BIOL 200

Question 1

Light microscopy

Answer 1

PROS
- Live cells can be viewed and recorded.
- Specific structures can be tagged for viewing
CONS
- Limit of conventional resolution is 0.2 micro meters.

Question 2

Transmitted light microscopy.

Answer 2

Sample sits between light source and viewfinder, light seen is able to pass through sample.

Question 3

Emitted light microscopy

Answer 3

Illuminated by a light source off to the side, molecules get excited and release photons. Light comes from samples themselves. AKA Fluorescent light microscopy.

Question 4

Fluorescence microscopy

Answer 4

Cell illuminated with light of appropriate wave length to excite fluorochrome.

Question 5

Confocal Laser scanning microscopy

Answer 5

Fluorescence light microscopy, laser is used to scan the sample.

Question 6

Direct staining

Answer 6

Fluorochrome is combined with a macromolecule.

Question 7

indirect immunofluorescence staining

Answer 7

fluorochrome is covalently linked to antibodies.

Question 8

antibodies

Answer 8

protein complexes produced by mammals that bind to a protein or other macromolecule.

Question 9

Immunolabeling

Answer 9

Primary antibody is applied, specific to the antigen u want to see. A secondary antibody is applied to the conjugated fluorochrome, different animal than primary.

Question 10

Green fluorescent protein

Answer 10

small luminescent protein that was discovered in jellyfish.

Question 11

Electron microscopy.