Biochem - Immobilized Enzyme

Question 1

1. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have

Answer 1

1. High molecular weight

Question 2

2. Functional groups of the nonessential amino acids that are suitable for the immobilization process are

Answer 2

2. All of the above

Question 3

3. Which medium is used for the production of Penicillin using immobilized cells?

Answer 3

3. Glucose medium

Question 4

4. Which of the following is taken as an assumption in the distributed model?

Answer 4

4. All of the above

Question 5

5. The immobilized enzyme produced by micro encapsulation technique provides

Answer 5

5. An extremely large surface area

Question 6

6. Which of the following is considered as a disadvantage to the adsorption method of immobilization?

Answer 6

6. State of immobilization is very sensitive to solution pH, ionic strength, and temperature

Question 7

7. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

Answer 7

7. Equal to that of substrate consumption

Question 8

8. The immobilized technique involving chemical method is

Answer 8

8. Covalent bond formation dependent

Question 9

9. For a steady state condition, the change of substrate concentration (dCSS​/dt) is

Answer 9

9. Zero

Question 10

10. Damkohler number (NDaDa​) is

Answer 10

10. The ratio of the maximum reaction rate to the maximum mass transfer rate

Question 11

11. For glucose isomerization by immobilized enzyme, the reactor generally used is

Answer 11

11. Packed Bed

Question 12

12. If Damkohler number, NDaDa​»1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csbsb​and Css​are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. kss​mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)

Answer 12

12. rP=kSa(Csb−Cs)rP​=kS​a(Csb​−Cs​)

Question 13

13. The most commonly employed cross-linked polymer is the

Answer 13

13. Polyacrylamide gel

Question 14

14. Which of the following is not the correct answer?

Answer 14

14. None of the above

Question 15

15. Commonly employed water insoluble supports the covalent attachment of enzyme include

Answer 15

15. All of these

Question 16

16. Which of the following is not a physical method of immobilization?

Answer 16

16. None of these

Question 17

17. The support material for immobilization of cells of Bacillus subtilis is

Answer 17

17. Agarose and carbodiimide

Question 18

18. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

Answer 18

18. All of the above

Question 19

19. The covalent attachment of enzyme molecules is via

Answer 19

19. Nonessential amino acids residues to water insoluble, functional supports

Question 20

20. To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, n can be defined as

Answer 20

20. Actual reaction rate/rate if not slowed by diffusion

Question 21

21. Which is not the method for producing immobilized enzymes with multifunctional reagents?

Answer 21

21. None of the above

Question 22

22. The immobilization technique involving physical method is

Answer 22

22. Non-covalent bond formation dependent

Question 23

23. which of the following is the commonly employed adsorbents?

Answer 23

23. All of these

Question 24

24. Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses

Answer 24

24. A diffusion cell with two compartments divided by a thin gel

Question 25

25. The effectiveness factor increases with the

Answer 25

25. Increase of diffusivity and decreased with the increase of particle size