Biochem - Biochem - Enzyme and Kinetics [2]

Question 1

1. The ratio of the amount of a protein present in a sample, which is used as a measure of purification, is known as

Answer 1

1. Specific activity

Question 2

2. If a reaction occurs in the absence of inhibitor with rate v0 and in the presence of inhibitor with rate vi, the degree of inhibition is defined as

Answer 2

2. (ν0−νi)/ν0(ν0​−νi​)/ν0​

Question 3

3. The rate equation in competitive inhibition based on Michaelis Menten equation is given by

Answer 3

3. rmax⁡S/(Kmt(1+I/Ki)+S))rmax​S/(Kmt​(1+I/Ki​)+S))

Question 4

4. Classical noncompetitive inhibition is obtained only under

Answer 4

4. Rapid equilibrium conditions

Question 5

5. In the steady state the material balance equation for any component of a system is

Answer 5

5. Rate of addition + rate of removal - rate of formation = 0

Question 6

6. For an enzyme that displays Michaelis-Menten kinetics, the reaction velocity (as a fraction of Vmax) observed at [S] = 2 KM will be

Answer 6

6. 0.66

Question 7

7. Predominantly uncompetitive inhibition may be called when

Answer 7

7. Competitive inhibition is smaller than uncompetitive inhibition

Question 8

8. An enzyme has a Km of 4.7 x 10-5M. If the Vmax of the preparation is 22m moles liter-1 min-1, what velocity would be observed in the presence of 2.0 x 10-4M substrate and 5.0 x 10-5M of a competitive inhibitor?

Answer 8

8. 13.54μ moles liter-1min-1

Question 9

9. The rate equation in non-competitive inhibition based on Michaelis Menten equation is given by

Answer 9

9. rmax⁡S/(Km+S)(1+I/Ki)rmax​S/(Km​+S)(1+I/Ki​)

Question 10

10. Which of the following statement(s) regarding enzymes, is/are false?

Answer 10

10. Enzymes provide activation energy for reactions

Question 11

11. The slope of Lineweaver Burk plot for Michaelis Menten equation is

Answer 11

11. KmVmax⁡Vmax​Km​​

Question 12

12. The initial velocity, V0, of an enzyme catalyzed reaction reaches Vmax as

Answer 12

12. [S]=10∗KM[S]=10∗KM​

Question 13

13. The usual method(s) to solve rate equation of simple enzyme kinetics is/are

Answer 13

13. All of these

Question 14

14. Michaelis Menten equation can also be written as

Answer 14

14. (-Cs)/r = (Cs/rmax)+(Km/rmax)

Question 15

15. Which of the following step is assumed to be the slowest step in the Michaelis Menten equation?

Answer 15

15. The product releasing step

Question 16

16. When substrate [S] = KM (Michaelis-Menten constant), the velocity of an enzyme catalyzed reaction is about

Answer 16

16. 0.5 * Vmax

Question 17

17. A classical noncompetitive inhibitor has

Answer 17

17. No effect on substrate binding

Question 18

18. The active site of an enzyme differs from an antibody-antigen binding site in that the enzyme active site

Answer 18

18. Catalyzes a chemical reaction

Question 19

19. Enzymes are basically

Answer 19

19. Proteins

Question 20

20. Which of the following refers to pseudo steady state?

Answer 20

20. d(CES)/dt = 0

Question 21

21. Most enzymes work by

Answer 21

21. Decreasing energy of activation

Question 22

22. Which category of enzymes belongs to class 5 in the international classification?

Answer 22

22. Isomerases

Question 23

23. Lock and key theory is based on the compatibility of

Answer 23

23. Enzyme and substrate

Question 24

24. When an enzyme is functioning at Vmax, the rate of the reaction is limited by

Answer 24

24. The rate at which the enzyme can convert substrate to product

Question 25

25. The equation for the rate of product formation for simple enzyme reaction is given by

Answer 25

25. rp=rmax Cv/(Km+Cs)rp​=rmax​Cv​/(Km​+Cs​)

Question 26

26. When [S] = 0.1 * KM, the velocity of an enzyme catalyzed reaction is about

Answer 26

26. 0.1 * Vmax