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D2 > Biochem - Fermentation Kinetics [1] > Flashcards

Biochem - Fermentation Kinetics [1] Flashcards

1
Q
  1. The function of the disengagement zone in an airlift fermenter is to
A
  1. Prevent CO2 rich bubbles from entering the downcomer
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2
Q
  1. Stationary phase is described as
A
  1. No further increase in the cell population after a maximum value
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3
Q
  1. In the accelerated phase, cell starts to
A
  1. Increase and the division rate increases to reach a maximum
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4
Q
  1. The Monod model predicts that the specific growth rate
A
  1. Will increase with the concentration of the growth limiting substrate until it reaches a maximum value
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5
Q
  1. A mixed fermentation is one, which produces
A
  1. Both acid and alcohol
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6
Q
  1. The function of a mechanical seal is to
A
  1. Prevent contaminants entering the reactor
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7
Q
  1. The phenomenon in which substrates are used in a sequential manner is known as
A
  1. Diauxie
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8
Q
  1. The dilution rate, D is defined as
A
  1. F/VR
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9
Q
  1. Diauxie is
A
  1. The stagewise uptake of nutrients
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10
Q
  1. An open system in which the growth rate is maintained by adding a nutrient (present in limiting quantities) at the same rate as that medium containing micro-organism is removed is called
A
  1. Chemostat
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11
Q
  1. In the death phase
A
  1. Nutrients available for the cells are depleted and begin to die
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12
Q
  1. The maximum specific growth rate of an organism depends on
A
  1. All of these
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13
Q
  1. Which of the following is not correct for the Monod model and the Michaelis Menten Model
A
  1. The Michaelis Menten Model was derived from a curve fitting exercise
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14
Q
  1. Bubble column reactor has
A
  1. Large height to diameter ratio
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15
Q
  1. Why a T-flask used in small-scale cell culture is incubated in a horizontal position
A
  1. To increase the surface area of the liquid-air interface
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16
Q
  1. An open system in which the growth rate is maintained by the removal and addition of media at such a rate as to maintain a constant cell density is called
A
  1. Turbidostat
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17
Q
  1. Wash out in steady state fermentation occurs when
A
  1. Dilution rate is higher than the maximum specific growth rate
18
Q
  1. Fermenter should be filled with medium upto
19
Q
  1. Bacterial growth curve is obtained by plotting
A
  1. Log of number of cells versus time
20
Q
  1. When intracellular enzymes of whole cells are to be used in a bio-conversion process, it is often necessary to ______ the cells.
A
  1. Permeabilize
21
Q
  1. Heat transfer rates (per unit volume) will be lowest in
A
  1. Continuous packed bed reactor
22
Q
  1. The function(s) of the draft tube in an airlift bioreactor is
A
  1. To reduce bubble coalescence
23
Q
  1. The Monod Model relates
A
  1. Specific growth rate to the substrate availability
24
Q
  1. The height to diameter ratio (H/D) for the column fermenters is
25
Q
  1. The high oxygen transfer efficiencies of airlift bioreactors is due to
A
  1. The draft tube, which reduces bubble coalescence in the reactor
26
Q
  1. The maximum specific growth rate (μm) of an organism in batch culture is equal to slope of a plot of
A
  1. In [biomass] against time
27
Q
  1. The region of an airlift bioreactor in which the liquid travels in a downward direction is called the
28
Q
  1. Population doubling time, td can be expressed as
29
Q
  1. A higher Ks value of Monod’s equation means
A
  1. Lower affinities to substrate
30
Q
  1. The increased air flow rate in bubble column fermenter can cause excessive foaming and high retention of air bubbles in the column which
A
  1. Decreases the productivity of the fermenter
31
Q
  1. Which of the following is correct for the prediction of time requirement in a batch fermenter?
A
  1. Both (a) and (b)
32
Q
  1. In an airlift bioreactor, the spent gases released from the liquid are called
33
Q
  1. The contents in jet loop reactor are mixed through
A
  1. External pumping
34
Q
  1. The specific growth rate (μ) is defined as
A
  1. The rate of individual cells division or increase in their biomass
35
Q
  1. The number of baffles in a standard stirred tank bioreactor is
36
Q
  1. In the stationary phase
A
  1. Growth rate is equal to death rate
37
Q
  1. Which of the following is an example of a mathematical model?
A
  1. The Monod Model
38
Q
  1. Biomass concentrations during fermentation is
A
  1. All of the above
39
Q
  1. Chemostats work on the principle of
A
  1. Maintaining continuous flow of nutrients
40
Q
  1. During the exponential phase the maximum specific growth rate equals specific growth rate as
A
  1. Concentration of the growth limiting substrate is much greater than the Monod constant

D2 (22 decks)

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