biological molecule testing Flashcards
what agent do u use to test for starch
iodine
what agent do u use to test for a reducing sugar
benedict’s solution and heat for 5 mins at 80°C
what is a reducing sugar include
all monosaccharides and some disaccharides
what’s the test for non reducing sugars
add hydrochloride acid then sodium carbonate then benedict’s and heat for 5 mins at 80°C
what is the positive result for non reducing sugars
green-very low
yellow-low
brown- medium
brick red- high
what is the negative result for non reducing sugars
blue
what is the test for lipids (emulsion)
add ethanol then decant distilled water
what is the positive result for lipids
cloudy emulsion
what is the negative result for lipids
clear
what does a colorimeter test for
reducing sugars
how to use colorimeter
-shine light through sample
-use pipette to take super
what is the agent used to test for proteins
biuret solution
what is the positive test for proteins
turns from blue to purple