Biology AS Chapter 3 - Cell Structure Flashcards
How do you calculate magnification?
Size of image / size of real object
What is resolution?
The minimum distance apart 2 objects can be in order for them to appear separate
What is cell fractionation?
The process where cells are broken up and the different organelles they contain are separated out
Why must the solution the cells are placed in before cell fractionation be cold, buffered and isotonic?
Cold - reduce enzyme activity that might break down organelles
Isotonic - same water potential as the cell so organelles do not shrink or burst by osmosis
Buffered - so the pH does not fluctuate and alter organelle structure or enzymes
What is homogenation?
Cells are broken down by a homogeniser which releases the organelles from the cell. The resultant fluid, known as the homogenate, is then filtered to remove any complete cells or large pieces of debris
What is ultracentrifugation?
The process by which the fragments in the filtered homogenate are separated in a centrifuge
What is the process of ultracentrifugation?
- The tube of filtrate is placed in the centrifuge and spun at a slow speed
- The heaviest organelles, the nuclei, are forced to the bottom of the tube where they form a thin sediment
- The fluid at the top of the tube (supernatent) is removed, leaving the nuclei
- The supernatent is transferred to another tube and spun in the centrifuge at a faster speed than before
- The next heaviest organelle, the mitochondria, are forced to the bottom
- The process is continued such that with each increase in speed, the next heaviest organelle is sedimented and separated
What are the two main advantages of an electron microscope?
- Electron beam has a very short wavelength and the microscope can therefore resolve objects well, it has a high resolving power
- As electrons are negatively charged the beam can be focused using electromagnets
What are the parts of the nucleus and what do they do?
- Nuclear envelope - double membrane which controls the entry and exit of materials
- Nuclear pores - allow the passage of large molecules out of the nucleus
- Nucleoplasm - material that makes up bulk of nucleus
- Chromosomes - consist of protein bound, linear DNA
- Nucleolus - small region which manufactures ribosomal RNA and assembles the ribosomes
What are the functions of the nucleus?
- act as the control centre of the cell through the production of mRNA and tRNA and hence protein synthesis
- retain the genetic material of the cell in the form of DNA and chromosomes
- manufacture rRNA and ribosomes
What are the parts of the mitochondria and what do they do?
- Double membrane - controls what enters and exits the cell
- Cristae - extensions of the inner membrane which provide a large surface area for the attachment of enzymes and proteins
- Matrix - contains proteins, lipids, ribosomes and DNA which allows the mitochondria to produce their own proteins
What is the role of the mitochondria?
The sites of aerobic respiration and are therefore responsible for the production of ATP
What are the parts of the chloroplasts what do they do?
- Chloroplast envelope - double plasma membrane that controls what enters and exits the cell
- Grana - stacks of thylakoids. Where the first stage of photosynthesis occurs
- Stroma - fluid filled matrix where the second stage of photosynthesis occurs
How are chloroplasts adapted to their function of harvesting sunlight and carrying out photosynthesis?
- Granal membranes provide a large SA for attachment of chlorophyll, electron carriers and enzymes
- Stroma possesses all the enzymes needed to make sugars in the second stage of photosynthesis
- Chloroplasts contain DNA and ribosomes so can quickly and easily manufacture some proteins needed in photosynthesis
What is the endoplasmic reticulum?
3D system of sheet like membranes which spreads through the cytoplasm of cells
What is the RER and its functions?
The RER has ribosomes on its outer surfaces of its membranes. Functions:
- provide a large SA for the synthesis of proteins and glycoproteins
- provide a pathway for the transport of materials throughout the cell
What is the SER and its functions?
The SER lacks ribosomes and is more tubular in appearance. Functions:
- synthesise, store and transport lipids and carbohydrates
What is the structure of the golgi apparatus?
Consists of a stack of membranes that make up flattened sacs, or cristernae, with small rounded hollow structures called vesicles.
What are the functions of the golgi apparatus?
- add carbohydrate to proteins to form glycoproteins
- produce secretory enzymes, such as those secreted by the pancreas
- secrete carbohydrates
- transport, modify and store lipids
- form lysosomes
How are lysosomes formed?
When the vesicles produced by the Golgi apparatus contain enzymes, such as lysozymes which hydrolyse the cell walls of certain bacteria