Biology Flashcards

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1
Q

What makes primary structure of proteins?

A

peptide bonds

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2
Q

What holds secondary structure of proteins?

A

folded chains (beta sheets and alpha helix) made of hydrogen bonds

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3
Q

What holds tertiary structure of proteins?

A

3D folded, hydrogen bonds, ionic bonds, dipole, dispersion, and disulfide

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4
Q

What holds quarternary structure of proteins?

A

multiple peptide subunits held by disulfide bonds

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5
Q

Which amino acids are important in alpha helix?

A

Proline and glycine- they destabilize alpha helix

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6
Q

Targets of phosphorylation

A

serine, threonine, tyrosine (Have OH nucleophile)

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7
Q

Which amino acids can mimic phosphate groups

A

asparatic and glutamic acid (bulky, negative)

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8
Q

What is a salt bridge?

types of bonds?

A

base and acid interact with hydrogen bonds and electrostatic interactions

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9
Q

Apoprotein vs. haloprotein

A

apoprotein is without prosthetic group, haloprotein has prosthetic

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10
Q

Isoelectric effect

A

pH when net charge on protein is zero, high pKa= high pH

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11
Q

Isoelectric focusing

A

separates protein based on difference in charge. They migrate until pH=pI (until neutral), uses a pH gradient, (-) cathode, and (+) anode

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12
Q

How does SDS work

A

denatures and gives uniform charge to seperate proteins by mass

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13
Q

How does Native Page work

A

Separates proteins based on size and charge … retains structure (no denaturing)

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14
Q

How is BME used in SDS page?

A

Breaks down disulfide bonds, is a reducing agent

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15
Q

Myosin

A

thick filament, muscle contraction. Uses ATP to crawl along actin

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16
Q

Kinesin

A

Aids movement of chromosomes and vesicles - intracellular transport

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17
Q

Dynein

A

movement of cilia and flagella in microtubules - intracellular transport and motility

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18
Q

What types of cells are pluripotent vs. multipotent

A

embryonic is pleuripotent, adult is multipotent

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19
Q

What crosses membrane?

A

Small, non-polar molecules

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20
Q

What makes a phospholipid

A

phosphate head, glycerol backbone, and fatty acid tail

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21
Q

What is hydrostatic pressure

A

pressure of liquid on container, reflects volume in a space

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22
Q

What is osmotic pressure?

A

Pressure required to prevent movement across semi-permeable membrane. Shows protein content of blood

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23
Q

Symport

A

couple energy to move in same direction (one down gradient, other against)

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24
Q

Secondary active transport

A

indirectly uses energy to move

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25
Q

Intercalated discs

A

In cardiac muscle, permits passage of ions between cells- allows rapid spread of action potential during contractions

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26
Q

Desosomes

A

connections between cells that ions/water can flow between. In the skin or intestine

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27
Q

Gap junctions

A

Tunnels between cells, allows ions/water to flow. Common in cells that use electrical coupling (cardiac, neurons)

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28
Q

Enzyme linked receptor

A

receptor tyrosine kinase, triggered by signals from the enviornment

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29
Q

Receptor tyrosine kinase

A

phosphorylates, acts as a dimer that crosslinks when ligand binds. Cross link activates other side. Regulates signal transduction and gene transcription

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30
Q

Smooth ER

A

lipid production, detoxifies, metabolizes carbs

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31
Q

Rough ER

A

protein production; exports out of cell and some modification

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32
Q

Golgi Apparatus

A

sorts, packages, protein modification, exports

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33
Q

Peroxisome

A

lipid destruction, contains oxidative enzymes, isolate peroxide and break it down

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34
Q

Lysosome

A

protein destruction, acidic enviornment for enzymes (protects cells- won’t work in cytoplasm)

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35
Q

Eukaryote vs. prokaryote

A

eukaroyotes compartmentalize, have membrane bound organelles, mitosis, nucleus. Prokaryotes divide by binary fission

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36
Q

Nucleolus

A

has DNA that makes rRNA. Ribosomes move through pore to cytoplasm

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37
Q

Mitochondira

A

make ATP, outer membrane with lipid bilayer and inner membrane with cristae… inter membrane is between outer and inner

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38
Q

Reactions and locations of cellular respiration

A

glycolysis: 6C to 2 3C pyruvate (cytoplasm), PDH complex (pyruavte to acetyl-CoA in matrix), Krebs uses acetyl coA to make NADH, FADH2 in matrix, and ETC which uses electron carrier to make ATP in the inner membrane

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39
Q

Microtubules

A

mitotic spindle, cilia, and flagella. has 9+2 arrangement

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40
Q

Intermediate filaments

A

permanent, has many proteins. Aids structural support, and resists stress/ compression

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41
Q

Microfilaments

A

in cytoplasm, made of actin. Aids movement and cell division. Can lengthen and shorten to move cell

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42
Q

4 parts of cell theory

A

cell is the basic unit, cells come from pre-existing cells, all living things are composed of cells, cells have DNA

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43
Q

Protists

A

eukaryote, unicellular, have nucleus and exist in moist enviornment, Not plant/fungus/animal

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44
Q

Archaea

A

prokaryotes, extreme enviornments (heat, salt, gas) -different cell wall

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45
Q

Bacteria

A

capsule, cell wall, membrane, flagella, nucleoid, pilli. NO NUCLEUS, circular dsDNA, some have plasmid

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46
Q

gram positive bacteria

A

purple, has membrane and thick peptidoglycan, capsule

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47
Q

gram negative bacteria

A

red, has inner membrane, outer membrane, thin peptidoglycan, lipopolysaccharide, capsule- More layers so purple washed off due to thin peptidoglycan and high fatty acid

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48
Q

Bacilli

A

Rod

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49
Q

Viruses

A

capsid (protein coat), and either ssDNA or RNA - no organelles, small, need host

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50
Q

bacteriorphage

A

viruses that infect bacteria, inject phage and use receptors or fuse with membrane

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51
Q

lytic

A

take over cells machinery to make copies, form functional viruses and then lyse cell to release- FAST but kills host

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52
Q

lysogenic

A

sneaks in, combine with hosts genetic information. Repressed, then replicates with bacteria. Provirus

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53
Q

retrovirus

A

enveloped, use reverse trancriptase to converse RNA to DNA to integrate into host genome

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54
Q

viroid

A

ss Circular RNA, infects plants. Self cleaves to reproduce

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55
Q

prions

A

infectious proteins, no genetic material- usually beta sheet

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56
Q

Mitosis checkpoints

A

G1/S and G2/M

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57
Q

G1 of mitosis

A

makes organelles, proteins, cyclines

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58
Q

G2 of mitosis

A

makes microtubules

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59
Q

Zona pellucida

A

outside of egg cell

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60
Q

What derives from endoderm

A

GI, lungs, liver, pancreas, stomach, SI, LI

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61
Q

What derives from mesoderm

A

skin, muscles, bone, kidney, bladder, ovaries

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62
Q

What derives from ectoderm

A

outer skin, sweat glands, hair, NS

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63
Q

stem cells

A

self renew, can differentiate into specialized cells if embryonic, somatic SC are used for repair

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64
Q

pluripotent stem cells

A

can differentiate into several types of cells

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65
Q

epithelial stem cells

A

can only form epithelial cells (utipotent)

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66
Q

hematopoietic stem cells

A

gives rise to many types of cell but in one family (multipotent)

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67
Q

Asymmetric replication of stem cells

A

form mother cell (identical) and daughter cell which is differentiated and becomes more specialized

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68
Q

Symmetric replication of stem cells

A

form two identical cells

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69
Q

induced pluripotent stem cells

A

revert back into pluripotent and can replace damage

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70
Q

Paracrine signalling

A

nearby (neurotransmitter, histamine)

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71
Q

Endocrine signalling

A

signal to large group using blood

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72
Q

Autocrine signalling

A

signal to site on the same cell

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73
Q

Juxtacrine signalling

A

signals nearbycells that are in direct contact

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74
Q

Hayflick limit

A

how many times a cell can divide before senescence (about 60)

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75
Q

Capases role in apoptosis

A

activates by cytochrome C, break down proteins after aspartate in apoptosis

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76
Q

gray matter

A

neurons somas, deep in spine, outer of brain

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77
Q

white matter

A

myelinated axons, deep in brain, outer of spine

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78
Q

cerebellum

A

coordinates movement, position, motor plan

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79
Q

brain stem

A

midbrain, pons, medulla, reticular formation - neurons, autonomic, control, cognition, emotion

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80
Q

Corpus callosum

A

travel between hemispheres

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81
Q

Basal ganglia

A

motor function, cognition, emotion

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82
Q

Thalamus

A

sensory, higher brain function

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83
Q

hypothalamus

A

controls pituatary gland, endocrine, higher functioning, makes ADH and oxytocin

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84
Q

Cerebral Cortex

A

gyri, sulci, fissures, frontal, parietal, temporal … has sensory, motor, and association areas

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85
Q

Broca vs. Wernicke

A

Broca is speech production, Wernickes is word meaning

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86
Q

CT scan

A

X ray to image brain

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87
Q

MRI

A

radio waves and magnetic field causes atoms to align and signal

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88
Q

EEG

A

electro activity of brain via electrodes- shows engagement

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89
Q

MEG

A

electrical currents in the brain

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90
Q

fMRI

A

functional MRI, shows active structures, firing means more oxygenated

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91
Q

PET scan

A

CAT + MRI, inject glucose, and shows active areas

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92
Q

Autonomic NS

A

unconcious, efferent neurons control smooth, cardiac, muscle, and gland cells

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93
Q

Dendrites

A

how information comes in, then goes to axon as graded potential

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94
Q

Afferent neurons

A

sensory, periphery to CNS

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95
Q

Efferent neurons

A

motor or autonomic, away from CNS to periphery

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96
Q

Astrocytes

A

glial cells of CNS, make scars, homeostasis, BBB, clean synapse

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97
Q

microglia

A

glial cells of CNS, secrete cytotoxic cells (phagocytosis) and antigen presentation

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98
Q

ependymal cells

A

glial cells of CNS, barrier between cerebrospinal and interstitial fluid. Helps move fluid

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99
Q

Oligodendrocytes

A

glial cells of CNS, create myelin sheath and aid exchange between cells

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100
Q

Schwann cells

A

glial cells of PNS from neural crest, support nerve cells and create myelin- only one axon

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101
Q

Depolarization

A

excitatory, move towards threshold

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102
Q

hyperpolarization

A

increases charge separation, more negative

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103
Q

What creates resting potential

A

Na/K pump, leak channels, ions. Ions are attracted to + outside or - inside. chemical forces move towards lower concentration

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104
Q

Na K pump

A

2 K in, 3 Na out

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105
Q

Saltatory conduction

A

jumping from node to node, no smooth conduction

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106
Q

What conducts action potential fastest

A

larger diameters (less resistance), more myelin (reduced capacitance- less ions to change potential)

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107
Q

methods to remove neurotransmitters

A

diffusion, degrade via enzymes, reuptake channels, astrocytes (pump out and break down)

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108
Q

How sarcolemma works with action potential

A

Action potential depolarizes sarcolemma, casuses SR to release Ca and bind troponin to make myosin move

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109
Q

Smooth muscle

A

hollow organs, blood vessels, involuntary, slow, 1 nuclei

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110
Q

Cardiac muscle

A

involuntary, branched, 1-2 nuclei, striated

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111
Q

Skeletal muscle

A

voluntary, fast, straight, many nuclei, striated

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112
Q

Type 1 muscle fibers

A

more mitochonria, red, slow contraction and conduction, aerobic, longer contraction, stronger, store triglycerides

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113
Q

Type 2 muscle fibers

A

white, fast contraction and conduction, anaerobic, short contraction, easily fatigued, ATP and creatine

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114
Q

Autonomic NS hormones

A

sympathetic (epi, norepi) and parasympathetic (ach)

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115
Q

Types of bone marrow

A

red bone barrow -hematopoeisis, yellow bone marrow- fat storage

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116
Q

trabecula

A

spongy bone

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117
Q

lacunae

A

space for bone cells, exchange nutrients

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118
Q

osteons

A

compact bone, cocentric layers of lamellae

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119
Q

osteoblasts

A

synthesis of collagen, proteins, form osteocytes and bone- grow and heal (less ca/ phosphate in blood)

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120
Q

osteoclasts

A

in lacunae, break down, works with phosphatase (MORE CA/PHOSPHATE IN BLOOD)

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121
Q

What maintains bone

A

PTH, calcitrol (D) increase Ca/P in blood and decrease in bone, and calcitonin decreases Ca/P in blood

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122
Q

Cartilage

A

form condrocytes, collagen, and elastin. Not innervated

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123
Q

Hyaline

A

Cartilage that reduces friction and absorbs shock

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124
Q

elastic Cartilage

A

shape, support

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125
Q

Fibrous cartilage

A

joints, rigidity, absorb shock

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126
Q

Tendons

A

muscle to bone

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127
Q

ligaments

A

bone to bone

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128
Q

pituatary gland

A

master gland, direct stimulation to other glands

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129
Q

thyroid

A

regulated by pituatary, regulates metabolism through T3 and T4

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130
Q

Parathyroid

A

regulates blood calcium and PTH

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131
Q

adrenal medulla

A

inner, makes catecholamines (epi/nor epi) - adrenaline

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132
Q

adrenal cortex

A

outer, makes steroids (cortisol and aldosterone which regulate blood volume)

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133
Q

Anterior pituitary

A

communicates with hypothalamus using hypophyseal portal

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134
Q

Hormones secreted by hypothalamus that signal pituatary

A

GnRH, CRH, TRH, GHRH, prolactin IF

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135
Q

What does GnRH release cause

A

release of FSH and LH from AP, which moves to gonads

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136
Q

What does prolactin IF do

A

Release from hypothalamus stimulates no prolactin

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137
Q

tropic hormones

A

FLAT- FSH, LH, ACTH, TSH- these stimulate glands

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138
Q

Direct hormones

A

PEG- prolactin, endorphins, GH

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139
Q

Posterior pituitary

A

stimulates using nerves to cause release of hormones that are made in hypothalmus - ADH and oxytocin

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140
Q

Protein hormones

A

receptors are in or on cell surface, secondary messengers

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141
Q

Steroid hormones

A

from lipids, receptors in cell. Primary messengers

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142
Q

tyrosine derivatives

A

thyroid, catecholamines

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143
Q

insulin

A

increase glucose storage, comes from Beta cells

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144
Q

glycolysis

A

irreversible, glucose to ATP

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145
Q

glycogenesis

A

formation of glycogen, reversible

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146
Q

lipogenesis

A

making lipids/ fatty acids. Irreversible, long term storage

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147
Q

glucagon

A

release glucose from storage, from alpha cells

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148
Q

gluconeogenesis

A

use amino acids to make glucose, reversible

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149
Q

glucogenolysis

A

break down glycogen, reversible

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150
Q

Ketogenesis

A

use fatty acids to make ketone bodies- starvation

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151
Q

islet of langergans

A

in pancreas. Alpha and beta cells make glucagon and insulin. Activated by calcium receptors

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152
Q

type 1 diabetes

A

pancreas does not produce insulin properly, glucose cannot enter cell

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153
Q

type 2 diabetes

A

insulin receptors do not work, glucose cannot enter cell

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154
Q

leptin

A

satiety hormone from hypothalamus, stimulated by insulin release

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155
Q

IVC

A

blood into heart from the body

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156
Q

Aorta

A

pumps blood out of the heart to body

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157
Q

3 layers of heart

A

endocardium, myocardium (contracts), pericardium

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158
Q

Pathway of blood

A

SVC/IVC to RA to RV to lungs and pulmonary system to LA to LV to aortic valve and aorta to body then back to SVC

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159
Q

Atrioventricular valves

A

tethered to walls, - triscupid (Between RA and RV), and mitral (between LA and LV)

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160
Q

What affects blood flow

A

length, radius, resistance

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161
Q

chordae tendonae

A

keep the valve from flipping backwards, keeps blood moving in one direction- works with papillary muscles

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162
Q

Interventricular septum

A

between ventricles, issues at membranous cause VSD

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163
Q

How deos the heart get blood and oxygen

A

coronary vessels

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164
Q

How does the lungs get oxygenated blood

A

bronchial vessles

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165
Q

Do RBC use oxygenated blood?

A

No, they have no mitochondira so they do not use oxygen

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166
Q

Systole

A

force of blood exiting the heart and going into the arteries during contraction

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167
Q

Diastolic

A

heart is relaxed, filling with blood

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168
Q

layers of blood vessel

A

tunica intima (basement membrane of endothelial cells), media (smooth muscle), and externa (collagen)

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169
Q

pulmonary artery vs. veins

A

pulmonary artery carries deoxygenated blood to lungs, pulmonary veins carries oxygenated blood to LA from lungs

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170
Q

artery

A

blood away from heart

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171
Q

vein

A

blood towards the heart

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172
Q

pulmonary artery

A

deoxygenated blood to lungs

173
Q

pulmonary vein

A

oxygenated blood from lungs to heart

174
Q

What makes up plasma

A

water, proteins (albumin, antibody, fibrinogen), hormones, electrolytes, nutrients

175
Q

What makes up blood

A

Plasma, WBC, platelets, RBC, HGB

176
Q

hematocrit

A

volume of RBC/ total volume

177
Q

aveolus

A

where oxygen interfaces with blood vessels

178
Q

What aids blood clots

A

collagen, platelets, fibrin, and thrombin. Tissue factors tell when to clot

179
Q

antithrombin

A

plasmin degrades clot

180
Q

thrombin

A

facilitates fibrinogen into fibrin for blood clots

181
Q

What stimulates RBC production

A

erythropoeitin, released by kidney when oxygen is low

182
Q

Bohr effect

A

CO2 and hydrogen affects the affinity of HGB for oxygen

183
Q

Haldane effect

A

O2 affects the affinity of HGB for CO2 and hydrogen

184
Q

Universal donor vs. acceptor

A

donor is O, acceptor is AB

185
Q

Role of spleen

A

recognizes old RBC and degrades them using monocytes

186
Q

What are pluripotent hematopoietic cells

A

Can make any type of blood cell (myeloid or lymphoid)

187
Q

Myeloid

A

forms RBC, mega karyocytes, monocytes, neurtophils, basophils, eosinophils, and mast cells

188
Q

Mega karyocytes

A

make platelets

189
Q

monocytes

A

form macrophage

190
Q

mast cells

A

function in allergic reactions, make histamine

191
Q

lymphoid cells

A

form lymphocytes (B and T cells) and NK cells

192
Q

Which muscles contract during inhalation

A

external intercostal, and diaphragm - which flattens for expansion

193
Q

Elastin

A

surrounds alveoli to allow for expansion when breathing

194
Q

What receptors are used in the respiratory center

A

central chemoreceptors (CO2/pH), peripheral chemoreceptors (O2, CO2, pH), mechanoreceptors (pressure)

195
Q

respiratory center

A

uses motor nerves to control diaphragm, intercostal muscles, abdominal, and accessory muscles

196
Q

MHC-2

A

senses infections, activate helper T cells by presenting antigens at surface of cell (uses peices that were phagocytized)

197
Q

phagocytes

A

neurophils (fast), macrophage, dendritic cells

198
Q

Dendritic cells

A

antigen presenting cells, have MHC-2

199
Q

Cells of specific immunity

A

B and T cells

200
Q

B lymphocytes

A

Hummoral response, make antibodies and present on surface to mark for destruction. Bind specific antigens to be activated

201
Q

How are self responding immune responses prevented

A

B cells removes self responding antibodies from the surface so that they only respond to foreign. Kill cells that self react

202
Q

What do B lymphocytes become

A

memory or effector cells

203
Q

T lymphocytes

A

Cell mediated, helper or cytotoxic T cells

204
Q

Helper T cells

A

bind certain antigen presenting cells, (from MHC-2) activates, and proliferates to become effector or memory T cells

205
Q

Effector helper T cells

A

release cytokines and gets cell ready for immune response. Activates B cells.

206
Q

Cytotoxic T cells

A

kills other cells that are infiltrated. MHC 1, differentiates into memory and effector

207
Q

MHC-1

A

on all nucleated cells in the body, MHC presents problems in cells to cytotoxic to kill

208
Q

CD4+

A

binds MHC-1, mostly helper T

209
Q

CD8+

A

binds MHC-2, cytotoxic

210
Q

Clonal selection

A

preparing B and T cells to fight. Cells wait in lymph nodes form something to react. Select out for whats needed for response and clone

211
Q

Non specific immune response

A

phagocytes, WBC, leukocytes, inflammatory, barriers

212
Q

small intestine

A

hydrolysis and absorption

213
Q

large intestine

A

absorption (water, ions, vitamins)

214
Q

lingual lipase

A

breaks down triglycerides in mouth into free fatty acids

215
Q

alpha amylase

A

breaks down carbs to smaller carbs

216
Q

Cells of stomach

A

parietal cells, cheif cells, mucous cells

217
Q

Parietal cells

A

releases HCl

218
Q

cheif cells

A

secrete pepsinogen to degrade proteins

219
Q

mucous cells

A

secrete mucous to coat stomahc

220
Q

duodenum

A

recieves chyme, digests. has villi and bile from the liver/GB to emusify fat

221
Q

pancreas enzymes

A

trypsinogen, chymotrypsin, amylase, lipase, enteropeptidase

222
Q

enteropeptidase

A

makes active forms of enzymes (trypsin, chymotrypsin)

223
Q

brush boarder enzymes

A

peptidase, lactase, nucleosides

224
Q

nucleoside

A

sugar and base - no phosphate

225
Q

Jejunum

A

most absorption, bile to GI tract

226
Q

ileum

A

absorption, bile/fat absorbed and moves to liver to repeat - connects to cecum

227
Q

liver

A

metabolism, storage of nutrients (carbs, fats), moves proteins to blood, detoxifies, makes bile

228
Q

How does the liver get products from the blood?

A

portal vein, hepatic artery, hepatic vein, common hepatic duct, hepatic triad

229
Q

portal vein

A

gives liver nutrient rich blood

230
Q

hepatic artery

A

gives liver oxygen rich blood

231
Q

hepatic vein

A

nutrient and oxygen poor blood leaves liver and goes to heart

232
Q

common hepatic duct

A

allows bile to leave the liver and move to cystic duct to gallbladder

233
Q

hepatic triad

A

portal bein, hepatic artery, common hepatic duct

234
Q

bile

A

pigments, salts- help emulsify fat to micelles. Stored in Gallbladder

235
Q

CCK

A

causes gallbladder to contract and release bile. uses common bile duct to move to small intestine.

236
Q

Pancreas

A

releases enzymes to duodenum, amylase and lipase, proteolytic enzymes, hormones

237
Q

How is the stomach neutralized

A

pancreas releases Bicarb to neutralize the HCl

238
Q

Proleolytic enzymes

A

trypsinogen (activated by enteropeptidase), and chymotrypsin (Activated by trypsin)

239
Q

Delta islet cells

A

in pancreas, release somatostatin- stops active hormones

240
Q

large intestine structure

A

cecum to ascending, transverse, descending, and sigmoid colon. Asb

241
Q

Large intestine sphincters

A

external is skeletal, internal is smooth muscle

242
Q

What controls GI

A

Enteric NS, hormones (gastrin), secretin

243
Q

Gastrin

A

released from stomach and stimulates digestive juices (HCl, pepsinogen) to release. Decreases when pH is low.

244
Q

Secretin

A

released when acidic chyme moves to duodenum. Stimulates pancreas to release bicarb, and fat in chyme causes CCK from SI

245
Q

CCK in the stomach

A

moves to pancreas to release lipase. And contraction/ bile release and slowing down stomach motility

246
Q

Epidermis layers

A

epitheleal tissue. Has basale, spinosum, granulosum, lucidum, and corneum.

247
Q

Basale

A

Deepest layer of skin, has keratinocytes, melanocytes, rapid division

248
Q

Spinosum

A

desosomes, langerhans (immune)

249
Q

Granulosum

A

granules, and lamellar body (makes lipids)

250
Q

lucidum

A

dead keratinocytes, clear

251
Q

Corneum

A

top, lipid (water proof), dead

252
Q

Dermis

A

Connective tissue. Has collagen and actin. Papillary and reticular layers

253
Q

Papillary dermis

A

top, thin, loose. Has blood vessels and nerve endings

254
Q

reticular dermis

A

deeper, thicker, denser. Glands, follicles, arrector pilli muscle (goose bumps) COLLAGEN

255
Q

hypodermis

A

bottom, subcutaneous fat (absorbs shock, insulation)

256
Q

Sweat

A

sebum, apex secretion (protein, lipids, steroids), watery

257
Q

meissners receptor

A

mechanoreceptor, light touch, in papillary, non hairy

258
Q

pacinian receptor

A

layered, deep touch, hypodermis

259
Q

Merkels

A

in between stratum and papillary, sustained light touch

260
Q

Ruffini receptor

A

sustained touch, needs collagen to work- in reticular dermis

261
Q

Hair receptor

A

triggered by deflected hair. Light touch, reticular dermis

262
Q

Nociceptor

A

pain

263
Q

TrpV1 receptor

A

temperature and pain

264
Q

epididymis

A

sperm development (gain mobility) then move to vas deferens and urethra

265
Q

seminal vesicles

A

contributes fluid to semen

266
Q

semen

A

alkaline, semen and seminal fluid, sugar for ATP

267
Q

seminiferous tubules

A

where sperm is made and leaves in testes

268
Q

sertoli cells

A

spem develop

269
Q

stages of sperm

A

spermatogonium, primary spermatocyte, seconday spermatocyte, spermatoza, sperm

270
Q

testosterone

A

binds genes to influence function, made in leydig cells. Convert some to estrogen, other to DHT. regulated by DHT and keeps sperm production going

271
Q

oogenesis

A

oogonia divide to make primary oocyte

272
Q

Secondary oocyte

A

made at puberty - in meiosis, one takes cytoplasm, other is polar body

273
Q

Fertilization

A

sperm fuses with egg in meiosis 2, oocyte makes another polar body and becomes Ovuum, secondary is mestruated out

274
Q

Follicles

A

where primary egg develops, has granulosa cells to secrete hormone. Dominant follice is ovulated

275
Q

primary follucles

A

Pellucida separates granulosa and eggs. gap junctions allow granulosa to nourish egg.

276
Q

Corpus luteum

A

FSH- aids follicle maturation

277
Q

Estrogen and progesterone

A

gets follicles ready for implantation

278
Q

Menopause

A

loose ability to respond to LH/FSH

279
Q

Umbilical artery vs. vein

A

umbilical artery is away from heart, vein is towards

280
Q

Trophoblasts

A

aids diffusion of nutrients between mom and fetus blood cells

281
Q

Estrogen

A

by ovaries, transported via blood and binds to change expression.

282
Q

What causes estrogen release

A

FSH causes ovaries to develop and granulosa releases estrogen

283
Q

Labor

A

progesterone drops so muscles relax. More sensitive to other hormones allows for contraction. Oxytocin from PP causes contraction

284
Q

Lactation

A

via lactiferous ducts, PIF is turned off in hypothalamus so that AP can release prolactin.

285
Q

Suckling

A

hypothalamus stimulates release of oxytocin. More sucking causes more oxytocin release and more milk release

286
Q

lyase

A

breaks bond without using water

287
Q

What breaks down enzyme complex

A

Heat affects non covalent interactions (such as active site), or alter pH

288
Q

founder effect

A

move to new population, less variation and more drift

289
Q

Bottleneck effect

A

most of population is killed by stress

290
Q

Coevolution

A

two species evolve alongside eachother and exert pressures on eachother

291
Q

Gene flow

A

alterations in composition of gene pool due to migration

292
Q

genetic drift

A

changes in frequency of gene pool due to chance

293
Q

catabolism vs. anabolism

A

catabolism is breaking down, anabolism is forming

294
Q

albumin

A

allows fatty acids to travel in the blood stream

295
Q

hormone sensitive lipase

A

breaks down fat when levels of glucagon or insulin change

296
Q

immunohistochemistry

A

uses antibodies to check for tissue markers - identify antigens using antibody binding

297
Q

Cellular respiration

A

Glycolysis (anaerobic), Krebs (aerobic), ETC

298
Q

when to use gluconeogenesis

A

when fasting- need to pump glucose into blood

299
Q

functions of NADPH

A

cholesterol and fatty acidy synthesis, protect from ROS

300
Q

Where does translation occur

A

cytoplasm

301
Q

Deoxy ribose

A

no 2’ oxygen

302
Q

Topoisomerase

A

unwinds DNA and prevents supercoiling

303
Q

Helicase

A

unwinds and breaks hydrogen bonds

304
Q

Eukaryotic vs. prokaryotic ribosomal subunits

A

Euk have 60 and 40s, 80s total. Pro have 50s and 30s, 70s total

305
Q

DNA polymerase 1

A

removes primer at the end of replication, exonuclease

306
Q

DNA polymerase 3

A

proofreads, exonuclease

307
Q

Mismatch repair

A

recognize, cut, econuclease removes nucleotide, DNA pol inserts the correct nucleotide and the ligase seals

308
Q

Nucleotide excision repair

A

endonuclease removes dimers/ structural errors (Does not need to be at the end), polymerase brings in new nucleotide, ligase seals

309
Q

How do bacteria distinguish parent strand

A

methylated is correct strand (parent)

310
Q

activator

A

enhance reaction between promoter and RNA polymerase

311
Q

Repressor

A

binds operator, inducer can cause repressor to detach from operator

312
Q

Silencer

A

repressor binds, RNA polymerase cannot bind promoter

313
Q

Splicesome

A

cleaves introns so that they stay in the nucleus using a hair pin

314
Q

snRPS

A

splicesome for introns in the mRNA, process pre RNA in the nucleus, regulates transcription factors and telomeres

315
Q

non conservative mutation

A

loss of function (sickle cell, glu to val)

316
Q

Missense mutation

A

changes amino acid

317
Q

Nonsense mutation

A

premature stop codon

318
Q

Conservative mutation

A

same type of amino acid

319
Q

silent mutation

A

different codon that codes for the same amino acid

320
Q

penetrance

A

fraction with a genotype that express a phenotype

321
Q

constant vs. variable expressivity

A

constant is expressed in the same way each time, variable has different phenotypes with same genotype

322
Q

How do antibiotics work

A

they reduce enzymes of bacteria and increase activation energy of bacteria

323
Q

Mg cofactor with DNA polymerase

A

works with DNA polymerase to bind negative DNA, bind at asparatate. Any changes in pH affect Mgs ability to bind (aspartate is negative at neutral pH)

324
Q

Synapsis

A

pairing of homologous chromosomes

325
Q

Endosymbiotic theory

A

mitochondria and chloroplasts were independent and joined together to become eukaryotic cell. Both help eachother

326
Q

Why are mitochrondrial disorders passed through the mother?

A

mitochondria replicated independent from the rest of the cell, and are passed from the mothers egg

327
Q

retroviruses

A

enveloped SS RNA with 3 proteins, use reverse transcriptase and exit nucleus like normal RNA to form proteins

328
Q

integrase

A

works with reverse transcriptase to remove 3’ ends to form sticky ends and integrate DNA to host for cirus

329
Q

Total lung capacity

A

max volume when one inhales completely

330
Q

Residual volume

A

minimum volume of air left in the lungs after one exhales completely

331
Q

Vital capacity

A

difference between minimum and max volume of air (TLC-RV)

332
Q

Tidal volume

A

amount of air inhaled or exhaled in a normal breathe

333
Q

Expiratory reserve volume

A

Amount of air the can be forcibly exhaled after a normal breathe

334
Q

Inspriratory reserve volume

A

Amount of air the can be forcibly inhaled after a normal breathe

335
Q

What regulates respiratory?

A

Medulla. Chemoreceptors respond to blood CO2 and O2 and cerebrum (voluntary)

336
Q

Functions of lymphatic system

A

Removes interstitial fluid from tissues into lymph, absorbs and transports fats from the intestine, helps mature lymphocytes, brings molecules that leak into the interstitial system back into the blood

337
Q

Vasa recta

A

in kidney, gives oxygen

338
Q

Kidney

A

Filters blood and makes urine, has 2 capillary beds: vasa recta, peritubular capillaries

339
Q

Peritubular capillaries

A

collect nutrients for the kidneys to filter

340
Q

Renal artery

A

helps kidney take in blood

341
Q

Kidney filtration

A

filters waste and saves whats important - at glomerulus

342
Q

Kidney collection

A

Collect and put out urine

343
Q

Kidney homeostasis

A

pH, bp, osmolarity, waste

344
Q

Nephron

A

Filtration, collection. Has outer (renal cortex) and inner (renal medulla)

345
Q

Renal calyx

A

collects urine in kidney, collects into renal pelvis, and exits through ureter to bladder

346
Q

Renal hila

A

two vessels leave kidney (artery, vein, ureter)

347
Q

Bowmans capsule

A

collects fluid from the glomerulus that is filtered from the blood.

348
Q

Bowmans space

A

where amino acids, glucose, ions leak from glomerulus to bowmans capsule. Has endothelial cells, podocytes, epitheleal cells

349
Q

Proximal convuluted tubule

A

reabsorbs amino acids, ions, glucose, and water from the glomerulus

350
Q

descending LOH

A

reabsorbs water

351
Q

Ascending LOH

A

reabsorbs ions

352
Q

How does the kidney use counter current multiplication

A

The medulla actively absorbs - is salty. Causes the descending LOH to passively absorb water

353
Q

Distal convulted tubulue

A

loops back from LOH to glomerulus, reabsorbs ions and nutrients

354
Q

Why can the LOH reabsorb urea

A

Absorbed by medulla to help drive water reabsorbtion by altering osmolarity

355
Q

Collecting duct

A

collects anything leftover in nephron, reabsorbs water and urine

356
Q

How does reabsorbed product reenter blood in kidneys

A

efferent arterioles collects and returns the reabsorbed nutrients to the blood and body, uses renal vein

357
Q

What lines the bladder

A

transitional epitheleal, allows for expansion

358
Q

Pathway of urine

A

renal calyces to renal pelvis to ureter to bladder to urethra

359
Q

Internal urethral sphincter

A

smooth muscle (involuntary) - makes sure bladder does not leak

360
Q

External urethral sphincter

A

voluntary control to urinate

361
Q

What triggers renin release

A

low BP, sympathetic responses, low salt in DCT via macula densa cells

362
Q

What secretes angiotensinogen

A

liver cells

363
Q

Angiotensinogen

A

activated by renin into angiotensin 1

364
Q

angiotensin 2

A

angiotensin 1 forms angiotensin 2 at endothelieal cells via ACE

365
Q

How does acetylation affect transcription

A

Acetyl groups weaken lysines on the histones. Causes less attraction to DNA. Making DNA more accessible to increase trx

366
Q

Transcription Initiation complex

A

RNA polymerase, promoter, TATA binding

367
Q

mRNA

A

template, carries code to cytosol for translation

368
Q

tRNA

A

carries triplet code to amino acid sequence

369
Q

Translation termination

A

stops codon reaches A site and release factors bind. Water adds and frees the polypeptide

370
Q

Hardy Weinberg Criteria

A

shows no change in allelle frequencies over time. No selection of fittest, has random mating, migration does not affect gene pool, large population, mutational equilibrium

371
Q

Non polar alkyl amino acids

A

GAPVLIM

372
Q

Non Polar Aromatic Amino Acids

A

phenylalanine, trp

373
Q

Polar neutral amino acids

A

S, threonine, Asp, glu, cys, tyr

374
Q

polar acidic amino acids

A

aspartic and glutamic acid

375
Q

Polar basic amino acids

A

has nitrogen, his, lys, arg

376
Q

Histidine

A

has pKa close to neutral, can be pronated or depronated

377
Q

Proline

A

secondary alpha amino, disrupts alpha helixs and makes kinks

378
Q

Glycine

A

not chiral, very flexible- disrupts alpha helix

379
Q

Cysteine

A

extracellular= oxidizing and forms disulfide bridges

380
Q

amino group of amino acids

A

proton accept, basic, pKa=9- Nucleophile

381
Q

secondary protein structure

A

alpha helix, beta sheet. Has hydrogen bonds

382
Q

tertiary protiein structure

A

hydrogen bonds, hydrophobic, vanderwalls, disulfide

383
Q

quartenary protein structure

A

hydrogen bonds, hydrophobic, vanderwalls, disulfide- between subunits

384
Q

How does temperature change affect proteins

A

changes folding (2,3,4)

385
Q

How does acidic affect proteins

A

changes ionic bonds (3,4)

386
Q

How do chemicals affect proteins

A

changes hydrogen bonds (all)

387
Q

How does enzyme affect proteins

A

between amino acids (1)

388
Q

prophase

A

condense, membrane dissolves, begin to migrate

389
Q

metaphase

A

membrane is gone, begin to line up, cetromeres are opposite with microtubules

390
Q

anaphase

A

microtubules pull (at kinetochore), 4 chromatids

391
Q

telophase

A

unwinds, furrows, membrane forms

392
Q

PCR

A

makes many copies of DNA using taq polymerase

393
Q

terpenes

A

lipids, 5 carbon isoprene, helps make steroids

394
Q

Z line

A

sacromere, anchors actin, shortens in contraction

395
Q

A band

A

Myosin and actin, no change in contraction

396
Q

I band

A

just actin, moves, gets smaller in contraction

397
Q

titin

A

elastic, attatches myosin to Z line

398
Q

PTH

A

secreted when blood calcium is low, stimulates osteoclasts to absorb more bone

399
Q

atypical genotypes

A

close together on loci, may be inherited as pair. No crossing over

400
Q

Fecundity

A

selected for, potential to bear children

401
Q

Calcitonin

A

secreted when blood calcium is high, decreases osteoclasts, decreases blood calcium

402
Q

Allosteric

A

reversible, non covalent

403
Q

DNA methylation

A

silences DNA, adds methyl to cytosine and adenine to tightly coil DNA and inactivate

404
Q

CpG islands

A

high Cytosine and guanine in the promoter regions, high methylation and deactylation are inactive

405
Q

Alternative splicing

A

one gene can code for multiple proteins when spliced differently

406
Q

Mitosis

A

uses divisions to mulitply, makes identical cells, BODY cells

407
Q

Meiosis

A

contributes to genetic variety, makes gametes. Is a reduction division (46 to 23 chromosomes)

408
Q

interphase

A

growth, replication, cell processes (still have 46 chromosomes but each has a pair… now have 92 chromatids)

409
Q

crossing over

A

in prophase 1, when homologous chromosomes are lined up and transfer/ exchange genetic information

410
Q

recombinant chromosomes

A

contributes to variety as a result of crossing over

411
Q

Metaphase Meiosis 1

A

chromosomes line up as 2 pairs in the middle. Each pair moves to one side

412
Q

prophase meiosis 1

A

homologous pairs, crossing over

413
Q

Products of meiosis part 2

A

4 cells

414
Q

Chromosome # before and after mitosis

A

46 paired, 92 chromatids before and 46 chromosomes, 46 chromatids per daughter after (each pair splits)

415
Q

Chromosome # before and after meiosis 1

A

46, 92 (like in interphase) to 23 chromosomes, 46 chromatids (each pair set splits)

416
Q

Chromosome # before and after meiosis 2

A

23 chromosomes, 23 chromatids (each single) in 4 cells

417
Q

diploid

A

2n, 2 SETS of chromosomes

418
Q

evolutionary game theory

A

competitive or cooperative strategy to maximize reproductive success and maximize fitness

419
Q

spermatids

A

formed after meiosis 2

420
Q

What is mRNA complementary to

A

anti coding

421
Q

How do rods work to process light?

A

turn off when light is present and causes cascade by turning on

422
Q

rhodopsin

A

in rod, has retinal

423
Q

retinal

A

light causes shape change (cis/bent to trans/straight), triggers rhodopsin to change shape

424
Q

transducin

A

shape change of rhodopsin causes transducin to break away and alpha subunit binds phosphodiesterase

425
Q

phosphodiesterase in phototransduction

A

converts cGMP to GMP

426
Q

GMP in phototransduction

A

GMP causes sodium chanells to close, hyperpolarization, and rods to turn off

427
Q

bipolar cells

A

rods off allows center bipolar cells to turn on, and signal optic nerve

428
Q

Hershey Chase experiement

A

pellet incorporated DNA, surfactant had external