Biochem 78 Flashcards
Describe the composition of microtubules
polymerized heterodimers composed of alpha and beta-tubulin.
Each dimer of a microtubule is bound by what?
2 GTP molecules
Microtubules are incorporated into what? (3) Involved in? (2)
1) Flagella
2) Cilia
3) Mitotic spindles
involved in:
1) axonal trafficking
2) Centrioles
Microtubules are a component of what in neurons?
Slow axonal transport
Which proteins are involved in axon transport along microtubules in neurons?
Dynein (retrograde + to -)
Kinesis (anterograde - to +)
Which drugs act on microtubules? (5)
1) Mebendazole (anti-helminthic)
2) Griseofulvin (anti-fungal)
3) Cochicine (anti-gout)
4) Vincristine / Vinblastine (anti-cancer)
5) Paclitaxel (anti-cancer)
In regards to cilia structure, how are the microtubules arranged?
9 + 2
What is Axonemal dyenin?
ATPase that links peripheral 9 doublets and causes bending of cilium by differential sliding of doublets.
Kartagener syndrome is also known as what?
Primary ciliary dyskinesia
Kartagener’s syndrome is due to what?
dyenin arm defect
How does Kartagener’s syndrome cause female infertility?
dysfunctional fallopian tube cilia -> increased risk of ectopic pregnancy
What signs can be seen with Kartagener’s syndrome?
1) Bronchiectasis
2) Recurrent sinusitis
3) Situs inversus (e.g. dextrocardia on CXR)
Actins are?
Long, structural polymers
Myosin’s are?
Dimeric ATP driven proteins that move along motor chains
List the various types of intermediate filaments: (6)
1) Vimentin
2) Desmin
3) Cytokeratin
4) Lamins
5) Glial Fibrially Acid Protein (GFAP)
6) Neurofilaments
