Biochem 66

Question 1

Histones are rich in which amino acids?

Answer 1

• Arginine

- Lysine

Question 2

DNA and histone synthesis occur during which phase?

Answer 2

S phase of mitosis

Question 3

Which is the only histone located outside the nucleosome core?

Answer 3

H1

Question 4

Describe heterochromatin

Answer 4

Condensed, transcriptionally inactive, sterically inaccessible, and heavily methylated.

Question 5

Describe euchromatin

Answer 5

Less condesed, transcriptionally active, sterically accessible. Histones acetylated.

Question 6

DNA methylation where represses transcription?

Answer 6

CpG isalnds.

Question 7

What process relaxes DNA coiling, allowing for transcription?

Answer 7

Histone Acetylation

Question 8

Which nucleotides on the template strand are methylated in DNA replication (2)

Answer 8

Cytosine

Adenine

Question 9

Which nucelotide has a methyl group?

Answer 9

Thymine

Question 10

Which process makes cytosine into uracil?

Answer 10

deamination

Question 11

How does one increase the melting temperature of DNA?

Answer 11

Increase C-G content

Question 12

Which amino acids are necessary for purine synthesis? (3)

Answer 12

• Glycine
• Aspartate
• Glutamine

Question 13

Nucleotides are linked by what bond?

Answer 13

3-5 phosphodiester bond