Biochemistry-Pyruvate Oxidation_TCA Cycle Flashcards

1
Q

Why are beta-keto acids easier to decarboxylate that alpha-keto acids?

A

Beta-keto acids are resonance stabilized. Alpha-keto acids form an unstable carbanion.

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2
Q

Is pyruvate easy or difficult to decarboxylate?

A

Difficult it is an alpha-keto acid.

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3
Q

Pyruvate Decarboxylation Reaction

A

Done by pyruvate dehydrogenase complex. Vitamins are key coenzymes in carrying out this reaction.

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4
Q

Alcoholics and people with eating disorders cannot walk very well. What biochemical molecule is deficient and causes these symptoms?

A

Thiamine. It can cause polyneuropathy, paresthesia and paresis (partial paralysis) through nerve demyelination

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5
Q

What are the 5 P’s of thiamine deficiency?

A

Polyneuropathy, Paresthesia, Paresis, Psychiatric, Possible Edema

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6
Q

What makes thiamine so reactive?

A

Center carbon atom can be extracted to form a carbanion.

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7
Q

Why is thiamine a good coenzyme for decarboxylation of pyruvate?

A

Its attack on pyruvate’s ketone resembles a beta-keto acid which is easier to decarboxylate.

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8
Q

What portion of the pyruvate dehydrogenase complex would be lost in a patient with thiamine deficiency?

A

The E2 core.

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9
Q

alpha-keto acid dehydrogenase reaction sequences.

A

*

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10
Q

How does arsenic cause harm?

A

It blocks reoxidation of a disulfide group in pyruvate dehydrogenase complexes.

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11
Q

How is pyruvate made from acetyl-CoA?

A

It isn’t. It is an irreversible reaction in humans.

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12
Q

How is pyruvate dehydrogenase complex regulated?

A

E1 kinase phosphorylates complex and inactivates it. E1 phosphatase removes phosphate group and activates the complex. E1 kinase is inhibited by ADP and pyruvate. E1 phosphatase is stimulated by Ca.

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13
Q

What substances change pyruvate dehydrogenase complex conformation so it is more readily phosphorylated by E1 kinase?

A

Acetyl CoA and NADH.

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14
Q

What substances change pyruvate dehydrogenase complex conformation so it is more readily dephosphorylated by E1 phosphatase?

A

Pyruvate and NAD.

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15
Q

A parent brings her 2 year old in complaining of weakness, seizures, vomiting and opthalmoplegia. What syndrome might this child be suffering from? What could you give the patient to alleviate symptoms?

A

Leigh syndrome. Giving high levels of thiamine can compensate for malfunction in pyruvate dehydrogenase and decrease lactic acid levels in the blood.

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