Biology Flashcards
What makes primary structure of proteins?
peptide bonds
What holds secondary structure of proteins?
folded chains (beta sheets and alpha helix) made of hydrogen bonds
What holds tertiary structure of proteins?
3D folded, hydrogen bonds, ionic bonds, dipole, dispersion, and disulfide
What holds quarternary structure of proteins?
multiple peptide subunits held by disulfide bonds
Which amino acids are important in alpha helix?
Proline and glycine- they destabilize alpha helix
Targets of phosphorylation
serine, threonine, tyrosine (Have OH nucleophile)
Which amino acids can mimic phosphate groups
asparatic and glutamic acid (bulky, negative)
What is a salt bridge?
types of bonds?
base and acid interact with hydrogen bonds and electrostatic interactions
Apoprotein vs. haloprotein
apoprotein is without prosthetic group, haloprotein has prosthetic
Isoelectric effect
pH when net charge on protein is zero, high pKa= high pH
Isoelectric focusing
separates protein based on difference in charge. They migrate until pH=pI (until neutral), uses a pH gradient, (-) cathode, and (+) anode
How does SDS work
denatures and gives uniform charge to seperate proteins by mass
How does Native Page work
Separates proteins based on size and charge … retains structure (no denaturing)
How is BME used in SDS page?
Breaks down disulfide bonds, is a reducing agent
Myosin
thick filament, muscle contraction. Uses ATP to crawl along actin
Kinesin
Aids movement of chromosomes and vesicles - intracellular transport
Dynein
movement of cilia and flagella in microtubules - intracellular transport and motility
What types of cells are pluripotent vs. multipotent
embryonic is pleuripotent, adult is multipotent
What crosses membrane?
Small, non-polar molecules
What makes a phospholipid
phosphate head, glycerol backbone, and fatty acid tail
What is hydrostatic pressure
pressure of liquid on container, reflects volume in a space
What is osmotic pressure?
Pressure required to prevent movement across semi-permeable membrane. Shows protein content of blood
Symport
couple energy to move in same direction (one down gradient, other against)
Secondary active transport
indirectly uses energy to move
Intercalated discs
In cardiac muscle, permits passage of ions between cells- allows rapid spread of action potential during contractions
Desosomes
connections between cells that ions/water can flow between. In the skin or intestine
Gap junctions
Tunnels between cells, allows ions/water to flow. Common in cells that use electrical coupling (cardiac, neurons)
Enzyme linked receptor
receptor tyrosine kinase, triggered by signals from the enviornment
Receptor tyrosine kinase
phosphorylates, acts as a dimer that crosslinks when ligand binds. Cross link activates other side. Regulates signal transduction and gene transcription
Smooth ER
lipid production, detoxifies, metabolizes carbs
Rough ER
protein production; exports out of cell and some modification
Golgi Apparatus
sorts, packages, protein modification, exports
Peroxisome
lipid destruction, contains oxidative enzymes, isolate peroxide and break it down
Lysosome
protein destruction, acidic enviornment for enzymes (protects cells- won’t work in cytoplasm)
Eukaryote vs. prokaryote
eukaroyotes compartmentalize, have membrane bound organelles, mitosis, nucleus. Prokaryotes divide by binary fission
Nucleolus
has DNA that makes rRNA. Ribosomes move through pore to cytoplasm
Mitochondira
make ATP, outer membrane with lipid bilayer and inner membrane with cristae… inter membrane is between outer and inner
Reactions and locations of cellular respiration
glycolysis: 6C to 2 3C pyruvate (cytoplasm), PDH complex (pyruavte to acetyl-CoA in matrix), Krebs uses acetyl coA to make NADH, FADH2 in matrix, and ETC which uses electron carrier to make ATP in the inner membrane
Microtubules
mitotic spindle, cilia, and flagella. has 9+2 arrangement
Intermediate filaments
permanent, has many proteins. Aids structural support, and resists stress/ compression
Microfilaments
in cytoplasm, made of actin. Aids movement and cell division. Can lengthen and shorten to move cell
4 parts of cell theory
cell is the basic unit, cells come from pre-existing cells, all living things are composed of cells, cells have DNA
Protists
eukaryote, unicellular, have nucleus and exist in moist enviornment, Not plant/fungus/animal
Archaea
prokaryotes, extreme enviornments (heat, salt, gas) -different cell wall
Bacteria
capsule, cell wall, membrane, flagella, nucleoid, pilli. NO NUCLEUS, circular dsDNA, some have plasmid
gram positive bacteria
purple, has membrane and thick peptidoglycan, capsule
gram negative bacteria
red, has inner membrane, outer membrane, thin peptidoglycan, lipopolysaccharide, capsule- More layers so purple washed off due to thin peptidoglycan and high fatty acid
Bacilli
Rod
Viruses
capsid (protein coat), and either ssDNA or RNA - no organelles, small, need host
bacteriorphage
viruses that infect bacteria, inject phage and use receptors or fuse with membrane
lytic
take over cells machinery to make copies, form functional viruses and then lyse cell to release- FAST but kills host
lysogenic
sneaks in, combine with hosts genetic information. Repressed, then replicates with bacteria. Provirus
retrovirus
enveloped, use reverse trancriptase to converse RNA to DNA to integrate into host genome
viroid
ss Circular RNA, infects plants. Self cleaves to reproduce
prions
infectious proteins, no genetic material- usually beta sheet
Mitosis checkpoints
G1/S and G2/M
G1 of mitosis
makes organelles, proteins, cyclines
G2 of mitosis
makes microtubules
Zona pellucida
outside of egg cell
What derives from endoderm
GI, lungs, liver, pancreas, stomach, SI, LI
What derives from mesoderm
skin, muscles, bone, kidney, bladder, ovaries
What derives from ectoderm
outer skin, sweat glands, hair, NS
stem cells
self renew, can differentiate into specialized cells if embryonic, somatic SC are used for repair
pluripotent stem cells
can differentiate into several types of cells
epithelial stem cells
can only form epithelial cells (utipotent)
hematopoietic stem cells
gives rise to many types of cell but in one family (multipotent)
Asymmetric replication of stem cells
form mother cell (identical) and daughter cell which is differentiated and becomes more specialized
Symmetric replication of stem cells
form two identical cells
induced pluripotent stem cells
revert back into pluripotent and can replace damage
Paracrine signalling
nearby (neurotransmitter, histamine)
Endocrine signalling
signal to large group using blood
Autocrine signalling
signal to site on the same cell
Juxtacrine signalling
signals nearbycells that are in direct contact
Hayflick limit
how many times a cell can divide before senescence (about 60)
Capases role in apoptosis
activates by cytochrome C, break down proteins after aspartate in apoptosis
gray matter
neurons somas, deep in spine, outer of brain
white matter
myelinated axons, deep in brain, outer of spine
cerebellum
coordinates movement, position, motor plan
brain stem
midbrain, pons, medulla, reticular formation - neurons, autonomic, control, cognition, emotion
Corpus callosum
travel between hemispheres
Basal ganglia
motor function, cognition, emotion
Thalamus
sensory, higher brain function
hypothalamus
controls pituatary gland, endocrine, higher functioning, makes ADH and oxytocin
Cerebral Cortex
gyri, sulci, fissures, frontal, parietal, temporal … has sensory, motor, and association areas
Broca vs. Wernicke
Broca is speech production, Wernickes is word meaning
CT scan
X ray to image brain
MRI
radio waves and magnetic field causes atoms to align and signal
EEG
electro activity of brain via electrodes- shows engagement
MEG
electrical currents in the brain
fMRI
functional MRI, shows active structures, firing means more oxygenated
PET scan
CAT + MRI, inject glucose, and shows active areas
Autonomic NS
unconcious, efferent neurons control smooth, cardiac, muscle, and gland cells
Dendrites
how information comes in, then goes to axon as graded potential
Afferent neurons
sensory, periphery to CNS
Efferent neurons
motor or autonomic, away from CNS to periphery
Astrocytes
glial cells of CNS, make scars, homeostasis, BBB, clean synapse
microglia
glial cells of CNS, secrete cytotoxic cells (phagocytosis) and antigen presentation
ependymal cells
glial cells of CNS, barrier between cerebrospinal and interstitial fluid. Helps move fluid
Oligodendrocytes
glial cells of CNS, create myelin sheath and aid exchange between cells
Schwann cells
glial cells of PNS from neural crest, support nerve cells and create myelin- only one axon
Depolarization
excitatory, move towards threshold
hyperpolarization
increases charge separation, more negative
What creates resting potential
Na/K pump, leak channels, ions. Ions are attracted to + outside or - inside. chemical forces move towards lower concentration
Na K pump
2 K in, 3 Na out
Saltatory conduction
jumping from node to node, no smooth conduction
What conducts action potential fastest
larger diameters (less resistance), more myelin (reduced capacitance- less ions to change potential)
methods to remove neurotransmitters
diffusion, degrade via enzymes, reuptake channels, astrocytes (pump out and break down)
How sarcolemma works with action potential
Action potential depolarizes sarcolemma, casuses SR to release Ca and bind troponin to make myosin move
Smooth muscle
hollow organs, blood vessels, involuntary, slow, 1 nuclei
Cardiac muscle
involuntary, branched, 1-2 nuclei, striated
Skeletal muscle
voluntary, fast, straight, many nuclei, striated
Type 1 muscle fibers
more mitochonria, red, slow contraction and conduction, aerobic, longer contraction, stronger, store triglycerides
Type 2 muscle fibers
white, fast contraction and conduction, anaerobic, short contraction, easily fatigued, ATP and creatine
Autonomic NS hormones
sympathetic (epi, norepi) and parasympathetic (ach)
Types of bone marrow
red bone barrow -hematopoeisis, yellow bone marrow- fat storage
trabecula
spongy bone
lacunae
space for bone cells, exchange nutrients
osteons
compact bone, cocentric layers of lamellae
osteoblasts
synthesis of collagen, proteins, form osteocytes and bone- grow and heal (less ca/ phosphate in blood)
osteoclasts
in lacunae, break down, works with phosphatase (MORE CA/PHOSPHATE IN BLOOD)
What maintains bone
PTH, calcitrol (D) increase Ca/P in blood and decrease in bone, and calcitonin decreases Ca/P in blood
Cartilage
form condrocytes, collagen, and elastin. Not innervated
Hyaline
Cartilage that reduces friction and absorbs shock
elastic Cartilage
shape, support
Fibrous cartilage
joints, rigidity, absorb shock
Tendons
muscle to bone
ligaments
bone to bone
pituatary gland
master gland, direct stimulation to other glands
thyroid
regulated by pituatary, regulates metabolism through T3 and T4
Parathyroid
regulates blood calcium and PTH
adrenal medulla
inner, makes catecholamines (epi/nor epi) - adrenaline
adrenal cortex
outer, makes steroids (cortisol and aldosterone which regulate blood volume)
Anterior pituitary
communicates with hypothalamus using hypophyseal portal
Hormones secreted by hypothalamus that signal pituatary
GnRH, CRH, TRH, GHRH, prolactin IF
What does GnRH release cause
release of FSH and LH from AP, which moves to gonads
What does prolactin IF do
Release from hypothalamus stimulates no prolactin
tropic hormones
FLAT- FSH, LH, ACTH, TSH- these stimulate glands
Direct hormones
PEG- prolactin, endorphins, GH
Posterior pituitary
stimulates using nerves to cause release of hormones that are made in hypothalmus - ADH and oxytocin
Protein hormones
receptors are in or on cell surface, secondary messengers
Steroid hormones
from lipids, receptors in cell. Primary messengers
tyrosine derivatives
thyroid, catecholamines
insulin
increase glucose storage, comes from Beta cells
glycolysis
irreversible, glucose to ATP
glycogenesis
formation of glycogen, reversible
lipogenesis
making lipids/ fatty acids. Irreversible, long term storage
glucagon
release glucose from storage, from alpha cells
gluconeogenesis
use amino acids to make glucose, reversible
glucogenolysis
break down glycogen, reversible
Ketogenesis
use fatty acids to make ketone bodies- starvation
islet of langergans
in pancreas. Alpha and beta cells make glucagon and insulin. Activated by calcium receptors
type 1 diabetes
pancreas does not produce insulin properly, glucose cannot enter cell
type 2 diabetes
insulin receptors do not work, glucose cannot enter cell
leptin
satiety hormone from hypothalamus, stimulated by insulin release
IVC
blood into heart from the body
Aorta
pumps blood out of the heart to body
3 layers of heart
endocardium, myocardium (contracts), pericardium
Pathway of blood
SVC/IVC to RA to RV to lungs and pulmonary system to LA to LV to aortic valve and aorta to body then back to SVC
Atrioventricular valves
tethered to walls, - triscupid (Between RA and RV), and mitral (between LA and LV)
What affects blood flow
length, radius, resistance
chordae tendonae
keep the valve from flipping backwards, keeps blood moving in one direction- works with papillary muscles
Interventricular septum
between ventricles, issues at membranous cause VSD
How deos the heart get blood and oxygen
coronary vessels
How does the lungs get oxygenated blood
bronchial vessles
Do RBC use oxygenated blood?
No, they have no mitochondira so they do not use oxygen
Systole
force of blood exiting the heart and going into the arteries during contraction
Diastolic
heart is relaxed, filling with blood
layers of blood vessel
tunica intima (basement membrane of endothelial cells), media (smooth muscle), and externa (collagen)
pulmonary artery vs. veins
pulmonary artery carries deoxygenated blood to lungs, pulmonary veins carries oxygenated blood to LA from lungs
artery
blood away from heart
vein
blood towards the heart