Biochemical Test Flashcards
What is Benedict’s reagent
Blue solution, which is used to test for reducing a non-reducing sugars
What is biuret test?
Simple biochemical reaction to detect the present of protein turns purple when protein is present
What is the emulsion test?
Test for lipids, make sure sample with ethanol, and then add water if it turns white and cloudy lipid is present
What is a non-reducing sugar
A sugar, which cannot serve as reducing agent, e.g. sucrose
Non-reducing sugar test
Following a negative reducing sugars test heat the solution with HCL to hydrolyse the non-reducing sugar into its monosaccharides, then perform the Benedict test again if you get a positive result after hydrolysis there is nonreducing sugars is present
What is a reducing sugar?
A sugar that serves as a reducing agent or monosaccharides are reducing sugars, along with some disaccharides
