Biochem Exam 3: Oxidative Phosphorylation

Question 1

How many membranes does the mitochondria have?

Answer 1

2
Inner
Outer

Question 2

How many compartments does a mitochondria have?

Answer 2

2
Intermembrane space
Matrix (site of TCA)

Question 3

How is mitochondria DNA inherited?

Answer 3

Maternally

Question 4

Where does oxidative phosphoralaytion happen?

Answer 4

Inner membrane of mitochondria

Question 5

Which membrane is permeable in the mitochondria?

Answer 5

Outer membrane

Question 6

What energy carrier molecules does the TCA generate

Answer 6

FADH2

NADH

Question 7

How many complexes does the ETC have?

Answer 7

4

Question 8

What three complexes pump protons?

Answer 8

I, III, IV

Question 9

What complex doen’t pump protons?

Answer 9

II

Question 10

How do protons return to the matrix after being pumped into the intermembrane space?

Answer 10

ATP synthase complex

Question 11

What are the three things oxPhos must accomplish to be successful?

Answer 11

1. Transfer electrons to oxygen
2. Establish a proton gradient
3. Synthesize ATP

Question 12

NADH Q Oxidoreductase is known as what complex?

Answer 12

Complex 1

Question 13

Q cytochrome C oxidoreductase is known as what complex?

Answer 13

Complex III

Question 14

Cytochrome c oxidase is known as what complex?

Answer 14

Complex IV

Question 15

Succinate Q reductase is what complex?

Answer 15

II

Question 16

T/F

Succinate Q reductase does not pump protons

Answer 16

True

Question 17

Coenzyme Q is hydrophobic and transfers electrons from what complexes?

Answer 17

I & II

Question 18

What is the most common co enzyme Q aka ubiquinone?

Answer 18

CoQ 10

Question 19

Cytochrome C shuttle electrons from __________to _____________

Answer 19

complex III to IV

Question 20

What catalyzes the reduction of O2?

Answer 20

Cytochrome C

Question 21

Electrons flow ___________ in the ETC

Answer 21

Exergonic

Complex I = neg, complex IV = pos

Question 22

Does FADH2 leave complex II?

Answer 22

No

Question 23

T/F

Electrons transferred to FeS and then to Q to form QH2 in complex II

Answer 23

True

Question 24

How many protons are pumpsed to the cytosol from complex III?

Answer 24

2 protons

Question 25

How many electrons are funneled to oxygen in complex IV?

Answer 25

4

Question 26

ROS stands for ___________

Answer 26

Reactive oxygen species

Question 27

Reactive oxygen species are ______________

Answer 27

Partial reduced oxygen

Question 28

R.O.S. Include:

Answer 28

Superoxide ion
Peroxide ion
Hydroxyl radical

Question 29

What antioxidant can take superoxide ion to hydrogen peroxide

Answer 29

Superoxide dismutase

Question 30

What antioxidant can take peroxide and create water?

Answer 30

Catalase

Question 31

List 5 antioxidants

Answer 31

1. Superoxide dismutase (SOD)
2. Catalase
3. Glutathione peroxidase
4. Vit E
5. Vit C

Question 32

How does superoxide dismutase using CU/ZN is found where?

Answer 32

Cytosolic

Question 33

Superoxide dismutase using Mn/Zn is found where?

Answer 33

Mitochondrial