Biology Lab Practical Flashcards

1
Q

Abbrev for Mass

A

Grams (g)

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2
Q

Abbrev for Length/Distance

A

Meter (m)

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3
Q

Abbrev for Liquid volume

A

Liter (L)

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4
Q

Kilo (k)

A

1000 (or 1 x 10^3)

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5
Q

Mili (m)

A

0.001 (or 1 x 10^-3)

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6
Q

Micro

A

0.000001 (or 1 x 10^-6)

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7
Q

Nano (n)

A

0.000000001 (or 1 x 10^-9)

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8
Q

Metric conversion for Mass

A

1 kilogram (kg) = 1000 grams (g)
1 g = 1000 milligrams (mg)
1 mg = 1000 micrograms (μg)

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9
Q

Metric conversion for Distance

A

1 meter (m) = 100 cm
1 centimeter (cm) = 10 millimeters
(mm)
1 mm = 1000 micrometers (μm)
1 μm = 1000 nanometers (nm)

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10
Q

Metric conversion for Volume

A

1liter(L)= 100 centiliters(cL)
1liter(L)= 10 deciliters(dL)
1 liter (L) = 1000 milliliters (mL)
1 mL = 1000 microliters (μL)

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11
Q

Converting a LARGER unit to a SMALLER unit you….

A

MULTIPLY the larger unit by the conversion factor.

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12
Q

Converting a SMALLER unit to a LARGER unit you…

A

DIVIDE the smaller unit by the conversion factor.

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13
Q

Dependent Variable

A

The effect, or response variable
Typically the effect of the Independent variable

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14
Q

Independent Variable

A

The cause or manipulated variable
Typically the what is being tested

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15
Q

Equation to calculate Accuracy

A

% error

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16
Q

Equation to calculate Precision

A

% SD (standard deviation)

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17
Q

Standard Deviation (SD)

A

is a measure of how much variation occurs in a set of measurements (at least three) when compared to the Mean.

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18
Q

The T-Test that = P VALUE
When dealing with a hypothesis

A

the t-test is a P VALUE, which can be thought of as the probability that there is NO difference between the means.
P > 0.05, the null hypothesis is accepted
P ≤ 0.05, the null hypotheses is rejected and the alternative is accepted

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19
Q

Spectroscopy

A

study of the absorption and emission of light and other radiation by matter

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20
Q

Passive transport

A

When molecules move through the membrane and into the cell without using energy ( with transport proteins /without).
- Moves WITH the concentration gradient. (High to Low)

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21
Q

Active transport

A

When a molecule requires energy to preform exocytosis, which moves the molecules against the concentration gradient (Low to High)

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22
Q

How many chromosomes are in a human body?

A

23 pairs

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23
Q

Total magnification

A

= ocular mag. X objective mag.

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24
Q

Microscope parts: Objective lens

A

The rotating dial that has different lens sizes for magnification of the image

25
Microscope parts: Stage
The platform that you place a side. Stage is movable with two knobs located right under it
26
Microscope parts: Light source
Contains a bulb to generate light to pass through the object on the side
27
Microscope parts: Iris diagram
Part of the condenser unit directly below the stage and acts like a camera iris, helps focus’s light on two the object on the side
28
Microscope parts: Condenser
Provides the best balance of resolution and contras Focusses the light on the specimen by allowing it to move up and down
29
Microscope parts: Coarse focus
Helps bring the objective to focus in large steps. Is only used on 4x and 10x objectives!
30
Microscope parts: Fine Focus
Alters the focus in small increments
31
Microscope parts: Slide Clamp
The clamp that holds the slide in place
32
Resolving distance RD
The minimum distance two objects must be apart to be seen as separate
33
Resolution
Is the ability to distinguish two nearby objects as separate
34
Dialysis membrane
Composed of synthetic polymers that interlock
35
Chloride ion stain
sliver nitrate Positive — white precipitate Negative — clear solution
36
Starch stain
Iodine Positive — purple/black color Negative — orange/yellow color
37
Protein (albumin) stain
Biuret reagent Positive — lavender/ violet Negative — light blue
38
Osmosis
The movement of free, unbound water molecules across a membrane.
39
Alternative hypothesis
Is always phrased in case the null hypothesis is not supported by the t-test. If not supported, you may conclude that there is a SIGNIFICANCE between the two means.
40
Null hypothesis
which states that the means of the two groups are the same (i.e. NO difference)
41
Cell wall
The wall surrounding the plasma membrane
42
Acceptable Precision
< or = to 5% SD from the mean
43
R^2
Has to do with the CORRELATION of p-value Range from 0 (0%) to 1 (100%); the higher the R2, the more precisely you can predict the value of the dependent variable based on the value of the independent variable, or the better the correlation between the two variables.
44
G1
1st gap phase - cell enlargement
45
S
Synthesis phase; DNA replication During this stage the chromosomes duplicate
46
G2
2nd gap phase; cells prepare to divide
47
M phase
Mitosis and cytokinesis Cells divide
48
G0
Becomes its own cell/separate from cycle
49
Meiosis
Producing haploid gametes
50
Mitosis
Produces identical daughter cells
51
Prophase
Chromosomes gather together
52
Interphase
Stages G1, S, G2
53
Metaphase
All chromosomes gather in the middle of the cell in a line
54
Anaphase
Chromosomes are pulled to either side and separate
55
Telephase
Cells begins to divide
56
Haploid
Having 1 copy of each chromosomes
57
Diploid
Having 2 copies of each chromosomes
58
P value for best fit line
Is the correlation between the IV and the DV is real or by chance P> 5% or 0.05 = correlation is due to chance P< or = 5% or 0.05 = real correlation
59