Biochem 74

Question 1

Abnormal splicing variants are implicated in oncogenesis and many genetics disorders such as what?

Answer 1

B-thalassemia

Question 2

tRNA is made of how many nucleotides?

Answer 2

75-90

Question 3

What is the sequence of the amino acid acceptor site on the 3’ end of the tRNA?

Answer 3

CCA

Can Carry Amino acids

Question 4

What is contained on the T arm of the tRNA?

Answer 4

Thymine, Pseudouridine, Cytosine

tRNA is only species with thymidine. U.W.

Question 5

What is the function of the T arm of tRNA?

Answer 5

tRNA ribosome binding

Question 6

The D arm of tRNA contains what? Which is necessary for what?

Answer 6

• Dihydrouracil

- tRNA recognition by the correct aminoacyl-tRNA synthetase

Question 7

Which enzyme charges tRNA?

Answer 7

Aminoacyl-tRNA synthetase (uses ATP)

Question 8

The amino acid tRNA has energy for the formation of what bond?

Answer 8

Peptide bond

Question 9

The anticodon is read in what direction?

Answer 9

3’ - 5’

So, AUG codon would be UAC, but written CAU.