expelled. This is latent heat. This means that water requires large amounts of energy to change state
Why is high latent heat of vaporisation important for animals?
Prevents easy loos of water which would lead to dehydration
Why is high latent heat of vaporisation important for plants?
Provided structure as water is retained in the plant
Why is it important in animals that water has a high specific heat capacity?
Means the temperature of the body wont vastly increase which prevents the denaturing of enzymes
Why is it important in plants that water has a high specific heat capacity?
Ensure water stays in cohesion for transpiration
Why is it significant that water is less dense as ice than water as a liquid?
It allows ice to form an insulating layer on the top of ponds/ lakes when frozen to maintain an environment for biodiversity.
Why is it important that water is transparent?
Allows sunlight to pass through for photosynthesis in aquatic plants.