Biochem 3

Question 1

Creating antibodies

Answer 1

-use analog of TS

Question 2

Catalyst vs deltaG

Answer 2

a catalyst allows a high Ea rxn to occur but it should still have a negative deltaG/deltaH to allow the rxn to occur

spontanoeus = neg deltaG = negdeltaH and pos deltaS = exothermic

Question 3

Decrease expression by methylation on

Answer 3

promoters, enhancers, CpG islands

CpG is IN the promoter

enhancer helps RNA Poly bind at the promoter

Question 4

uncouplers/protonophores

Answer 4

-let H+ leak through = decrease efficiency = SPEED metabolism

Question 5

Ligase

Answer 5

• synthesis b/w 2 large molecules

- need ATP

Question 6

amylose degradation

Answer 6

• b-amylase cleaves at non-reducing end to make maltose; it only digests amylose but not amylopectin bc it can’t deal w the branching
• a-amylase cleaves randomly = can cleave amylose or amylopectin

Question 7

DNA stability

Answer 7

-stacking van der Waals interactions b/w adjacent bases on each of the antiparallel strands

AVTVA
TVAVT

antiparallal strands, the V are the van der waals

Question 8

Stacking

Answer 8

van der waals

Question 9

Gq

Answer 9

IP3

Question 10

Gs / Gi

Answer 10

cAMP

Question 11

Hypothyroidism

Answer 11

= less lipid metabolic products
= decreased amount of Acetyl CoA, malonyl CoA, and glycerol
= increased cholesterol b/c you can’t break it down