Biochem - Downstream Processing Flashcards
1
Q
- Concentration polarization can be reduced further by
A
- All of the above
2
Q
- A system which requires less solvent and produces a more concentrated extract phase, is desired with a
A
- Large distribution coefficients
3
Q
- The efficiency of cell disruption in a bead mill depends on the
A
- All of these
4
Q
- A common filter medium is the cloth filter generally made of
A
- All of these
5
Q
- An ion exchange resin is composed of
A
- All of these
6
Q
- Maintenance cost varies from
A
- 4-8% of the capital cost
7
Q
- The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between
A
- 15-30% by volume
8
Q
- Assuming the laminar flow across the filter, the rate of filtration (dVf/dt) can be expressed as a function of pressure drop Dp by the modified D’Arcy’s equation as
A
- (1/A)dVf/dt=Δpμ(L/K)dVf/dt(1/A)=μ(L/K)Δp
9
Q
- Filtration leaf test results indicate that the filtration rate of a protein product is 100 dry lbs/(ft22hr). What size production filter would be required to obtain 100 dry lbs of filter cake/hr?
A
- 1.54 ft2
10
Q
- Selectivity can be expressed as
A
- Ratio of the distribution coefficients in different solute
11
Q
- The disk centrifuge is the type of centrifuge used most often for bio separations due to its
A
- Continuous operation
12
Q
- The effectiveness of a solvent can be measured by the
A
- Both (a) and (b)
13
Q
- The stage wise operation of adsorption is called
A
- Conventional adsorption
14
Q
- Which of the following is not the physical method for the cells rupturing?
A
- Enzymatic digestion
15
Q
- Conventional adsorption is a
A
- Reversible process
16
Q
- If the solute passes through membrane freely, the the rejection coefficient (s) is
A
- 0
17
Q
- If the membrane rejects all solute, the rejection coefficient (s) is
A
- 1
18
Q
- In antibiotic manufacturing processes, the fermentation time ranges from
A
- 2-4 weeks
19
Q
- The conventional filtration involves the separation of large particles generally
A
- dp>5μm
20
Q
- Which of the operation does not come under upstream processing?
A
- Effluent treatment
21
Q
- For cost analysis in a fermenter system, the depreciation cost varies from
A
- 6-10% of the capital cost
22
Q
- Micro filtration refers to the separation of suspended material such as bacteria by using a membrane with spore sizes of
A
- 0.02 to 10μm
23
Q
- Start up expenses in a fermentation industry varies from
A
- 5-10% of the capital cost
24
Q
- The solvent flux across the membrane can be expressed as
A
- J = Lp(Δp - σΔπ)
25
Q
- Which of the following is considered as a pretreatment to the biological feeds?
A
- All of the above
26
Q
- For the extractions operation, the selectivity should be
A
- 1
27
Q
- The purity of a solute collected between two times t1 and t2 during chromatographic separation can be calculated as
A
- Amount of solute eluted / amount of impurity eluted
28
Q
- Chromatography is based on the
A
- Different rate of movement of the solute in the column
29
Q
- The correlation often employed to correlate adsorption data for protein is (where Ymax is the maximum amount of solute adsorbed per mass of adsorbent, X is the mass fraction of solute in the diluent phase in solute-free basis, KL is a constant and Y is the equilibrium value of the mass of solute adsorbed per mass of adsorbent)
A
- Y = YmaxX/(KL+X)
30
Q
- A typical tubular centrifuge has a bowl of 2 to 5 inch in diameter and 9 to 30 inch in height with a maximum rotating speed of
A
- 15,000 to 50,000 rpm
