Biochem- Biotechnology

Question 1

which protein quantification method separates proteins based on electrophoretic ability?

Answer 1

SDS page

Question 2

which protein quantification method separates proteins based on affinity for stationary or mobile phase?

Answer 2

chromatography

Question 3

affinity vs column chromatography?

Answer 3

affinity - uses a bound receptor ligand an an eluent with free ligand or receptor for protein of interest
column- uses beads of a polar compound (stationary phase) with a nonpolar solvent ( mobile phase)

Question 4

which protein quantification method separates proteins based on overall charge?

Answer 4

Ion exchange

Question 5

which protein quantification method separates proteins based on size? Order of elution?

Answer 5

SEC- Size Exclusion , uses porous beads, large molecules elute first, since they don’t get trapped in the porous beads