Biology - Microscopes and Cells Quiz Flashcards
what do microscopes do
magnify objects and make small things appear larger
what is the main benefit of a microscope
resolution
whats resolution
the ability to see fine detail
whats a compound microscope
microscope with 2 or more lenses
what is the most common type of microscope
compound
what do you use a compound microscope for
to look at transparent specimens
what is the max magnification of a compound microscope
1000x
how does a compound microscope produce an image
passes light through the specimen
what’s the structure of a compound microscope
1 eyepiece and 3 objective lenses
what is a dissecting microscope used for
to look at solid objects
whats the max magnification on a dissecting microscope
85x
what’s the structure of a dissecting microscope
2 eyepieces and 2 light sources
what are the advantages of a dissecting microscope
images are produced in colour and 3-d/liign material can be used
what are the disadvantages of a dissecting microscope
low magnification and poor resolution at high magnifications as the light rays are bent
what is transmitted light
light passing through a specimen
whats incident light
light reflecting off a specimen
whats parfocal
concept that when the microscope is focused on one power it should still be in focus when switched to another power
whats an inverted image
when the microscope shows it upside down and backwards
whats a virtual image
when it shows the same as it really is
what are the two meanings of magnification
- the number of times a microscope enlarges the specimen
- the number of times bigger a diagram is when compared to the actual size of a specimen
what is field of view
the diameter of what you see
what happens if something is on low magnification in relation to its field of view
you will see more around the object than if it was on high magnification
what is the fit number
an estimate of the number of times an object can be placed across the diameter
what is scale used for
to compare the diagram size with the actual size of the specimen
what did the use of stains or colouring agents show
that particular stains attached to particular parts of the cell which improved the contrast between internal structures and produced better images
what is the disadvantage of staining techniques
it kills the cells, making it not possible to view living tissue with this technique
whats resolving power
the ability to distinguish between two structures that are very close together
how does an electron microscope work
uses a beam of electrons instead of a light wave and is able to produce images that provide fine detail
how does the transmission electron microscope work
depends on a beam of electrons passed through very thin section of fixed and stained tissue imbedded in plastic
what did the microscope make possible
the obtaining of evidence for or against generally accepted opinions or theories about living things
whats spontaneous generation
the idea that life could emerge spontaneously from non-living matter
what does the occular lense do
magnify specimen
funciton of fine focus
makes tiny adjustments to focus specimen
revolving nose peice function
urns objective lense power
diaphragm fucniton
adjust the amount of light
base function
supports and stabilizes
hinge function
changes the angle
function of stage clips
hold the slide in place
arm function
support of microscope
objective lens function
3 different lenses that magnify the object at different rates