Biochem 82

Question 1

Southern blotting uses what to identify the target gene?

UW: 2034

Answer 1

radiolabeled DNA probe

Question 2

Which blotting technique is useful to understand gene expression?

Answer 2

Northern Blot

Question 3

Western blot is a confirmatory test for what?

Answer 3

HIV after positive ELISA

Question 4

What is southwestern blotting?

Answer 4

used to identify DNA binding proteins (e.g. transcription factors) using labeled oligonucleotide probes

Question 5

What are some examples of transcription factors that can be identified by southwestern blotting?

Answer 5

c-Jun and c-Fos

Question 6

What is the use of microarrays?

Answer 6

Used to profile gene expression levels of thousands of genes simultaneously

Question 7

Microarrays can detect what?

Answer 7

SNPs and CNVs

Question 8

What is the purpose of an ELISA test?

Answer 8

used to identify the presence of a specific antigen (direct) or a specific antibody (indirect) in pt blood sample

Question 9

How does one know that an ELISA test is positive?

Answer 9

Test solution will have an intense color